Honey consumption is attributed to potentially advantageous effects on human health due to its antioxidant capacity as well as anti-inflammatory and antimicrobial activity, which are mainly related to phenolic compound content. Phenolic compounds are secondary metabolites of plants, and their content in honey is primarily affected by the botanical and geographical origin. In this study, a high-resolution mass spectrometry (HRMS) method was applied to determine the phenolic profile of various honey matrices and investigate authenticity markers. A fruitful sample set was collected, including honey from 10 different botanical sources (n = 51) originating from Greece and Poland. Generic liquid-liquid extraction using ethyl acetate as the extractant was used to apply targeted and non-targeted workflows simultaneously. The method was fully validated according to the Eurachem guidelines, and it demonstrated high accuracy, precision, and sensitivity resulting in the detection of 11 target analytes in the samples. Suspect screening identified 16 bioactive compounds in at least one sample, with abscisic acid isomers being the most abundant in arbutus honey. Importantly, 10 markers related to honey geographical origin were revealed through non-targeted screening and the application of advanced chemometric tools. In conclusion, authenticity markers and discrimination patterns were emerged using targeted and non-targeted workflows, indicating the impact of this study on food authenticity and metabolomic fields.

• Klíčová slova
• UPLC–QToF-MS, authenticity, geographical origin, honey, metabolomics, origin discrimination, phenolic compounds,
• MeSH
• antioxidancia analýza   izolace a purifikace   MeSH
• benzaldehydy analýza   izolace a purifikace   MeSH
• cinnamáty analýza   izolace a purifikace   MeSH
• flavonoidy analýza   izolace a purifikace   MeSH
• hmotnostní spektrometrie metody   MeSH
• hydroxybenzoáty analýza   izolace a purifikace   MeSH
• lidé MeSH
• med analýza   MeSH
• metabolom * MeSH
• metabolomika metody   MeSH
• senzitivita a specificita MeSH
• správnost dat MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• validační studie MeSH
• Geografické názvy
• Polsko MeSH
• Řecko MeSH
• Názvy látek
• antioxidancia MeSH
• benzaldehydy MeSH
• cinnamáty MeSH
• flavonoidy MeSH
• hydroxybenzoáty MeSH
• vanillin MeSH Prohlížeč

Honey is a high-value, globally consumed, food product featuring a high market price strictly related to its origin. Moreover, honey origin has to be clearly stated on the label, and quality schemes are prescribed based on its geographical and botanical origin. Therefore, to enhance food quality, it is of utmost importance to develop analytical methods able to accurately and precisely discriminate honey origin. In this study, an all-time scientometric evaluation of the field is provided for the first time using a structured keyword on the Scopus database. The bibliometric analysis pinpoints that the botanical origin discrimination was the most studied authenticity issue, and chromatographic methods were the most frequently used for its assessment. Based on these results, we comprehensively reviewed analytical techniques that have been used in honey authenticity studies. Analytical breakthroughs and bottlenecks on methodologies to assess honey quality parameters using separation, bioanalytical, spectroscopic, elemental and isotopic techniques are presented. Emphasis is given to authenticity markers, and the necessity to apply chemometric tools to reveal them. Altogether, honey authenticity is an ever-growing field, and more advances are expected that will further secure honey quality.

• Publikační typ
• časopisecké články MeSH
• přehledy MeSH