Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans
Language English Country Netherlands Media print-electronic
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Child MeSH
- DNA, Bacterial genetics MeSH
- DNA Fingerprinting methods MeSH
- Phylogeny MeSH
- Humans MeSH
- Polymerase Chain Reaction methods MeSH
- Streptococcus mutans classification genetics isolation & purification MeSH
- Streptococcal Infections microbiology MeSH
- Bacterial Typing Techniques methods MeSH
- Dental Plaque microbiology MeSH
- Check Tag
- Child MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Bacterial MeSH
Repetitive sequence-based polymerase chain reaction (PCR) fingerprinting using the (GTG)(5) primer was applied for fast screening of bacterial strains isolated from dental plaque of early childhood caries (ECC)-affected children. A group of 29 Gram-positive bacteria was separated into a homogeneous cluster together with Streptococcus mutans reference strains and constituted an aberrant branch after the numerical analysis of (GTG)(5)-PCR fingerprints. Automated ribotyping with EcoRI restriction enzyme (RiboPrinter microbial characterization system) revealed high genetic heterogeneity among the tested group and proved to be a good tool for strain-typing purposes. Further characterization of the studied strains was achieved by extensive phenotyping and whole-cell protein fingerprinting and confirmed all the strains as S. mutans representatives. Obtained results showed rep-PCR fingerprinting with the (GTG)(5) primer to be a fast and reliable method for identification of S. mutans.
References provided by Crossref.org
(GTG)(5)-PCR fingerprinting of lactobacilli isolated from cervix of healthy women
