Escherichia albertii is a recently discovered species with a limited number of well characterized strains. The aim of this study was to characterize four of the E. albertii strains, which were among 41 identified Escherichia strains isolated from the feces of living animals on James Ross Island, Antarctica, and Isla Magdalena, Patagonia. Sequencing of 16S rDNA, automated ribotyping, and rep-PCR were used to identify the four E. albertii isolates. Phylogenetic analyses based on multi-locus sequence typing showed these isolates to be genetically most similar to the members of E. albertii phylogroup G3. These isolates encoded several virulence factors including those, which are characteristic of E. albertii (cytolethal distending toxin and intimin) as well as bacteriocin determinants that typically have a very low prevalence in E. coli strains (D, E7). Moreover, E. albertii protein extracts caused cell cycle arrest in human cell line A375, probably because of cytolethal distending toxin activity.

Czech Collection of Microorganisms Department of Experimental Biology Faculty of Science Masaryk University 625 00 Brno Czech Republic

Department of Biology Faculty of Medicine Masaryk University 625 00 Brno Czech Republic

Research Centre for Toxic Compounds in the Environment Faculty of Science Masaryk University 625 00 Brno Czech Republic

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Albert M. J., Alam K., Islam M., Montanaro J., Rahaman A. S., Haider K., Hossain M. A., Kibriya A. K., Tzipori S.1991. PubMed PMC

Asoshima N., Matsuda M., Shigemura K., Honda M., Yoshida H., Oda T., Hiwaki H.2015. Isolation of PubMed DOI

Asoshima N., Matsuda M., Shigemura K., Honda M., Yoshida H., Hiwaki H., Ogata K., Oda T.2014. Identification of PubMed DOI

Bezine E., Vignard J., Mirey G.2014. The cytolethal distending toxin effects on Mammalian cells: a DNA damage perspective. PubMed DOI PMC

Birosová E., Siegfried L., Kmet’ová M., Makara A., Ostró A., Gresová A., Urdzík P., Liptáková A., Molokácová M., Bártl R., Valanský L.2004. Detection of virulence factors in alpha-haemolytic PubMed DOI

Brandal L. T., Tunsjø H. S., Ranheim T. E., Løbersli I., Lange H., Wester A. L.2015. Shiga toxin 2a in PubMed DOI PMC

Budič M., Rijavec M., Petkovšek Z., Zgur-Bertok D.2011. PubMed DOI PMC

Comayras C., Tasca C., Pérès S. Y., Ducommun B., Oswald E., De Rycke J.1997. PubMed PMC

Felföldi T., Heéger Z., Vargha M., Márialigeti K.2010. Detection of potentially pathogenic bacteria in the drinking water distribution system of a hospital in Hungary. PubMed DOI

Fiedoruk K., Daniluk T., Swiecicka I., Murawska E., Sciepuk M., Leszczynska K.2014. First Complete Genome Sequence of PubMed DOI PMC

Gillor O., Giladi I., Riley M. A.2009. Persistence of colicinogenic PubMed DOI PMC

Gordon D. M., O’Brien C. L.2006. Bacteriocin diversity and the frequency of multiple bacteriocin production in PubMed DOI

Hinenoya A., Yasuda N., Hibino T., Shima A., Nagita A., Tsukamoto T., Yamasaki S.2017. Isolation and Characterization of an PubMed DOI

Hinenoya A., Shima K., Asakura M., Nishimura K., Tsukamoto T., Ooka T., Hayashi T., Ramamurthy T., Faruque S. M., Yamasaki S.2014. Molecular characterization of cytolethal distending toxin gene-positive PubMed DOI PMC

Huys G., Cnockaert M., Janda J. M., Swings J.2003. PubMed DOI

Hyma K. E., Lacher D. W., Nelson A. M., Bumbaugh A. C., Janda J. M., Strockbine N. A., Young V. B., Whittam T. S.2005. Evolutionary genetics of a new pathogenic PubMed DOI PMC

Inglis T. J. J., Merritt A. J., Bzdyl N., Lansley S., Urosevic M. N.2015. First bacteraemic human infection with PubMed DOI PMC

Kohoutová D., Šmajs D., Morávková P., Cyrany J., Morávková M., Forstlová M., Cihák M., Rejchrt S., Bureš J.2014. PubMed DOI PMC

