The translation of metallothioneins (MTs) is one of the defense strategies by which organisms protect themselves from metal-induced toxicity. MTs belong to a family of proteins comprising MT-1, MT-2, MT-3, and MT-4 classes, with multiple isoforms within each class. The main aim of this study was to determine the behavior of MT in dependence on various externally modelled environments, using electrochemistry. In our study, the mass distribution of MTs was characterized using MALDI-TOF. After that, adsorptive transfer stripping technique with differential pulse voltammetry was selected for optimization of electrochemical detection of MTs with regard to accumulation time and pH effects. Our results show that utilization of 0.5 M NaCl, pH 6.4, as the supporting electrolyte provides a highly complicated fingerprint, showing a number of non-resolved voltammograms. Hence, we further resolved the voltammograms exhibiting the broad and overlapping signals using curve fitting. The separated signals were assigned to the electrochemical responses of several MT complexes with zinc(II), cadmium(II), and copper(II), respectively. Our results show that electrochemistry could serve as a great tool for metalloproteomic applications to determine the ratio of metal ion bonds within the target protein structure, however, it provides highly complicated signals, which require further resolution using a proper statistical method, such as curve fitting.

• Klíčová slova
• electrochemistry, mass spectrometry MALDI-TOF, metallomics, metallothionein, signal resolving,
• MeSH
• chlorid sodný chemie   MeSH
• elektrochemie MeSH
• elektrolyty MeSH
• komplexní sloučeniny chemie   metabolismus   MeSH
• kovy chemie   metabolismus   MeSH
• metalothionein chemie   metabolismus   MeSH
• protein - isoformy MeSH
• spektrometrie hmotnostní - ionizace laserem za účasti matrice MeSH
• vazba proteinů MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• chlorid sodný MeSH
• elektrolyty MeSH
• komplexní sloučeniny MeSH
• kovy MeSH
• metalothionein MeSH
• protein - isoformy MeSH

CdTe/ZnSe core/shell quantum dot (QD), one of the strongest and most highly luminescent nanoparticles, was directly synthesized in an aqueous medium to study its individual interactions with important nucleobases (adenine, guanine, cytosine, and thymine) in detail. The results obtained from the optical analyses indicated that the interactions of the QDs with different nucleobases were different, which reflected in different fluorescent emission maxima and intensities. The difference in the interaction was found due to the different chemical behavior and different sizes of the formed nanoconjugates. An electrochemical study also confirmed that the purines and pyrimidines show different interactions with the core/shell QDs. Based on these phenomena, a novel QD-based method is developed to detect the presence of the DNA, damage to DNA, and mutation. The QDs were successfully applied very easily to detect any change in the sequence (mutation) of DNA. The QDs also showed their ability to detect DNAs directly from the extracts of human cancer (PC3) and normal (PNT1A) cells (detection limit of 500 pM of DNA), which indicates the possibilities to use this easy assay technique to confirm the presence of living organisms in extreme environments.

• Klíčová slova
• biosensor, fluorescence, mutation, nanoparticles, nucleobases,
• MeSH
• DNA analýza   MeSH
• dynamický rozptyl světla MeSH
• elektrochemické techniky MeSH
• kvantové tečky chemie   ultrastruktura   MeSH
• lidé MeSH
• mutace genetika   MeSH
• nádorové buněčné linie MeSH
• poškození DNA * MeSH
• sloučeniny kadmia chemie   MeSH
• sloučeniny selenu chemie   MeSH
• sloučeniny zinku chemie   MeSH
• spektroskopie infračervená s Fourierovou transformací MeSH
• statická elektřina MeSH
• telur chemie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• cadmium telluride MeSH Prohlížeč
• DNA MeSH
• sloučeniny kadmia MeSH
• sloučeniny selenu MeSH
• sloučeniny zinku MeSH
• telur MeSH
• zinc selenide MeSH Prohlížeč

Herein, we describe the preparation of liposomes with folate-targeting properties for the encapsulation of anti-sarcosine antibodies (antisarAbs@LIP) and sarcosine (sar@LIP). The competitive inhibitory effects of exogenously added folic acid supported the role of folate targeting in liposome internalization. We examined the effects of repeated administration on mice PC-3 xenografts. Sar@LIP treatment significantly increased tumor volume and weight compared to controls treated with empty liposomes. Moreover, antisarAbs@LIP administration exhibited a mild antitumor effect. We also identified differences in gene expression patterns post-treatment. Furthermore, Sar@LIP treatment resulted in decreased amounts of tumor zinc ions and total metallothioneins. Examination of the spatial distribution across the tumor sections revealed a sarcosine-related decline of the MT1X isoform within the marginal regions but an elevation after antisarAbs@LIP administration. Our exploratory results demonstrate the importance of sarcosine as an oncometabolite in PCa. Moreover, we have shown that sarcosine can be a potential target for anticancer strategies in management of PCa.

• MeSH
• biologické modely MeSH
• fosfatidylethanolaminy MeSH
• kyselina listová metabolismus   MeSH
• lidé MeSH
• liposomy * chemie   ultrastruktura   MeSH
• metalothionein metabolismus   MeSH
• modely nemocí na zvířatech MeSH
• monoklonální protilátky aplikace a dávkování   MeSH
• myši MeSH
• nádorové buněčné linie MeSH
• nádory prostaty farmakoterapie   metabolismus   patologie   MeSH
• sarkosin antagonisté a inhibitory   chemie   MeSH
• tumor burden účinky léků   MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• xenogenní modely - testy protinádorové aktivity MeSH
• zinek metabolismus   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• dioleoyl phosphatidylethanolamine MeSH Prohlížeč
• fosfatidylethanolaminy MeSH
• kyselina listová MeSH
• liposomy * MeSH
• metalothionein MeSH
• monoklonální protilátky MeSH
• sarkosin MeSH
• zinek MeSH