Using supramolecular self-assembled nanocomposite materials made from protein and polysaccharide components is becoming more popular because of their unique properties, such as biodegradability, hierarchical structures, and tunable multifunctionality. However, the fabrication of these materials in a reproducible way remains a challenge. This study presents a new evaporation-induced self-assembly method producing layered hydrogel membranes (LHMs) using tropocollagen grafted by partially deacetylated chitin nanocrystals (CO-g-ChNCs). ChNCs help stabilize tropocollagen's helical conformation and fibrillar structure by forming a hierarchical microstructure through chemical and physical interactions. The LHMs show improved mechanical properties, cytocompatibility, and the ability to control drug release using octenidine dihydrochloride (OCT) as a drug model. Because of the high synergetic performance between CO and ChNCs, the modulus, strength, and toughness increased significantly compared to native CO. The biocompatibility of LHM was tested using the normal human dermal fibroblast (NHDF) and the human osteosarcoma cell line (Saos-2). Cytocompatibility and cell adhesion improved with the introduction of ChNCs. The extracted ChNCs are used as a reinforcing nanofiller to enhance the performance properties of tropocollagen hydrogel membranes and provide new insights into the design of novel LHMs that could be used for various medical applications, such as control of drug release in the skin and bone tissue regeneration.

• MeSH
• biokompatibilní materiály * chemie   MeSH
• chitin * chemie   MeSH
• fibroblasty MeSH
• hydrogely * chemie   MeSH
• iminy farmakokinetika   MeSH
• konformace proteinů, alfa-helix MeSH
• léky s prodlouženým účinkem * chemie   MeSH
• lidé MeSH
• mechanické jevy MeSH
• membrány chemie   MeSH
• nádorové buněčné linie MeSH
• nanočástice chemie   MeSH
• nanokompozity * chemie   MeSH
• pyridiny farmakokinetika   MeSH
• tropokolagen * chemie   MeSH
• uvolňování léčiv MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• biokompatibilní materiály * MeSH
• chitin * MeSH
• hydrogely * MeSH
• iminy MeSH
• léky s prodlouženým účinkem * MeSH
• octenidine MeSH Prohlížeč
• pyridiny MeSH
• tropokolagen * MeSH

We report the preparation, characterisation and in vitro tests of hyaluronan fibres containing up to 50 w/w% of temozolomide for local glyoblastoma treatment. These fibres form a hydrogel upon contact with cerebrospinal fluid on the treatment spot.

• Publikační typ
• časopisecké články MeSH

The marine-derived hyaluronic acid and other natural biopolymers offer exciting possibilities in the field of biomaterials, providing sustainable and biocompatible alternatives to synthetic materials. Their unique properties and abundance in marine sources make them valuable resources for various biomedical and industrial applications. Due to high biocompatible features and participation in biological processes related to tissue healing, hyaluronic acid has become widely used in tissue engineering applications, especially in the wound healing process. The present review enlightens marine hyaluronan biomaterial providing its sources, extraction process, structures, chemical modifications, biological properties, and biocidal applications, especially for wound healing/dressing purposes. Meanwhile, we point out the future development of wound healing/dressing based on hyaluronan and its composites and potential challenges.

• Klíčová slova
• characterization, composites, extraction process, hyaluronan, marine sources, wound healing applications,
• MeSH
• biokompatibilní materiály farmakologie   MeSH
• hojení ran MeSH
• kyselina hyaluronová * farmakologie   MeSH
• obvazy * MeSH
• tkáňové inženýrství MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• biokompatibilní materiály MeSH
• kyselina hyaluronová * MeSH

Wounds are structural and functional disruptions of skin that occur because of trauma, surgery, acute illness, or chronic disease conditions. Chronic wounds are caused by a breakdown in the finely coordinated cascade of events that occurs during healing. Wound healing is a long process that split into at least three continuous and overlapping processes: an inflammatory response, a proliferative phase, and finally the tissue remodeling. Therefore, these processes are extensively studied to develop novel therapeutics in order to achieve maximum recovery with minimum scarring. Several growth hormones and cytokines secreted at the site of lesions tightly regulates the healing processes. The traditional approach for wound management has been represented by topical treatments. Metal nanoparticles (e.g., silver, gold and zinc) are increasingly being employed in dermatology due to their favorable effects on healing, as well as in treating and preventing secondary bacterial infections. In the current review, a brief introduction on traditional would healing approach is provided, followed by focus on the potential of wound dressing therapeutic techniques functionalized with Ag-NPs.

• Klíčová slova
• dermatology, inflammatory response, silver nanoparticles, wound, wound healing,
• MeSH
• antibakteriální látky terapeutické užití   MeSH
• hojení ran MeSH
• kovové nanočástice * chemie   terapeutické užití   MeSH
• obvazy MeSH
• stříbro * chemie   terapeutické užití   MeSH
• zlato chemie   MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• antibakteriální látky MeSH
• stříbro * MeSH
• zlato MeSH