Classification of strain CCM 4446T as Rhodococcus degradans sp. nov
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Genes, Bacterial MeSH
- DNA, Bacterial genetics MeSH
- Phospholipids chemistry MeSH
- Phylogeny * MeSH
- Nucleic Acid Hybridization MeSH
- Diaminopimelic Acid chemistry MeSH
- Mycolic Acids chemistry MeSH
- Fatty Acids chemistry MeSH
- Molecular Sequence Data MeSH
- Peptidoglycan chemistry MeSH
- Polyamines chemistry MeSH
- Soil Microbiology * MeSH
- Rhodococcus classification genetics isolation & purification MeSH
- Ribotyping MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Bacterial Typing Techniques MeSH
- Vitamin K 2 chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Switzerland MeSH
- Names of Substances
- DNA, Bacterial MeSH
- Phospholipids MeSH
- Diaminopimelic Acid MeSH
- Mycolic Acids MeSH
- Fatty Acids MeSH
- Peptidoglycan MeSH
- Polyamines MeSH
- RNA, Ribosomal, 16S MeSH
- Vitamin K 2 MeSH
Strain CCM 4446T, with notable biodegradation capabilities, was investigated in this study in order to elucidate its taxonomic position. Chemotaxonomic analyses of quinones, polar lipids, mycolic acids, polyamines and the diamino acid of the cell-wall peptidoglycan corresponded with characteristics of the genus Rhodococcus. Phylogenetic analysis, based on the 16S rRNA gene sequence, assigned strain CCM 4446T to the genus Rhodococcus and placed it in the Rhodococcus erythropolis 16S rRNA gene clade. Further analysis of catA and gyrB gene sequences, automated ribotyping with EcoRI restriction endonuclease, whole-cell protein profiling, DNA-DNA hybridization and extensive biotyping enabled differentiation of strain CCM 4446T from all phylogenetically closely related species, i.e., Rhodococcus baikonurensis, Rhodococcus qingshengii, Rhodococcus erythropolis and Rhodococcus globerulus. The results obtained show that the strain investigated represents a novel species within the genus Rhodococcus, for which the name Rhodococcus degradans sp. nov., is proposed. The type strain is CCM 4446T ( = LMG 28633T).
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