Control of common scab disease can be reached by resistant cultivars or suppressive soils. Both mechanisms are likely to translate into particular potato microbiome profiles, but the relative importance of each is not known. Here, microbiomes of bulk and tuberosphere soil and of potato periderm were studied in one resistant and one susceptible cultivar grown in a conducive and a suppressive field. Disease severity was suppressed similarly by both means yet, the copy numbers of txtB gene (coding for a pathogenicity determinant) were similar in both soils but higher in periderms of the susceptible cultivar from conducive soil. Illumina sequencing of 16S rRNA genes for bacteria (completed by 16S rRNA microarray approach) and archaea, and of 18S rRNA genes for micro-eukarytes showed that in bacteria, the more important was the effect of cultivar and diversity decreased from resistant cultivar to bulk soil to susceptible cultivar. The major changes occurred in proportions of Actinobacteria, Chloroflexi, and Proteobacteria. In archaea and micro-eukaryotes, differences were primarily due to the suppressive and conducive soil. The effect of soil suppressiveness × cultivar resistance depended on the microbial community considered, but differed also with respect to soil and plant nutrient contents particularly in N, S and Fe.

• MeSH
• Actinobacteria klasifikace   genetika   růst a vývoj   patogenita   MeSH
• Archaea klasifikace   genetika   růst a vývoj   patogenita   MeSH
• Chloroflexi klasifikace   genetika   růst a vývoj   patogenita   MeSH
• dusík metabolismus   farmakologie   MeSH
• eukaryotické buňky metabolismus   MeSH
• faktory virulence genetika   metabolismus   MeSH
• genotypizační techniky MeSH
• mikrobiota genetika   MeSH
• náchylnost k nemoci imunologie   MeSH
• nemoci rostlin imunologie   mikrobiologie   MeSH
• odolnost vůči nemocem účinky léků   MeSH
• Proteobacteria klasifikace   genetika   růst a vývoj   patogenita   MeSH
• půdní mikrobiologie * MeSH
• RNA ribozomální 16S genetika   MeSH
• RNA ribozomální 18S genetika   MeSH
• síra metabolismus   farmakologie   MeSH
• Solanum tuberosum účinky léků   imunologie   mikrobiologie   MeSH
• železo metabolismus   farmakologie   MeSH
• zemědělské plodiny MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• dusík MeSH
• faktory virulence MeSH
• RNA ribozomální 16S MeSH
• RNA ribozomální 18S MeSH
• síra MeSH
• železo MeSH

Common scab of potatoes is a disease, which is difficult to manage due to complex interactions of the pathogenic bacteria (Streptomyces spp.) with soil, microbial community and potato plants. In Bohemian-Moravian Highlands in the Czech Republic two sites (Vyklantice and Zdirec) were selected for a study of common scab disease suppressivity. At both sites, a field with low disease severity occurs next to one with high severity and the situation was regularly observed over four decades although all four fields undergo a crop rotation. In the four fields, quantities of bacteria, actinobacteria and the gene txtB from the biosynthetic gene cluster of thaxtomin, the main pathogenicity factor of common scab, were analyzed by real-time PCR. Microbial community structure was compared by terminal fragment length polymorphism analysis. Soil and potato periderm were characterized by contents of carbon, nitrogen, phosphorus, sulphur, calcium, magnesium, and iron. Quality of organic matter was assessed by high performance liquid chromatography of soil extracts. The study demonstrated that the suppressive character of the fields is locally specific. At Zdirec, the suppressivity was associated with low txtB gene copies in bulk soil, while at Vyklantice site it was associated with low txtB gene copies in the tuberosphere. The differences were discussed with respect to the effect of abiotic conditions at Zdirec and interaction between potato plant and soil microbial community at Vyklantice. Soil pH, Ca soil content or cation concentrations, although different were not in the range to predict the disease severity. Low severity of common scab was associated with low content of soil C, N, C/N, Ca and Fe suggesting that oligotrophic conditions may be favorable to common scab suppression.

• MeSH
• bakteriální geny * MeSH
• hlízy rostlin mikrobiologie   MeSH
• indoly metabolismus   MeSH
• multigenová rodina * MeSH
• nemoci rostlin mikrobiologie   MeSH
• piperaziny metabolismus   MeSH
• polymorfismus délky restrikčních fragmentů * MeSH
• půdní mikrobiologie * MeSH
• Solanum tuberosum mikrobiologie   MeSH
• Streptomyces genetika   metabolismus   patogenita   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• indoly MeSH
• piperaziny MeSH
• thaxtomine B MeSH Prohlížeč