The aim of this study was to evaluate the bioactive substances in 19 berry cultivars of edible honeysuckle (Lonicera edulis). A statistical evaluation was used to determine the relationship between the content of selected bioactive substances and individual cultivars. Regarding mineral elements, the content of sodium was measured using potentiometry and spectrophotometry. The content of selected polyphenolic compounds with high antioxidant activity was determined by a HPLC-UV/ED method. The total amount of polyphenols was determined by the Folin-Ciocalteu method. The antioxidant activity was determined using five methods (DPPH, FRAP, ABTS, FR and DMPD) that differ in their principles. The content of 13 amino acids was determined by ion-exchange chromatography. The experimental results obtained for the different cultivars were evaluated and compared by statistical and bioinformatic methods. A unique feature of this study lies in the exhaustive analysis of the chosen parameters (amino acids, mineral elements, polyphenolic compounds and antioxidant activity) during one growing season.

• MeSH
• aminokyseliny analýza   MeSH
• antioxidancia chemie   farmakologie   MeSH
• chromatografie iontoměničová MeSH
• genotyp MeSH
• Lonicera chemie   genetika   MeSH
• minerály analýza   MeSH
• ovoce chemie   MeSH
• polyfenoly analýza   chemie   MeSH
• shluková analýza MeSH
• vysokoúčinná kapalinová chromatografie metody   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• aminokyseliny MeSH
• antioxidancia MeSH
• minerály MeSH
• polyfenoly MeSH

Dry matter, organic acids, ascorbic acid, minerals (nitrogen, phosphorus, potassium, calcium, magnesium, sodium) and polyphenolic profile of a number of non-traditional fruit species and their genotypes, namely blue honeysuckle (Lonicera spp.), Saskatoon berry (Amelanchier alnifolia), black mulberry (Morus nigra), Tomentosa cherry (Prunus tomentosa Thunb.) and jostaberry (Ribes nigrum x Grossularia uva-crispa) were investigated. The results showed that Lonicera genotypes displayed high levels of ascorbic acid and they were rich in minerals, with the cultivar ‘Amfora’ achieving the leading position in nitrogen, phosphorus and potassium content among all lesser known fruit species. Amelanchier cultivars represented a valuable source of ascorbic acid and calcium, ‘Tišňovský’ and ‘Smoky’ together with Morus nigra ‘Jugoslavska’ accumulated the highest level of examined polyphenolic compounds. Regular consumption of studied less common fruit species can bring health benefits so they can represent a high potential value for fruit growers and in addition they can be utilised as functional foods.

• MeSH
• genotyp MeSH
• kyseliny karboxylové analýza   izolace a purifikace   MeSH
• kyseliny nekarboxylové analýza   izolace a purifikace   MeSH
• Lonicera chemie   MeSH
• minerály analýza   izolace a purifikace   MeSH
• Morus chemie   MeSH
• nutriční hodnota * MeSH
• ovoce chemie   MeSH
• polyfenoly analýza   izolace a purifikace   MeSH
• Ribes chemie   MeSH
• Rosaceae chemie   MeSH
• rostlinné extrakty analýza   izolace a purifikace   MeSH
• slivoň chemie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Geografické názvy
• Česká republika MeSH
• Názvy látek
• kyseliny karboxylové MeSH
• kyseliny nekarboxylové MeSH
• minerály MeSH
• polyfenoly MeSH
• rostlinné extrakty MeSH

Experimental studies have shown that phenolic compounds have antiproliferative and tumour arresting effects. The aim of this original study was to investigate the content of phenolic compounds (PhC) in flowers of Allium schoenoprasum (chive), Tragopogon pratensis (meadow salsify) and Rumex acetosa (common sorrel) and their effect on proliferation of HaCaT cells. Antiproliferative effects were evaluated in vitro using the following concentrations of phenolic compounds in cultivation medium: 100, 75, 50 and 25 µg/mL. Phenolic composition was also determined by HPLC. The results indicate that even low concentrations of these flowers' phenolic compounds inhibited cell proliferation significantly and the possible use of the studied herb's flowers as sources of active phenolic compounds for human nutrition.

• MeSH
• antitumorózní látky chemie   farmakologie   MeSH
• fenoly chemie   farmakologie   MeSH
• květy chemie   MeSH
• léky antitumorózní - screeningové testy MeSH
• lidé MeSH
• nádorové buněčné linie účinky léků   MeSH
• pažitka anatomie a histologie   chemie   MeSH
• rostlinné extrakty chemie   farmakologie   MeSH
• Rumex anatomie a histologie   chemie   MeSH
• Tragopogon anatomie a histologie   chemie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antitumorózní látky MeSH
• fenoly MeSH
• rostlinné extrakty MeSH

Functional foods are of interest because of their significant effects on human health, which can be connected with the presence of some biologically important compounds. In this study, we carried out complex analysis of 239 apricot cultivars (Prunus armeniaca L.) cultivated in Lednice (climatic area T4), South Moravia, Czech Republic. Almost all previously published studies have focused only on analysis of certain parameters. However, we focused on detection both primary and secondary metabolites in a selection of apricot cultivars with respect to their biological activity. The contents of thirteen biogenic alpha-L-amino acids (arginine, asparagine, isoleucine, lysine, serine, threonine, valine, leucine, phenylalanine, tryptophan, tyrosine, proline and alanine) were determined using ion exchange chromatography with UV-Vis spectrometry detection. Profile of polyphenols, measured as content of ten polyphenols with significant antioxidant properties (gallic acid, procatechinic acid, p-aminobenzoic acid, chlorogenic acid, caffeic acid, vanillin, p-coumaric acid, rutin, ferrulic acid and quercetrin), was determined by high performance liquid chromatography with spectrometric/electrochemical detection. Moreover, content of total phenolics was determined spectrophotometrically using the Folin-Ciocalteu method. Antioxidant activity was determined using five independent spectrophotometric methods: DPPH assay, DMPD method, ABTS method, FRAP and Free Radicals methods. Considering the complexity of the obtained data, they were processed and correlated using bioinformatics techniques (cluster analysis, principal component analysis). The studied apricot cultivars were clustered according to their common biochemical properties, which has not been done before. The observed similarities and differences were discussed.

• MeSH
• algoritmy MeSH
• aminokyseliny chemie   MeSH
• analýza hlavních komponent * MeSH
• antioxidancia chemie   MeSH
• benzothiazoly chemie   MeSH
• bifenylové sloučeniny chemie   MeSH
• genový pool MeSH
• kyseliny sulfonové chemie   MeSH
• ovoce chemie   MeSH
• pikráty chemie   MeSH
• polyfenoly chemie   MeSH
• rostlinné extrakty chemie   MeSH
• shluková analýza MeSH
• slivoň chemie   MeSH
• volné radikály chemie   MeSH
• výpočetní biologie MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• 1,1-diphenyl-2-picrylhydrazyl MeSH Prohlížeč
• 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid MeSH Prohlížeč
• aminokyseliny MeSH
• antioxidancia MeSH
• benzothiazoly MeSH
• bifenylové sloučeniny MeSH
• kyseliny sulfonové MeSH
• pikráty MeSH
• polyfenoly MeSH
• rostlinné extrakty MeSH
• volné radikály MeSH