Previously reported DNA aptamers developed against surface proteins extracted from Campylobacter jejuni were further characterized by aptamer-based Western blotting and shown to bind epitopes on proteins weighing ~16 and 60 kD from reduced C. jejuni and Campylobacter coli lysates. Proteins of these approximate weights have also been identified in traditional antibody-based Western blots of Campylobacter spp. Specificity of the capture and reporter aptamers from the previous report was further validated by aptamer-based ELISA-like (ELASA) colorimetric microplate assay. Finally, the limit of detection of the previously reported plastic-adherent aptamer-magnetic bead and aptamer-quantum dot sandwich assay (PASA) was validated by an independent food safety testing laboratory to lie between 5 and 10 C. jejuni cells per milliliter in phosphate buffered saline and repeatedly frozen and thawed chicken rinsate. Such ultrasensitive and rapid (30 min) aptamer-based assays could provide alternative or additional screening tools to enhance food safety testing for Campylobacter and other foodborne pathogens.

• Klíčová slova
• External Magnet, Foodborne Pathogen, Sandwich Assay, Small Detection Area, Sterile Buffer,
• MeSH
• aptamery nukleotidové genetika   MeSH
• biotest přístrojové vybavení   metody   MeSH
• Campylobacter genetika   izolace a purifikace   MeSH
• kontaminace potravin analýza   MeSH
• kur domácí MeSH
• kvantové tečky chemie   MeSH
• magnetismus metody   MeSH
• maso mikrobiologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• validační studie MeSH
• Názvy látek
• aptamery nukleotidové MeSH