Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem
- MeSH
- dítě MeSH
- DNA bakterií genetika MeSH
- DNA fingerprinting metody MeSH
- fylogeneze MeSH
- lidé MeSH
- polymerázová řetězová reakce metody MeSH
- Streptococcus mutans klasifikace genetika izolace a purifikace MeSH
- streptokokové infekce mikrobiologie MeSH
- techniky typizace bakterií metody MeSH
- zubní plak mikrobiologie MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA bakterií MeSH
Repetitive sequence-based polymerase chain reaction (PCR) fingerprinting using the (GTG)(5) primer was applied for fast screening of bacterial strains isolated from dental plaque of early childhood caries (ECC)-affected children. A group of 29 Gram-positive bacteria was separated into a homogeneous cluster together with Streptococcus mutans reference strains and constituted an aberrant branch after the numerical analysis of (GTG)(5)-PCR fingerprints. Automated ribotyping with EcoRI restriction enzyme (RiboPrinter microbial characterization system) revealed high genetic heterogeneity among the tested group and proved to be a good tool for strain-typing purposes. Further characterization of the studied strains was achieved by extensive phenotyping and whole-cell protein fingerprinting and confirmed all the strains as S. mutans representatives. Obtained results showed rep-PCR fingerprinting with the (GTG)(5) primer to be a fast and reliable method for identification of S. mutans.
Citace poskytuje Crossref.org
(GTG)(5)-PCR fingerprinting of lactobacilli isolated from cervix of healthy women
