Molecular cloning, nucleotide sequencing, and characterization of the flaA gene from additional isolates of urease-positive thermophilic Campylobacter (UPTC) were performed. These isolates were obtained from the natural environment in Northern Ireland (n = 9 from mussels) and in England (n = 1 from sea water). All isolates carried the shorter flaA gene, [open reading frames (ORFs), 1,461 to 1,503 base pairs], without any internal termination codons, and did not carry any flaA pseudogenes. The UPTC isolates were well discriminated by the neighbor joining (NJ) phylogenetic tree constructed based on the putative flaA genes ORFs nucleotide sequence information. In addition, the NJ tree constructed based on the flaA-short variable region sequence information discriminated the Campylobacter lari isolates with a similar degree of discrimination power.

• MeSH
• Campylobacter lari classification   genetics   isolation & purification   metabolism   MeSH
• Campylobacter classification   genetics   isolation & purification   metabolism   MeSH
• Flagellin chemistry   genetics   metabolism   MeSH
• Phylogeny MeSH
• Cloning, Molecular MeSH
• Bivalvia microbiology   MeSH
• Molecular Sequence Data MeSH
• Seawater microbiology   MeSH
• Open Reading Frames MeSH
• Polymerase Chain Reaction MeSH
• Recombinant Proteins chemistry   genetics   metabolism   MeSH
• Amino Acid Sequence MeSH
• Sequence Analysis, DNA methods   MeSH
• Sequence Alignment MeSH
• Urease metabolism   MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Geographicals
• England MeSH
• Northern Ireland MeSH
• Names of Substances
• flaA protein, bacteria MeSH Browser
• Flagellin MeSH
• Recombinant Proteins MeSH
• Urease MeSH