The Permo-Triassic mass extinction was linked to catastrophic environmental changes and large igneous province (LIP) volcanism. In addition to the widespread marine losses, the Permo-Triassic event was the most severe terrestrial ecological crisis in Earth's history and the only known mass extinction among insects, but the cause of extinction on land remains unclear. In this study, high-resolution Hg concentration records and multiple-archive S-isotope analyses of sediments from the Junggar Basin (China) provide evidence of repeated pulses of volcanic-S (acid rain) and increased Hg loading culminating in a crisis of terrestrial biota in the Junggar Basin coeval with the interval of LIP emplacement. Minor S-isotope analyses are, however, inconsistent with total ozone layer collapse. Our data suggest that LIP volcanism repeatedly stressed end-Permian terrestrial environments in the ~300 kyr preceding the marine extinction locally via S-driven acidification and deposition of Hg, and globally via pulsed addition of CO2.

• MeSH
• extinkce biologická * MeSH
• geologické sedimenty * MeSH
• izotopy síry analýza   MeSH
• rtuť analýza   MeSH
• sopečné erupce * MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• historické články MeSH
• Geografické názvy
• Čína MeSH
• Názvy látek
• izotopy síry MeSH
• rtuť MeSH

Sulfur-oxidizing bacteria (SOB) are versatile microorganisms known for their ability to oxidize various reduced sulfur compounds, namely, elemental sulfur (S0), hydrogen sulfide (H2S), tetrathionate (S4O62-), and trithionate (S3O62-) to sulfate (SO42-). In this study, out of twelve SOB isolates from rice rhizosphere, five were screened based on their sulfur oxidation potential, viz., SOB1, SOB2, SOB3, SOB4, and SOB5, and were identified as Ochrobactrum soli SOB1, Achromobacter xylosoxidans SOB2, Stenotrophomonas maltophilia SOB3, Brucella tritici SOB4, and Stenotrophomonas pavanii SOB5, respectively. All the isolates displayed chemolithotrophic nutritional mode by consuming thiosulfate and accumulating trithionate and tetrathionate in the growth medium which is ultimately oxidized to sulfate. The strains were authenticated with the production of thiosulfate oxidizing enzymes such as rhodanese and sulfite oxidase. Despite their tendency to oxidize reduced sulfur compounds, B. tritici SOB4 and S. pavanii SOB5 were also found to possess phosphate and zinc solubilization potential, acetic acid, and indole acetic acid (IAA) production and 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity. The presence of sulfanyl (R-SH) groups was noticed in the A. xylosoxidans SOB2. Elemental sulfur conversion into sulfate was noted in the S. maltophilia SOB3, and hydrogen sulfide conversion into sulfate was observed in the Ochromobacter soli SOB1. Sulfur oxidation potential coupled with beneficial properties of the isolates widen the knowledge on SOB.

• Klíčová slova
• IAA, Phosphate solubilization, Rhodanase, Sulfate, Sulfur-oxidizing bacteria (SOB), Tetrathionate, Thiosulfate,
• MeSH
• Bacteria genetika   MeSH
• kyseliny síry * MeSH
• oxidace-redukce MeSH
• rhizosféra MeSH
• rýže (rod) * MeSH
• síra MeSH
• sírany MeSH
• sloučeniny síry MeSH
• sulfan * MeSH
• thiosírany MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• kyseliny síry * MeSH
• síra MeSH
• sírany MeSH
• sloučeniny síry MeSH
• sulfan * MeSH
• thiosírany MeSH
• trithionic acid MeSH Prohlížeč

The development of a viable sensor for agrochemical pesticides requires the assessment of trace levels. To achieve this, we developed a diphenylamine (DPA) sensor using needle-like strontium pyrophosphate embedded in sulfur-doped graphitic carbon nitride (SrPO/SCN). We obtained needle-like SrPO/SCN nanocomposite through co-precipitation followed by ultrasonication. The formation of the SrPO/SCN nanocomposite was verified through FT-IR, XRD, XPS, SEM-EDX, and HR-TEM analyses. Additionally, we explored their electrochemical behavior towards DPA using differential pulse voltammetry (DPV) and cyclic voltammetry (CV). The SrPO/SCN nanocomposite-modified electrode exhibited a higher anodic peak current (15.47 µA) than those of the other modified and unmodified electrodes. Under optimal experimental conditions, SrPO/SCN/GCE demonstrated a good limit of detection (0.009 µmol/L), dynamic linear range (0.05-98 µmol/L), and sensitivity (0.36 µAµM-1cm-2). Furthermore, the developed sensor exhibited excellent reproducibility, selectivity, and stability, and successfully detected DPA in real samples, including pear and apple samples, with good recoveries.

• Klíčová slova
• Diphenylamine, Electrochemical sensor, Metal Pyrophosphate, Nanocomposite, Real-time analysis,
• MeSH
• agrochemikálie MeSH
• difosfáty MeSH
• elektrochemické techniky MeSH
• elektrody MeSH
• pesticidy * MeSH
• reprodukovatelnost výsledků MeSH
• síra MeSH
• spektroskopie infračervená s Fourierovou transformací MeSH
• uhlík MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• agrochemikálie MeSH
• difosfáty MeSH
• graphitic carbon nitride MeSH Prohlížeč
• pesticidy * MeSH
• síra MeSH
• uhlík MeSH

Sulfur (S) is an essential mineral nutrient for plant growth and development; it is important for primary and specialized plant metabolites that are crucial for biotic and abiotic interactions. Foliar S content varies up to 6-fold under a controlled environment, suggesting an adaptive value under certain natural environmental conditions. However, a major quantitative regulator of S content in Arabidopsis thaliana has not been identified yet, pointing to the existence of either additional genetic factors controlling sulfate/S content or of many minor quantitative regulators. Here, we use overlapping information of two separate ionomics studies to select groups of accessions with low, mid, and high foliar S content. We quantify series of metabolites, including anions (sulfate, phosphate, and nitrate), thiols (cysteine and glutathione), and seven glucosinolates, gene expression of 20 genes, sulfate uptake, and three biotic traits. Our results suggest that S content is tightly connected with sulfate uptake, the concentration of sulfate and phosphate anions, and glucosinolate and glutathione synthesis. Additionally, our results indicate that the growth of pathogenic bacteria is enhanced in the A. thaliana accessions containing higher S in their leaves, suggesting a complex regulation between S homeostasis, primary and secondary metabolism, and biotic pressures.

• Klíčová slova
• Arabidopsis thaliana, gene expression, glucosinolates, glutathione, natural variation, nutrients, sulfur,
• MeSH
• anionty metabolismus   MeSH
• Arabidopsis * metabolismus   MeSH
• fosfáty metabolismus   MeSH
• glukosinoláty MeSH
• glutathion metabolismus   MeSH
• proteiny huseníčku * metabolismus   MeSH
• regulace genové exprese u rostlin MeSH
• síra metabolismus   MeSH
• sírany metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• anionty MeSH
• fosfáty MeSH
• glukosinoláty MeSH
• glutathion MeSH
• proteiny huseníčku * MeSH
• síra MeSH
• sírany MeSH

Illiosentis Van Cleave et Lincicome, 1939 initially included two species: Illiosentis furcatus Van Cleave et Lincicome, 1939 found in the West Atlantic from Cape Cod in Massachusetts, USA to northern Argentina and Illiosentis cetratus Van Cleave, 1945 with restricted distribution in the Pacific coast of southern California. We are reporting I. furcatus from Peru for the first time and describe a population of I. cetratus from the California corbina, Menticirrhus undulatus (Girard), from southern California. The proboscis hook formula was 14 longitudinal rows for I. furcatus of 18-23 hooks each compared to 16 rows of 19-24 hooks each reported by Van Cleave (1945). We complete the inadequate description of I. cetratus with new information on sexual differentiation in the length of the trunk, dorsal vs. ventral hooks, hook roots, trunk spines, two types of anterior recurved rooted hooks vs. posterior rootless straight hooks, measurements of dorsal and ventral hooks and spines, shape of hook roots, terminal position of the female gonopore, and of position of the cephalic ganglion at the anterior margin of the trunk. We also include new details of the reproductive system in both sexes including Saefftigen's pouch and cement gland ducts. We present new SEM and light microscope images. The Energy Dispersive X-ray analysis (EDXA) shows a high level of sulfur in anterior, middle and posterior hooks in various hook sites, as well as spectra of hook tips with a higher relative concentration of sulfur compared to other hook sites. For the placement of I. cetratus, phylogenetic analysis of sequences of three molecular markers, 18S, 28S rRNA and mitochondrial cox 1 genes, was performed with other related available sequences. The resulting analysis illustrated that I. cetratus was nested within a separate clade along with species of two genera, Dentitruncus truttae Sinzar, 1955 and Neotegorhynchus cyprini Lisitsyna, Xi, Orosová, Barčák et Oros, 2022 represented our species of Illiosentis separate from species of Tegorhynchus Van Cleave, 1921 (as also according to the morphology) with which the Illiosentis species were previously synonymised.

• Klíčová slova
• California corbina, EDXA, SEM, molecular profile, redescription,
• MeSH
• Acanthocephala * anatomie a histologie   MeSH
• fylogeneze MeSH
• helmintózy zvířat * epidemiologie   MeSH
• nemoci ryb * epidemiologie   MeSH
• Perciformes * MeSH
• síra MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Peru MeSH
• Názvy látek
• síra MeSH

The recent revision of the Acidithiobacillia class using genomic taxonomy methods has shown that, in addition to the existence of previously unrecognized genera and species, some species of the class harbor levels of divergence that are congruent with ongoing differentiation processes. In this study, we have performed a subspecies-level analysis of sequenced strains of Acidithiobacillus ferrooxidans to prove the existence of distinct sublineages and identify the discriminant genomic/genetic characteristics linked to these sublineages, and to shed light on the processes driving such differentiation. Differences in the genomic relatedness metrics, levels of synteny, gene content, and both integrated and episomal mobile genetic elements (MGE) repertoires support the existence of two subspecies-level taxa within A. ferrooxidans. While sublineage 2A harbors a small plasmid related to pTF5, this episomal MGE is absent in sublineage 2B strains. Likewise, clear differences in the occurrence, coverage and conservation of integrated MGEs are apparent between sublineages. Differential MGE-associated gene cargo pertained to the functional categories of energy metabolism, ion transport, cell surface modification, and defense mechanisms. Inferred functional differences have the potential to impact long-term adaptive processes and may underpin the basis of the subspecies-level differentiation uncovered within A. ferrooxidans. Genome resequencing of iron- and sulfur-adapted cultures of a selected 2A sublineage strain (CCM 4253) showed that both episomal and large integrated MGEs are conserved over twenty generations in either growth condition. In turn, active insertion sequences profoundly impact short-term adaptive processes. The ISAfe1 element was found to be highly active in sublineage 2A strain CCM 4253. Phenotypic mutations caused by the transposition of ISAfe1 into the pstC2 encoding phosphate-transport system permease protein were detected in sulfur-adapted cultures and shown to impair growth on ferrous iron upon the switch of electron donor. The phenotypic manifestation of the △pstC2 mutation, such as a loss of the ability to oxidize ferrous iron, is likely related to the inability of the mutant to secure the phosphorous availability for electron transport-linked phosphorylation coupled to iron oxidation. Depletion of the transpositional △pstC2 mutation occurred concomitantly with a shortening of the iron-oxidation lag phase at later transfers on a ferrous iron-containing medium. Therefore, the pstII operon appears to play an essential role in A. ferrooxidans when cells oxidize ferrous iron. Results highlight the influence of insertion sequences and both integrated and episomal mobile genetic elements in the short- and long-term adaptive processes of A. ferrooxidans strains under changing growth conditions.

• MeSH
• Acidithiobacillus * genetika   metabolismus   MeSH
• oxidace-redukce MeSH
• síra metabolismus   MeSH
• transpozibilní elementy DNA * genetika   MeSH
• železo metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• síra MeSH
• transpozibilní elementy DNA * MeSH
• železo MeSH

Climatic conditions affect animals but range-wide impacts at the population level remain largely unknown, especially in migratory species. However, studying climate-population relationships is still challenging in small migrants due to a lack of efficient and cost-effective geographic tracking method. Spatial distribution patterns of environmental stable isotopes (so called 'isoscapes') generally overcome these limitations but none of the currently available isoscapes provide a substantial longitudinal gradient in species-rich sub-Saharan Africa. In this region, sulphur (δ34 S) has not been sufficiently explored on a larger scale. We developed a δ34 S isoscape to trace animal origins in sub-Saharan Africa by coupling known-origin samples from tracked migratory birds with continental remotely sensed environmental data building on environment-δ34 S relationships using a flexible machine learning technique. Furthermore, we link population-specific nonbreeding grounds with interannual climatic variation that might translate to breeding population trends. The predicted δ34 S isotopic map featured east-west and coast-to-inland isotopic gradients and was applied to predict nonbreeding grounds of three breeding populations of Eurasian Reed Warblers Acrocephalus scirpaceus with two distinct migratory phenotypes. Breeding populations as well as migratory phenotypes exhibited large-scale segregation within the African nonbreeding range. These regions also differed substantially in the interannual climatic variation, with higher interannual variability in the eastern part of the range during 2001-2012. Over the same period, the eastern European breeding population seemed to have experienced a more steep decline in population size. The link between migratory patterns and large-scale climatic variability appears important to better understand population trajectories in many declining migratory animals. We believe animal tracing using sulphur isotopes will facilitate these efforts and offers manifold ecological and forensic applications in the biodiversity hotspot of sub-Saharan Africa.

