Knowledge of mass transport parameters, diffusion, and viscosity of hyaluronic acid (HA) in the presence of cyclodextrins is of considerable importance for areas such as food packaging and drug delivery, among others. Despite a number of studies investigating the functionalization of HA or the corresponding sodium salt by cyclodextrins, only a few studies have reported the effect of cyclodextrins on the mass transport of HA in the presence of these oligosaccharides. Here, we report the tracer binary and ternary interdiffusion coefficients of sodium hyaluronate (NaHy) in water and aqueous β-cyclodextrin solutions. The diffusion behavior of sodium hyaluronate was dependent on the reduced viscosity of NaHy, which, in turn, presented a concave dependence on concentration, with a minimum at approximately 2.5 g dm-3. The significant decrease in the limiting diffusion coefficient of NaHy (at most 45%) at NaHy concentrations below 1 g dm-3 in the presence of β-cyclodextrin, taking water as the reference, allowed us to conclude that NaHy strongly interacted with the cyclodextrin.

• Klíčová slova
• diffusion, diffusion coefficients, salting-in, sodium hyaluronate, transport properties, viscosity, β-cyclodextrin,
• MeSH
• beta-cyklodextriny * MeSH
• cyklodextriny * MeSH
• difuze MeSH
• kyselina hyaluronová MeSH
• voda MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• beta-cyklodextriny * MeSH
• cyklodextriny * MeSH
• kyselina hyaluronová MeSH
• voda MeSH

Tracer diffusion coefficients obtained from the Taylor dispersion technique at 25.0 °C were measured to study the influence of sodium, ammonium and magnesium salts at 0.01 and 0.1 mol dm-3 on the transport behavior of sodium hyaluronate (NaHy, 0.1%). The selection of these salts was based on their position in Hofmeister series, which describe the specific influence of different ions (cations and anions) on some physicochemical properties of a system that can be interpreted as a salting-in or salting-out effect. In our case, in general, an increase in the ionic strength (i.e., concentrations at 0.01 mol dm-3) led to a significant decrease in the limiting diffusion coefficient of the NaHy 0.1%, indicating, in those circumstances, the presence of salting-in effects. However, the opposite effect (salting-out) was verified with the increase in concentration of some salts, mainly for NH4SCN at 0.1 mol dm-3. In this particular salt, the cation is weakly hydrated and, consequently, its presence does not favor interactions between NaHy and water molecules, promoting, in those circumstances, less resistance to the movement of NaHy and thus to the increase of its diffusion (19%). These data, complemented by viscosity measurements, permit us to have a better understanding about the effect of these salts on the transport behaviour of NaHy.

• Klíčová slova
• Hofmeister series, sodium hyaluronate, transport properties, viscosity,
• MeSH
• anionty chemie   MeSH
• biologický transport MeSH
• chlorid lithný chemie   MeSH
• chlorid sodný chemie   MeSH
• difuze MeSH
• kationty chemie   MeSH
• kyselina hyaluronová chemie   MeSH
• osmolární koncentrace MeSH
• roztoky MeSH
• síran amonný chemie   MeSH
• síran hořečnatý chemie   MeSH
• sírany chemie   MeSH
• soli chemie   MeSH
• teplota MeSH
• thiokyanatany chemie   MeSH
• viskozita MeSH
• voda chemie   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• anionty MeSH
• chlorid lithný MeSH
• chlorid sodný MeSH
• kationty MeSH
• kyselina hyaluronová MeSH
• roztoky MeSH
• síran amonný MeSH
• síran hořečnatý MeSH
• sírany MeSH
• sodium sulfate MeSH Prohlížeč
• sodium thiocyanate MeSH Prohlížeč
• soli MeSH
• thiocyanic acid MeSH Prohlížeč
• thiokyanatany MeSH
• voda MeSH

In the current study, we present methods of sodium hyaluronate, also denoted as hyaluronan (HA), nanofiber fabrication using a direct-current (DC) electric field. HA was spun in combination with poly(vinyl alcohol) (PVA) and polyethylene oxide (PEO) and as a pure polymer. Nonaggressive solvents were used due to the possible use of the fibers in life sciences. The influences of polymer concentration, average molecular weight (Mw), viscosity, and solution surface tension were analyzed. HA and PVA were fluorescent-labeled in order to examine the electrospun structures using fluorescence confocal microscopy. In this study, two intermediate solvent mixtures that facilitate HA electrospinning were found. In the case of polymer co-electrospinning, the effect of the surfactant content on the HA/PVA electrospinning process, and the effect of HA Mw on HA/PEO nanofiber morphology, were examined, respectively.

• Klíčová slova
• electrospinning, fluorescence confocal microscopy, hyaluronan, intermediate solvent, nanofibers, poly(vinyl alcohol), polyethylene oxide,
• Publikační typ
• časopisecké články MeSH