The total arsenic mass fraction as well as the arsenic speciation were studied in four different mushroom species with inductively coupled plasma mass spectrometry and high-performance liquid chromatography coupled to inductively coupled plasma mass spectrometry, respectively. Arsenic mass fractions detected in the mushrooms were covering a range from 0.3 to 22 mg As kg-1 dry mass. For the arsenic speciation, species like arsenobetaine, inorganic arsenic, or dimethylarsinic acid were found, which are commonly detected in mushrooms, but it was also proven that the recently discovered novel compound homoarsenocholine is present in Amanita muscaria and Ramaria sanguinea. Moreover, a previously unidentified arsenic species was isolated from Ramaria sanguinea and identified as trimethylarsonioacetamide, or in short: arsenobetaine amide. This new arsenical was synthesized and verified by spiking experiments to be present in all investigated mushroom samples. Arsenobetaine amide could be an important intermediate to further elucidate the biotransformation pathways of arsenic in the environment.

• Klíčová slova
• Arsenic speciation, HPLC-ICPMS, HR ESI-MS, Mushrooms, Ramaria sanguinea, Trimethylarsonioacetamide,
• MeSH
• arsen * analýza   MeSH
• arsenikové přípravky * analýza   MeSH
• Basidiomycota * MeSH
• hmotnostní spektrometrie metody   MeSH
• vysokoúčinná kapalinová chromatografie metody   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• arsen * MeSH
• arsenikové přípravky * MeSH
• arsenobetaine MeSH Prohlížeč

The arsenic speciation was determined in macrofungi of the Ramaria genus with HPLC coupled to inductively coupled plasma mass spectrometry. Besides arsenic species that are already known for macrofungi, like arsenobetaine or arsenocholine, two compounds that were only known from marine samples so far (trimethylarsoniopropanate and dimethylarsinoylacetate) were found for the first time in a terrestrial sample. An unknown arsenical was isolated and identified as homoarsenocholine. This could be a key intermediate for further elucidation of the biotransformation mechanisms of arsenic.

• Klíčová slova
• (3-hydroxypropyl) trimethylarsonium ion, Arsenic speciation, Fungi, Homoarsenocholine, ICPMS, Ramaria,
• MeSH
• acetáty chemie   izolace a purifikace   MeSH
• arsenikové přípravky chemie   izolace a purifikace   MeSH
• Basidiomycota chemie   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• acetáty MeSH
• arsenikové přípravky MeSH
• dimethylarsinoylacetic acid MeSH Prohlížeč