Development of electrospun nanofibers with suitable properties to promote wound healing is an advantage in developing non-invasive skin treatments. We showed the potential application of Polyvinyl acetate (PVA) and Polyvinylpyrrolidone (PVP) combined with Helichrysum italicum oil (HO) in wound healing. During this process, Tight junctions (TJs) play a crucial role in maintaining skin integrity. TJs are intercellular junctions composed of a variety of transmembrane proteins, including Occludin (OCLN), observed also in migrating epithelial cells. Changes in OCLN expression affect epidermal permeability, indicating an active role in the healing process. Within this context, we studied the OCLN expression during healing after scratch assay on Keratinocytes (HaCaT), by a confocal microscopic analysis. In addition, we evaluated the effect of treatment after scratch on cell elasticity by Atomic Force Microscopy (AFM) analysis. All results show a positive trend in cell proliferation and viability on HaCaT treated with functionalized nanofibers. These results were confirmed by the expression of genes involved in the early stages of the regenerative process. Understanding the cell mechanisms involved in skin changes during repair process would allow future application of nanomaterials combined with HO in vivo.

• Klíčová slova
• Bioactive molecules, Cellular mechanism, Keratinocytes, Nanofibers, Regenerative medicine, Tissue regeneration, Wound healing,
• MeSH
• buněčné linie MeSH
• hojení ran účinky léků   MeSH
• keratinocyty * účinky léků   metabolismus   cytologie   MeSH
• kůže účinky léků   metabolismus   MeSH
• lidé MeSH
• mikroskopie atomárních sil MeSH
• nanovlákna * chemie   MeSH
• okludin metabolismus   genetika   MeSH
• oleje rostlin farmakologie   chemie   MeSH
• polyvinylalkohol chemie   MeSH
• povidon * chemie   farmakologie   MeSH
• proliferace buněk * účinky léků   MeSH
• pružnost účinky léků   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• okludin MeSH
• oleje rostlin MeSH
• polyvinylalkohol MeSH
• povidon * MeSH

Neural injuries in cerebral malaria patients are a significant cause of morbidity and mortality. Nevertheless, a comprehensive research approach to study this issue is lacking, so herein we propose an in vitro system to study human cerebral malaria using cellular approaches. Our first goal was to establish a cellular system to identify the molecular alterations in human brain vasculature cells that resemble the blood-brain barrier (BBB) in cerebral malaria (CM). Through transcriptomic analysis, we characterized specific gene expression profiles in human brain microvascular endothelial cells (HBMEC) activated by the Plasmodium falciparum parasites. We also suggest potential new genes related to parasitic activation. Then, we studied its impact at brain level after Plasmodium falciparum endothelial activation to gain a deeper understanding of the physiological mechanisms underlying CM. For that, the impact of HBMEC-P. falciparum-activated secretomes was evaluated in human brain organoids. Our results support the reliability of in vitro cellular models developed to mimic CM in several aspects. These systems can be of extreme importance to investigate the factors (parasitological and host) influencing CM, contributing to a molecular understanding of pathogenesis, brain injury, and dysfunction.

• Klíčová slova
• HBMEC activation, brain organoids, cerebral malaria, human iPSCs, secretome, transcriptome,
• MeSH
• endoteliální buňky metabolismus   MeSH
• lidé MeSH
• mozek patologie   MeSH
• mozková malárie * metabolismus   parazitologie   patologie   MeSH
• organoidy metabolismus   MeSH
• Plasmodium falciparum MeSH
• reprodukovatelnost výsledků MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH