Energetic ions represent an important tool for the creation of controlled structural defects in solid nanomaterials. However, the current preparative irradiation techniques in accelerators show significant limitations in scaling-up, because only very thin layers of nanoparticles can be efficiently and homogeneously irradiated. Here, we show an easily scalable method for rapid irradiation of nanomaterials by light ions formed homogeneously in situ by a nuclear reaction. The target nanoparticles are embedded in B2O3 and placed in a neutron flux. Neutrons captured by 10B generate an isotropic flux of energetic α particles and 7Li+ ions that uniformly irradiates the surrounding nanoparticles. We produced 70 g of fluorescent nanodiamonds in an approximately 30-minute irradiation session, as well as fluorescent silicon carbide nanoparticles. Our method thus increased current preparative yields by a factor of 102-103. We envision that our technique will increase the production of ion-irradiated nanoparticles, facilitating their use in various applications.

• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Development of multifunctional nanoscale sensors working under physiological conditions enables monitoring of intracellular processes that are important for various biological and medical applications. By attaching paramagnetic gadolinium complexes to nanodiamonds (NDs) with nitrogen-vacancy (NV) centres through surface engineering, we developed a hybrid nanoscale sensor that can be adjusted to directly monitor physiological species through a proposed sensing scheme based on NV spin relaxometry. We adopt a single-step method to measure spin relaxation rates enabling time-dependent measurements on changes in pH or redox potential at a submicrometre-length scale in a microfluidic channel that mimics cellular environments. Our experimental data are reproduced by numerical simulations of the NV spin interaction with gadolinium complexes covering the NDs. Considering the versatile engineering options provided by polymer chemistry, the underlying mechanism can be expanded to detect a variety of physiologically relevant species and variables.

• MeSH
• biosenzitivní techniky metody   MeSH
• časové faktory MeSH
• koncentrace vodíkových iontů MeSH
• konfokální mikroskopie MeSH
• kvantová teorie MeSH
• nanodiamanty chemie   ultrastruktura   MeSH
• nanotechnologie metody   MeSH
• optické zobrazování metody   MeSH
• oxidace-redukce MeSH
• reprodukovatelnost výsledků MeSH
• transmisní elektronová mikroskopie MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• nanodiamanty MeSH

Targeted biocompatible nanostructures with controlled plasmonic and morphological parameters are promising materials for cancer treatment based on selective thermal ablation of cells. Here, core-shell plasmonic nanodiamonds consisting of a silica-encapsulated diamond nanocrystal coated in a gold shell are designed and synthesized. The architecture of particles is analyzed and confirmed in detail using electron tomography. The particles are biocompatibilized using a PEG polymer terminated with bioorthogonally reactive alkyne groups. Azide-modified transferrin is attached to these particles, and their high colloidal stability and successful targeting to cancer cells overexpressing the transferrin receptor are demonstrated. The particles are nontoxic to the cells and they are readily internalized upon binding to the transferrin receptor. The high plasmonic cross section of the particles in the near-infrared region is utilized to quantitatively ablate the cancer cells with a short, one-minute irradiation by a pulse 750-nm laser.

• Klíčová slova
• ablation, cancer, gold, nanodiamonds, plasmonics,
• MeSH
• ablace metody   MeSH
• biokompatibilní materiály farmakokinetika   MeSH
• cílená molekulární terapie metody   MeSH
• HeLa buňky účinky léků   MeSH
• indukovaná hypertermie metody   MeSH
• karbocyaniny chemie   MeSH
• laserová terapie metody   MeSH
• lidé MeSH
• nanočástice chemie   MeSH
• nanodiamanty chemie   MeSH
• nanoslupky chemie   MeSH
• polyethylenglykoly chemie   MeSH
• receptory transferinu metabolismus   MeSH
• transferin chemie   farmakologie   MeSH
• zlato chemie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, N.I.H., Extramural MeSH
• Názvy látek
• Alexa Fluor 647 MeSH Prohlížeč
• biokompatibilní materiály MeSH
• karbocyaniny MeSH
• nanodiamanty MeSH
• polyethylenglykoly MeSH
• receptory transferinu MeSH
• transferin MeSH
• zlato MeSH