• Klíčová slova
• climate variability, isoscape, migration, migratory divide, remote sensing, sulphur, tracing, tracking,
• MeSH
• hustota populace MeSH
• izotopy síry MeSH
• migrace zvířat MeSH
• roční období MeSH
• zpěvní ptáci * MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, U.S. Gov't, Non-P.H.S. MeSH
• Geografické názvy
• Afrika MeSH
• Názvy látek
• izotopy síry MeSH

Sulfide intrusion from sediments is an increasingly recognized contributor to seagrass declines globally, yet the relationship between sediment microorganisms and sulfide intrusion has received little attention. Here, we use metagenomic sequencing and stable isotope (34S) analysis to examine this relationship in Cockburn Sound, Australia, a seagrass-dominated embayment with a gradient of sulfide stress and seagrass declines. There was a significant positive relationship between sulfide intrusion into seagrasses and sulfate reduction genes in sediment microbial communities, which was greatest at sites with long term seagrass declines. This is the first demonstration of a significant link between sulfur cycling genes present in seagrass sediments and sulfide intrusion in a habitat-forming seagrass that is experiencing long-term shoot density decline. Given that microorganisms respond rapidly to environmental change, the quantitative links established in this study can be used as a potential management tool to enable the prediction of sulfide stress on large habitat forming seagrasses; a global issue expected to worsen with climate change.

• Klíčová slova
• Coastal, Conservation, Macrophyte, Metagenomics, Stable isotope, posidonia,
• MeSH
• ekosystém MeSH
• geologické sedimenty * MeSH
• mikrobiota * MeSH
• síra MeSH
• sulfidy MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Austrálie MeSH
• Názvy látek
• síra MeSH
• sulfidy MeSH

A chemoselective procedure for MCPBA oxidation of 26-thiodiosgenin to corresponding sulfoxides and sulfone was elaborated. An unusual equilibration of sulfoxides in solution was observed. Moreover, α-alkylation of sulfoxide and sulfone was investigated. Finally, the biological activity of obtained compounds was examined.

• Klíčová slova
• 26-thiodiosgenin, antimicrobial activity, cytotoxicity, sapogenins, steroids, sulfones, sulfoxides, thiol oxidation,
• MeSH
• diosgenin * MeSH
• oxidace-redukce MeSH
• síra chemie   MeSH
• sulfony MeSH
• sulfoxidy chemie   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• diosgenin * MeSH
• síra MeSH
• sulfony MeSH
• sulfoxidy MeSH

The phytohormone cytokinin is implicated in a range of growth, developmental, and defense processes. A growing body of evidence supports a crosstalk between cytokinin and nutrient signaling pathways, such as nitrate availability. Cytokinin signaling regulates sulfur-responsive gene expression, but the underlying molecular mechanisms and their impact on sulfur-containing metabolites have not been systematically explored. Using a combination of genetic and pharmacological tools, we investigated the interplay between cytokinin signaling and sulfur homeostasis. Exogenous cytokinin triggered sulfur starvation-like gene expression accompanied by a decrease in sulfate and glutathione content. This process was uncoupled from the activity of the major transcriptional regulator of sulfate starvation signaling SULFUR LIMITATION 1 and an important glutathione-degrading enzyme, γ-glutamyl cyclotransferase 2;1, expression of which was robustly up-regulated by cytokinin. Conversely, glutathione accumulation was observed in mutants lacking the cytokinin receptor ARABIDOPSIS HISTIDINE KINASE 3 and in cytokinin-deficient plants. Cytokinin-deficient plants displayed improved root growth upon exposure to glutathione-depleting chemicals which was attributed to a higher capacity to maintain glutathione levels. These results shed new light on the interplay between cytokinin signaling and sulfur homeostasis. They position cytokinin as an important modulator of sulfur uptake, assimilation, and remobilization in plant defense against xenobiotics and root growth.

• Klíčová slova
• Arabidopsis thaliana, cytokinin, glutathione, nutrient homeostasis, sulfur metabolism, sulfur starvation, xenobiotic tolerance,
• MeSH
• cytokininy * MeSH
• glutathion MeSH
• metabolické sítě a dráhy MeSH
• síra * MeSH
• sírany MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• cytokininy * MeSH
• glutathion MeSH
• síra * MeSH
• sírany MeSH