Additives in vaping products, such as flavors, preservatives, or thickening agents, are commonly used to enhance user experience. Among these, Vitamin E acetate (VEA) was initially thought to be harmless but has been implicated as the primary cause of e-cigarette or vaping product use-associated lung injury, a serious lung disease. In our study, VEA serves as a proxy for other e-cigarette additives. To explore its harmful effects, we developed an exposure system to subject a pulmonary surfactant (PSurf) model to VEA-rich vapor. Through detailed analysis and atomic-level simulations, we found that VEA tends to cluster into aggregates on the PSurf surface, inducing deformations and weakening its essential elastic properties, critical for respiratory cycle function. Apart from VEA, our experiments also indicate that propylene glycol and vegetable glycerin, widely used in e-liquid mixtures, or their thermal decomposition products, alter surfactant properties. This research provides molecular-level insights into the detrimental impacts of vaping product additives on lung health.

• Klíčová slova
• EVALI, Lung surfactant, Molecular dynamics simulation, Pulmonary surfactant, Vaping-associated pulmonary injury,
• MeSH
• acetáty analýza   chemie   MeSH
• biologické modely MeSH
• lidé MeSH
• plicní surfaktanty * chemie   MeSH
• propylenglykol chemie   MeSH
• systémy dodávající nikotin elektronicky * MeSH
• vaping * škodlivé účinky   MeSH
• vitamin E * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• acetáty MeSH
• plicní surfaktanty * MeSH
• propylenglykol MeSH
• vitamin E * MeSH

SCOPE: The aim of this work is to identify which proapoptotic pathway is induced in human colon cancer cell lines, in contact with proanthocyanidins extracted from various berries. METHODS AND RESULTS: Proanthocyanidins (Pcys) extracted from 11 berry species are monitored for proapoptotic activities on two related human colon cancer cell lines: SW480-TRAIL-sensitive and SW620-TRAIL-resistant. Apoptosis induction is monitored by cell surface phosphatidylserine (PS) detection. Lowbush blueberry extract triggers the strongest activity. When tested on the human monocytic cell line THP-1, blueberry Pcys are less effective for PS externalisation and DNA fragmentation is absent, highlighting a specificity of apoptosis induction in gut cells. In Pcys-treated gut cell lines, caspase 8 (apoptosis extrinsic pathway) but not caspase 9 (apoptosis intrinsic pathway) is activated after 3 hours through P38 phosphorylation (90 min), emphasizing the potency of lowbush blueberry Pcys to eradicate gut TRAIL-resistant cancer cells. CONCLUSION: We highlight here that berries Pcys, especially lowbush blueberry Pcys, are of putative interest for nutritional chemoprevention of colorectal cancer in view of their apoptosis induction in a human colorectal cancer cell lines.

• MeSH
• antigeny CD95 metabolismus   MeSH
• apoptóza účinky léků   MeSH
• brusnice s jedlými plody chemie   metabolismus   MeSH
• buněčné linie MeSH
• chemorezistence MeSH
• DNA metabolismus   MeSH
• fosfatidylseriny metabolismus   MeSH
• fosforylace účinky léků   MeSH
• fragmentace DNA účinky léků   MeSH
• kaspasa 3 metabolismus   MeSH
• kaspasa 8 metabolismus   MeSH
• kaspasa 9 metabolismus   MeSH
• lidé MeSH
• mitogenem aktivované proteinkinasy p38 metabolismus   MeSH
• nádory tračníku metabolismus   patologie   MeSH
• ovoce chemie   metabolismus   MeSH
• proantokyanidiny chemie   izolace a purifikace   toxicita   MeSH
• protein TRAIL toxicita   MeSH
• rostlinné extrakty chemie   MeSH
• TRAIL receptory metabolismus   MeSH
• Vaccinium vitis-idaea chemie   metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny CD95 MeSH
• DNA MeSH
• FAS protein, human MeSH Prohlížeč
• fosfatidylseriny MeSH
• kaspasa 3 MeSH
• kaspasa 8 MeSH
• kaspasa 9 MeSH
• mitogenem aktivované proteinkinasy p38 MeSH
• proantokyanidiny MeSH
• protein TRAIL MeSH
• rostlinné extrakty MeSH
• TRAIL receptory MeSH