The total arsenic mass fraction as well as the arsenic speciation were studied in four different mushroom species with inductively coupled plasma mass spectrometry and high-performance liquid chromatography coupled to inductively coupled plasma mass spectrometry, respectively. Arsenic mass fractions detected in the mushrooms were covering a range from 0.3 to 22 mg As kg-1 dry mass. For the arsenic speciation, species like arsenobetaine, inorganic arsenic, or dimethylarsinic acid were found, which are commonly detected in mushrooms, but it was also proven that the recently discovered novel compound homoarsenocholine is present in Amanita muscaria and Ramaria sanguinea. Moreover, a previously unidentified arsenic species was isolated from Ramaria sanguinea and identified as trimethylarsonioacetamide, or in short: arsenobetaine amide. This new arsenical was synthesized and verified by spiking experiments to be present in all investigated mushroom samples. Arsenobetaine amide could be an important intermediate to further elucidate the biotransformation pathways of arsenic in the environment.

Faculty of Chemistry Institute of Analytical Chemistry University of Vienna Waehringer Strasse 38 1090 Vienna Austria

Institute of Chemistry Analytical Chemistry University of Graz Universitaetsplatz 1 8010 Graz Austria

Institute of Chemistry Inorganic Chemistry University of Graz Universitaetsplatz 1 8010 Graz Austria

Institute of Geology of the Czech Academy of Sciences Rozvojová 269 16500 Prague 6 Czech Republic

Nuclear Physics Institute of the Czech Academy of Sciences Hlavní 130 25068 Husinec Řež Czech Republic

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