Kuhnert P., Hacker J., Mühldorfer I., Burnens A. P., Nicolet J., Frey J.1997. Detection system for PubMed PMC

La Ragione R. M., McLaren I. M., Foster G., Cooley W. A., Woodward M. J.2002. Phenotypic and genotypic characterization of avian PubMed DOI PMC

Lindsey R. L., Fedorka-Cray P. J., Abley M., Turpin J. B., Meinersmann R. J.2015. Evaluating the occurrence of PubMed DOI PMC

López-Saucedo C., Cerna J. F., Villegas-Sepulveda N., Thompson R., Velazquez F. R., Torres J., Tarr P. I., Estrada-García T.2003. Single multiplex polymerase chain reaction to detect diverse loci associated with diarrheagenic PubMed DOI PMC

Luo C., Walk S. T., Gordon D. M., Feldgarden M., Tiedje J. M., Konstantinidis K. T.2011. Genome sequencing of environmental PubMed DOI PMC

Maeda E., Murakami K., Sera N., Ito K., Fujimoto S.2015. Detection of PubMed DOI PMC

Maheux A. F., Boudreau D. K., Bergeron M. G., Rodriguez M. J.2014. Characterization of PubMed DOI

Martínez J. L., Herrero M., de Lorenzo V.1994. The organization of intercistronic regions of the aerobactin operon of pColV-K30 may account for the differential expression of the PubMed DOI

Micenková L., Bosák J., Vrba M., Ševčíková A., Šmajs D.2016. Human extraintestinal pathogenic PubMed DOI PMC

Micenková L., Beňová A., Frankovičová L., Bosák J., Vrba M., Ševčíková A., Kmeťová M., Šmajs D.2017. Human PubMed DOI

Micenková L., Štaudová B., Bosák J., Mikalová L., Littnerová S., Vrba M., Ševčíková A., Woznicová V., Šmajs D.2014. Bacteriocin-encoding genes and ExPEC virulence determinants are associated in human fecal PubMed DOI PMC

Micenková L., Bosák J., Štaudová B., Kohoutová D., Čejková D., Woznicová V., Vrba M., Ševčíková A., Bureš J., Šmajs D.2016. Microcin determinants are associated with B2 phylogroup of human fecal PubMed DOI PMC

Morato E. P., Leomil L., Beutin L., Krause G., Moura R. A., Pestana de Castro A. F.2009. Domestic cats constitute a natural reservoir of human enteropathogenic PubMed DOI

Nimri L. F.2013. PubMed DOI

Oaks J. L., Besser T. E., Walk S. T., Gordon D. M., Beckmen K. B., Burek K. A., Haldorson G. J., Bradway D. S., Ouellette L., Rurangirwa F. R., Davis M. A., Dobbin G., Whittam T. S.2010. PubMed DOI PMC

Oh J. Y., Kang M. S., Hwang H. T., An B. K., Kwon J. H., Kwon Y. K.2011. Epidemiological investigation of PubMed DOI

Ooka T., Tokuoka E., Furukawa M., Nagamura T., Ogura Y., Arisawa K., Harada S., Hayashi T.2013. Human gastroenteritis outbreak associated with PubMed DOI PMC

Ooka T., Seto K., Kawano K., Kobayashi H., Etoh Y., Ichihara S., Kaneko A., Isobe J., Yamaguchi K., Horikawa K., Gomes T. A. T., Linden A., Bardiau M., Mainil J. G., Beutin L., Ogura Y., Hayashi T.2012. Clinical significance of PubMed DOI PMC

Ooka T., Ogura Y., Katsura K., Seto K., Kobayashi H., Kawano K., Tokuoka E., Furukawa M., Harada S., Yoshino S., Seto J., Ikeda T., Yamaguchi K., Murase K., Gotoh Y., Imuta N., Nishi J., Gomes T. A., Beutin L., Hayashi T.2015. Defining the Genome Features of PubMed PMC

Paciorek J.2002. Virulence properties of PubMed DOI

Paton A. W., Paton J. C.1998. Detection and characterization of Shiga toxigenic PubMed PMC

Pickett C. L., Cottle D. L., Pesci E. C., Bikah G.1994. Cloning, sequencing, and expression of the PubMed PMC

