• Publication type
• Retraction Notice MeSH

The aim of this study was to examine the effect of molecular hydrogen (H2) on the healing of alkali-injured cornea. The effects of the solution of H2 in phosphate buffered saline (PBS) or PBS alone topically applied on the alkali-injured rabbit cornea with 0.25 M NaOH were investigated using immunohistochemical and biochemical methods. Central corneal thickness taken as an index of corneal hydration was measured with an ultrasonic pachymeter. Results show that irrigation of the damaged eyes with H2 solution immediately after the injury and then within next five days renewed corneal transparency lost after the injury and reduced corneal hydration increased after the injury to physiological levels within ten days after the injury. In contrast, in injured corneas treated with PBS, the transparency of damaged corneas remained lost and corneal hydration elevated. Later results-on day 20 after the injury-showed that in alkali-injured corneas treated with H2 solution the expression of proinflammatory cytokines, peroxynitrite, detected by nitrotyrosine residues (NT), and malondialdehyde (MDA) expressions were very low or absent compared to PBS treated injured corneas, where NT and MDA expressions were present. In conclusion, H2 solution favorably influenced corneal healing after alkali injury via suppression of oxidative stress.

• MeSH
• Actins metabolism   MeSH
• Cytokines metabolism   MeSH
• Gene Expression drug effects   MeSH
• Sodium Hydroxide toxicity   MeSH
• Immunohistochemistry MeSH
• Interleukin-1beta genetics   metabolism   MeSH
• Keratin-12 metabolism   MeSH
• Keratin-3 metabolism   MeSH
• Rabbits MeSH
• Peroxynitrous Acid metabolism   MeSH
• Malondialdehyde metabolism   MeSH
• Disease Models, Animal MeSH
• Oxidative Stress drug effects   MeSH
• Corneal Injuries etiology   metabolism   pathology   MeSH
• Cornea metabolism   pathology   MeSH
• Vascular Endothelial Growth Factor A genetics   metabolism   MeSH
• Hydrogen pharmacology   MeSH
• Animals MeSH
• Check Tag
• Rabbits MeSH
• Female MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Retracted Publication MeSH
• Names of Substances
• Actins MeSH
• Cytokines MeSH
• Sodium Hydroxide MeSH
• Interleukin-1beta MeSH
• Keratin-12 MeSH
• Keratin-3 MeSH
• Peroxynitrous Acid MeSH
• Malondialdehyde MeSH
• Vascular Endothelial Growth Factor A MeSH
• Hydrogen MeSH