Nejvíce citovaný článek - PubMed ID 26445407
Determinants encoding fimbriae type 1 in fecal Escherichia coli are associated with increased frequency of bacteriocinogeny
Escherichia albertii is a recently discovered species with a limited number of well characterized strains. The aim of this study was to characterize four of the E. albertii strains, which were among 41 identified Escherichia strains isolated from the feces of living animals on James Ross Island, Antarctica, and Isla Magdalena, Patagonia. Sequencing of 16S rDNA, automated ribotyping, and rep-PCR were used to identify the four E. albertii isolates. Phylogenetic analyses based on multi-locus sequence typing showed these isolates to be genetically most similar to the members of E. albertii phylogroup G3. These isolates encoded several virulence factors including those, which are characteristic of E. albertii (cytolethal distending toxin and intimin) as well as bacteriocin determinants that typically have a very low prevalence in E. coli strains (D, E7). Moreover, E. albertii protein extracts caused cell cycle arrest in human cell line A375, probably because of cytolethal distending toxin activity.
- Klíčová slova
- Antarctica, Escherichia albertii, bacteriocins, cytolethal distending toxin,
- MeSH
- Charadriiformes mikrobiologie MeSH
- Escherichia genetika izolace a purifikace metabolismus MeSH
- feces mikrobiologie MeSH
- multilokusová sekvenční typizace veterinární MeSH
- polymerázová řetězová reakce veterinární MeSH
- pulzní gelová elektroforéza veterinární MeSH
- ribotypizace veterinární MeSH
- RNA ribozomální 16S genetika MeSH
- Spheniscidae mikrobiologie MeSH
- tuleňovití mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Antarktida MeSH
- Chile MeSH
- Názvy látek
- RNA ribozomální 16S MeSH