Pontes D. S., Pinheiro F. A., Lima-Bittencourt C. I., Guedes R. L. M., Cursino L., Barbosa F., Santos F. R., Chartone-Souza E., Nascimento A. M. A.2009. Multiple antimicrobial resistance of gram-negative bacteria from natural oligotrophic lakes under distinct anthropogenic influence in a tropical region. PubMed DOI

Rahman M. Z., Akter S., Azmuda N., Sultana M., Weill F. X., Khan S. I., Grimont P. A. D., Birkeland N. K.2013. Serological cross-reaction between O-antigens of PubMed DOI

Schmidt H., Knop C., Franke S., Aleksic S., Heesemann J., Karch H.1995. Development of PCR for screening of enteroaggregative Escherichia coli. PubMed PMC

Sedláček I., Grillová L., Kroupová E., Černohlávková J., Šmajs D.2013. DOI

Sharma M., Kniel K. E., Derevianko A., Ling J., Bhagwat A. A.2007. Sensitivity of PubMed DOI PMC

Shima A., Hinenoya A., Asakura M., Sugimoto N., Tsukamoto T., Ito H., Nagita A., Faruque S. M., Yamasaki S.2012. Molecular characterizations of cytolethal distending toxin produced by PubMed DOI PMC

Slaninová I., Brezinová L., Koubíková L., Slanina J.2009. Dibenzocyclooctadiene lignans overcome drug resistance in lung cancer cells--study of structure-activity relationship. PubMed DOI

Šmajs D., Cejková D., Micenková L., Lima-Bittencourt C. I., Chartone-Souza E., Šmarda J., Nascimento A. M. A.2012. Human PubMed DOI

Šmajs D., Micenková L., Šmarda J., Vrba M., Sevčíková A., Vališová Z., Woznicová V.2010. Bacteriocin synthesis in uropathogenic and commensal PubMed DOI PMC

Šmajs D., Strouhal M., Matejková P., Cejková D., Cursino L., Chartone-Souza E., Šmarda J., Nascimento A. M. A.2008. Complete sequence of low-copy-number plasmid MccC7-H22 of probiotic PubMed DOI

Štaudová B., Micenková L., Bosák J., Hrazdilová K., Slaninková E., Vrba M., Ševčíková A., Kohoutová D., Woznicová V., Bureš J., Šmajs D.2015. Determinants encoding fimbriae type 1 in fecal PubMed DOI PMC

Stock I., Rahman M., Sherwood K. J., Wiedemann B.2005. Natural antimicrobial susceptibility patterns and biochemical identification of PubMed DOI

Švec P., Nováková D., Zácková L., Kukletová M., Sedlácek I.2008. Evaluation of (GTG)5-PCR for rapid identification of PubMed DOI

Švec P., Černohlávková J., Busse H. J., Vojtková H., Pantůček R., Cnockaert M., Mašlaňová I., Králová S., Vandamme P., Sedláček I.2015. Classification of strain CCM 4446 PubMed DOI

Valentiner-Branth P., Steinsland H., Fischer T. K., Perch M., Scheutz F., Dias F., Aaby P., Mølbak K., Sommerfelt H.2003. Cohort study of Guinean children: incidence, pathogenicity, conferred protection, and attributable risk for enteropathogens during the first 2 years of life. PubMed DOI PMC

Walk S. T., Alm E. W., Gordon D. M., Ram J. L., Toranzos G. A., Tiedje J. M., Whittam T. S.2009. Cryptic lineages of the genus PubMed DOI PMC

Wang H., Li Q., Bai X., Xu Y., Zhao A., Sun H., Deng J., Xiao B., Liu X., Sun S., Zhou Y., Wang B., Fan Z., Chen X., Zhang Z., Xu J., Xiong Y.2016. Prevalence of PubMed DOI PMC

Yamamoto D., Hernandes R. T., Liberatore A. M. A., Abe C. M., Souza R. B., Romão F. T., Sperandio V., Koh I. H., Gomes T. A. T.2017. PubMed DOI PMC

Yamamoto S., Terai A., Yuri K., Kurazono H., Takeda Y., Yoshida O.1995. Detection of urovirulence factors in PubMed DOI

Young V. B., Knox K. A., Schauer D. B.2000. Cytolethal distending toxin sequence and activity in the enterohepatic pathogen PubMed DOI PMC