This study aimed to assess the toxicity of praziquantel (anthelmintic drug) in different developmental stages of common carp (Cyprinus carpio) based on mortality, early ontogeny, growth, oxidative stress, antioxidant enzymes, histology and behaviour. Praziquantel at all tested concentrations ranging from 1 to 4 mg/L showed no significant adverse effects on mortality, the early ontogeny and behaviour locomotory (activity, moved distance and velocity) of carp after 35-day exposure. Concentrations of 3 and 4 mg/L caused significantly (P < 0.01) lower growth, total superoxide dismutase and catalase activities compared with controls. Praziquantel is safe for the early life of carp in concentrations ≤ 2 mg/L.

• MeSH
• antioxidancia farmakologie   MeSH
• chemické látky znečišťující vodu * toxicita   MeSH
• embryo nesavčí MeSH
• kapři * MeSH
• katalasa farmakologie   MeSH
• larva MeSH
• praziquantel farmakologie   MeSH
• superoxiddismutasa farmakologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antioxidancia MeSH
• chemické látky znečišťující vodu * MeSH
• katalasa MeSH
• praziquantel MeSH
• superoxiddismutasa MeSH

Degradation products of herbicides, alone and in combination, may affect non-target aquatic organisms via leaching or runoff from the soil. The effects of 50-day exposure of primary metabolites of chloroacetamide herbicide, acetochlor ESA (AE; 4 µg/L), and glyphosate, aminomethylphosphonic acid (AMPA; 4 µg/L), and their combination (AMPA + AE; 4 + 4 µg/L) on mortality, growth, oxidative stress, antioxidant response, behaviour, and gill histology of early life stages of marbled crayfish (Procambarus virginalis) were investigated. While no treatment effects were observed on cumulative mortality or early ontogeny, growth was significantly lower in all exposed groups compared with the control group. Significant superoxide dismutase activity was observed in exposure groups, and significantly higher glutathione S-transferase activity only in the AMPA + AE group. The gill epithelium in AMPA + AE-exposed crayfish showed swelling as well as numerous unidentified fragments in interlamellar space. Velocity and distance moved in crayfish exposed to metabolites did not differ from controls, but increased activity was observed in the AMPA and AE groups. The study reveals the potential risks of glyphosate and acetochlor herbicide usage through their primary metabolites in the early life stages of marbled crayfish.

• Klíčová slova
• behaviour, crayfish, herbicide, metabolite, ontogeny, toxicity,
• Publikační typ
• časopisecké články MeSH

The sensitivity of early life stages of common carp (Cyprinus carpio L.) to chronic exposure to single and combined environmental concentrations of the triazine metabolites terbuthylazine 2-hydroxy, terbuthylazine-desethyl and atrazine 2-hydroxy was evaluated under laboratory conditions. Their effects were assessed on lipid peroxidation, antioxidant enzymes (total superoxide dismutase, glutathione reductase, catalase, glutathione S-transferase, reduced glutathione), mortality, growth, development and histology. Single metabolites (terbuthylazine 2-hydroxy-0.73 μg/L; terbuthylazine-desethyl-1.80 μg/L; atrazine 2-hydroxy-0.66 μg/L) and combinations were not associated with negative effects on hatching, behaviour, embryo viability, growth or early ontogeny. Carp exposed to terbuthylazine-desethyl at 1.80 μg/L showed significantly lower total superoxide dismutase and glutathione reductase activity compared with the control group. Liver histology revealed diffused steatosis associated with the presence of lipid inclusions in hepatic cells in groups exposed to terbuthylazine-desethyl, atrazine 2-hydroxy and the tested combination of metabolites.

• Klíčová slova
• Antioxidant enzymes, Atrazine 2-hydroxy, Fish, Terbuthylazine 2-hydroxy, Terbuthylazine-desethyl,
• MeSH
• antioxidancia metabolismus   MeSH
• atrazin metabolismus   toxicita   MeSH
• chemické látky znečišťující vodu metabolismus   toxicita   MeSH
• embryo nesavčí účinky léků   enzymologie   metabolismus   MeSH
• glutathion metabolismus   MeSH
• glutathionreduktasa metabolismus   MeSH
• glutathiontransferasa metabolismus   MeSH
• herbicidy metabolismus   toxicita   MeSH
• játra účinky léků   embryologie   patologie   MeSH
• kapři embryologie   metabolismus   MeSH
• katalasa metabolismus   MeSH
• látky reagující s kyselinou thiobarbiturovou metabolismus   MeSH
• oxidační stres účinky léků   MeSH
• peroxidace lipidů účinky léků   MeSH
• superoxiddismutasa metabolismus   MeSH
• triaziny metabolismus   toxicita   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antioxidancia MeSH
• atrazin MeSH
• chemické látky znečišťující vodu MeSH
• glutathion MeSH
• glutathionreduktasa MeSH
• glutathiontransferasa MeSH
• herbicidy MeSH
• katalasa MeSH
• látky reagující s kyselinou thiobarbiturovou MeSH
• superoxiddismutasa MeSH
• terbutylazine MeSH Prohlížeč
• triaziny MeSH