This study explores the innovative approach in the development of freeze-dried hydrogel films, leveraging the unique properties of gum Karaya (GK), poly-(vinyl alcohol) (PVA), poly-(ethylene glycol) (PEG), and glycerol with a coating of octenidine dihydrochloride (OCT). These innovative hydrogel films exhibit at a certain glycerol concentration a sandwich-like structure, achieved through a tailored freeze-drying process, which enhances transparency and mechanical stability. OCT provides superior antibacterial performance, effectively combating multidrug-resistant bacteria with a controlled and gradual release mechanism, surpassing conventional OCT solutions that require frequent reapplication for infected wound treatment without the creation of bacterial resistance. Advanced environmental scanning electron microscopy (A-ESEM) reveals the complex microstructure of the hydrogel, highlighting the dense surface layer and interconnected porous bulk. Variations in glycerol concentrations proved to significantly impact hydrogels' properties. Increasing the glycerol concentration decreases the pore size (around 4.5 μm) while enhancing the polymer network density and flexibility. However, low concentration increases the pore size (7.8-15.6 μm), impacting enhanced swelling behavior and hydrolytic stability. OCT's rapid antibacterial action, releasing over 30% within the first hour and maintaining prolonged activity for up to 2 weeks, emphasizes the material's potential for diverse applications. Hydrogels' remarkable transparency, porosity, structural stability, and antibacterial efficacy against both Gram-positive and Gram-negative strains suggest promising uses as transparent dressings, biomedical devices, and infection-resistant surfaces.

• Publikační typ
• časopisecké články MeSH

The present article describes the state of the art in the rapidly developing field of bone tissue engineering, where many disciplines, such as material science, mechanical engineering, clinical medicine and genetics, are interconnected. The main objective is to restore and improve the function of bone tissue by scaffolds, providing a suitable environment for tissue regeneration and repair. Strategies and materials used in oral regenerative therapies correspond to techniques generally used in bone tissue engineering. Researchers are focusing on developing and improving new materials to imitate the native biological neighborhood as authentically as possible. The most promising is a combination of cells and matrices (scaffolds) that can be fabricated from different kinds of materials. This review summarizes currently available materials and manufacturing technologies of scaffolds for bone-tissue regeneration.

• Klíčová slova
• bone tissue engineering, hydrogels, regenerative medicine, scaffolds, stem cells,
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH