The Favorable Effect of Mesenchymal Stem Cell Treatment on the Antioxidant Protective Mechanism in the Corneal Epithelium and Renewal of Corneal Optical Properties Changed after Alkali Burns
Status odvoláno Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem, publikace stažené z tisku
PubMed
27057279
PubMed Central
PMC4736412
DOI
10.1155/2016/5843809
Knihovny.cz E-zdroje
- MeSH
- alkálie MeSH
- antioxidancia terapeutické užití MeSH
- buněčná diferenciace účinky léků MeSH
- chemické popálení enzymologie genetika patologie terapie MeSH
- imunohistochemie MeSH
- králíci MeSH
- limbus corneae cytologie MeSH
- matrixová metaloproteinasa 9 metabolismus MeSH
- mezenchymální kmenové buňky cytologie účinky léků MeSH
- ochranné látky farmakologie terapeutické užití MeSH
- pachymetrie rohovky MeSH
- regulace genové exprese účinky léků MeSH
- rohovkový epitel patologie MeSH
- superoxiddismutasa metabolismus MeSH
- synthasa oxidu dusnatého, typ II metabolismus MeSH
- transformující růstový faktor beta genetika metabolismus MeSH
- transplantace mezenchymálních kmenových buněk * MeSH
- tukové buňky cytologie účinky léků MeSH
- vaskulární endoteliální růstový faktor A metabolismus MeSH
- zákal rohovky komplikace terapie MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- publikace stažené z tisku MeSH
- Názvy látek
- alkálie MeSH
- antioxidancia MeSH
- matrixová metaloproteinasa 9 MeSH
- ochranné látky MeSH
- superoxiddismutasa MeSH
- synthasa oxidu dusnatého, typ II MeSH
- transformující růstový faktor beta MeSH
- vaskulární endoteliální růstový faktor A MeSH
The aim of this study was to examine whether mesenchymal stem cells (MSCs) and/or corneal limbal epithelial stem cells (LSCs) influence restoration of an antioxidant protective mechanism in the corneal epithelium and renewal of corneal optical properties changed after alkali burns. The injured rabbit corneas (with 0.25 N NaOH) were untreated or treated with nanofiber scaffolds free of stem cells, with nanofiber scaffolds seeded with bone marrow MSCs (BM-MSCs), with adipose tissue MSCs (Ad-MSCs), or with LSCs. On day 15 following the injury, after BM-MSCs or LSCs nanofiber treatment (less after Ad-MSCs treatment) the expression of antioxidant enzymes was restored in the regenerated corneal epithelium and the expressions of matrix metalloproteinase 9 (MMP9), inducible nitric oxide synthase (iNOS), α-smooth muscle actin (α-SMA), transforming growth factor-β1 (TGF-β1), and vascular endothelial factor (VEGF) were low. The central corneal thickness (taken as an index of corneal hydration) increased after the injury and returned to levels before the injury. In injured untreated corneas the epithelium was absent and numerous cells revealed the expressions of iNOS, MMP9, α-SMA, TGF-β1, and VEGF. In conclusion, stem cell treatment accelerated regeneration of the corneal epithelium, restored the antioxidant protective mechanism, and renewed corneal optical properties.
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Molecular Hydrogen Effectively Heals Alkali-Injured Cornea via Suppression of Oxidative Stress