A new β-galactosidase-producing bacterium KGP, isolated from the Bay of Bengal, was identified as Paracoccus marcusii through morphology, biochemistry and 16S rRNA sequencing. This study is the first report on the production of β-galactosidase from P. marcusii. The medium components for the high yield of β-galactosidase were optimised using response surface methodology (RSM). A set of 17 experiments consisting of three independent variables, viz. yeast extract, galactose and MgSO4, was employed. A second-order polynomial equation was used for the analysis of the response, and the optimum β-galactosidase yield was achieved using 12.5 g/L yeast extract, 12.5 g/L galactose and 12.5 mmol/L MgSO4. The predicted quadratic model was inferred to be significant from the F-value, P value and the lack of fit value. Optimisation of the media components resulted in a ninefold increase (560 Miller units) in β-galactosidase production. Furthermore, the hydrolysis and transgalactosylation efficiency of the crude β-galactosidase was assessed and the results showed that the lactose was successfully hydrolysed and transgalactosylated at an optimum temperature of 40 °C and 50 °C, respectively. Considering the overall yield and productivity, P. marcusii can be considered a candidate for the industrial production of β-galactosidase. This study provides an essential basis for the future production and use of the alkali-tolerant β-galactosidase from P. marcusii KGP.

• MeSH
• beta-galaktosidasa metabolismus   MeSH
• bioreaktory MeSH
• fylogeneze MeSH
• galaktosa metabolismus   MeSH
• hydrolýza MeSH
• koncentrace vodíkových iontů MeSH
• laktosa metabolismus   MeSH
• oligosacharidy biosyntéza   MeSH
• Paracoccus klasifikace   genetika   izolace a purifikace   metabolismus   MeSH
• RNA ribozomální 16S genetika   MeSH
• teplota MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• beta-galaktosidasa MeSH
• galaktosa MeSH
• laktosa MeSH
• oligosacharidy MeSH
• RNA ribozomální 16S MeSH

Chemical analyses of Fusarium avenaceum grown on banana medium resulted in eight novel spiroleptosphols, T1, T2 and U-Z (1-8). The structures were elucidated by a combination of high-resolution mass spectrometric data and 1- and 2-D NMR experiments. The relative stereochemistry was assigned by 1H coupling and NOESY/ROESY experiments. Absolute stereochemistry established for 7 by vibrational circular dichroism was found analogous to that of the putative polyketide spiroleptosphol from Leptosphaeria doliolum.

• Klíčová slova
• Fusarium avenaceum, PKS, polyketide synthases, polyketides, secondary metabolites, spiroleptosphol,
• MeSH
• chemické jevy MeSH
• Fusarium chemie   metabolismus   MeSH
• magnetická rezonanční spektroskopie MeSH
• metabolické sítě a dráhy MeSH
• molekulární struktura MeSH
• spirosloučeniny chemie   metabolismus   MeSH
• vysokoúčinná kapalinová chromatografie MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• spirolephtoshol MeSH Prohlížeč
• spirosloučeniny MeSH