Neuroblastoma represents 8-10 % of all malignant tumors in childhood and is responsible for 15 % of cancer deaths in the pediatric population. Aggressive neuroblastomas are often resistant to chemotherapy. Canonically, neuroblastomas can be classified according to the MYCN (N-myc proto-oncogene protein) gene amplification, a common marker of tumor aggressiveness and poor prognosis. It has been found that certain compounds with chelating properties may show anticancer activity, but there is little evidence for the effect of chelators on neuroblastoma. The effect of new chelators characterized by the same functional group, designated as HLZ (1-hydrazino phthalazine), on proliferation (WST-1 and methylene blue assay), cell cycle (flow cytometry), apoptosis (proliferation assay after use of specific pharmacological inhibitors and western blot analysis) and ROS production (fluorometric assay based on dichlorofluorescein diacetate metabolism) was studied in three neuroblastoma cell lines with different levels of MYCN amplification. The molecules were effective only on MYCN-non-amplified cells in which they arrested the cell cycle in the G0/G1 phase. We investigated the mechanism of action and identified the activation of cell signaling that involves protein kinase C.

• MeSH
• apoptóza MeSH
• chelátory farmakologie   terapeutické užití   MeSH
• dítě MeSH
• jaderné proteiny genetika   MeSH
• lidé MeSH
• nádorové buněčné linie MeSH
• neuroblastom * farmakoterapie   MeSH
• onkogenní proteiny * genetika   metabolismus   farmakologie   MeSH
• proliferace buněk MeSH
• protoonkogen n-myc genetika   metabolismus   terapeutické užití   MeSH
• regulace genové exprese u nádorů MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• chelátory MeSH
• jaderné proteiny MeSH
• onkogenní proteiny * MeSH
• protoonkogen n-myc MeSH