A new E. coli-S. cerevisiae shuttle plasmid cloning vector (pPW263) with a positive type of selection, was constructed. The selection system, based on the regulatory region of lambda phage controlling the expression of tetracycline resistance, was derived from the cloning vector pUN121 (Nilsson et al. 1983). There are three cloning sites in the cI gene, EcoRI, HindIII and BglII, and, in addition, two unique sites in the neighborhood, BamHI and SalI. The size of the vector is 7.8 kb. The maintenance of the vector and the selection in yeast was ensured by the replication region of the 2 mu plasmid and by the URA3 marker gene, respectively.

• MeSH
• Escherichia coli genetics   MeSH
• Genetic Vectors MeSH
• Cloning, Molecular methods   MeSH
• Molecular Sequence Data MeSH
• Mutagenesis, Site-Directed MeSH
• Plasmids * MeSH
• Restriction Mapping MeSH
• Saccharomyces cerevisiae genetics   MeSH
• Amino Acid Sequence MeSH
• Base Sequence MeSH
• Publication type
• Journal Article MeSH

The nucleotide sequence of an 8.2-kb DNA fragment from the 5' proximal part of the chicken myb proto-oncogene spanning 1761 nucleotides upstream and 6436 nucleotides downstream from a presumed c-myb initiation codon was determined. A 3.3-kb G + C-rich region found in this sequence had also other features characterizing CpG islands, i.e. no CpG underrepresentation and lack of CpG methylation. In haematopoietic tissues c-myb mRNA synthesis starts in two major regions of the CpG island, namely 98 to 108 and 143 to 145 nucleotides upstream from the c-myb initiation codon. These two regions are in or close to the 124-bp evolutionarily conserved element located in the middle part of the CpG island. No alternative splicing of the 5' end of c-myb mRNA suggested earlier (1,2) was observed. The c-myb promoter contains neither TATA nor CAAT box-like structures at the usual positions. Instead, numerous potential Sp1 factor binding sites were found both upstream and downstream from the transcription initiation sites. Moreover, consensus v-myb protein DNA-binding sites were revealed in the promoter region and in sequences downstream from it.

• MeSH
• Dinucleoside Phosphates analysis   MeSH
• Single-Strand Specific DNA and RNA Endonucleases MeSH
• Endonucleases MeSH
• Erythrocytes analysis   MeSH
• Transcription, Genetic * MeSH
• Cloning, Molecular MeSH
• Chickens MeSH
• Molecular Sequence Data MeSH
• Mice MeSH
• Organ Specificity MeSH
• Promoter Regions, Genetic MeSH
• Proto-Oncogene Proteins c-myb MeSH
• Proto-Oncogene Proteins genetics   MeSH
• Proto-Oncogenes * MeSH
• Restriction Mapping MeSH
• RNA Caps genetics   MeSH
• Base Sequence MeSH
• Sequence Homology, Nucleic Acid MeSH
• Thymus Gland analysis   MeSH
• Animals MeSH
• Check Tag
• Male MeSH
• Mice MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Comparative Study MeSH
• Names of Substances
• cytidylyl-3'-5'-guanosine MeSH Browser
• Dinucleoside Phosphates MeSH
• Single-Strand Specific DNA and RNA Endonucleases MeSH
• Endonucleases MeSH
• Proto-Oncogene Proteins c-myb MeSH
• Proto-Oncogene Proteins MeSH
• RNA Caps MeSH