Oligosaccharides produced by submerged cultures of C. africana and C. sorghi were isolated by semipreparative HPLC. Structure of 6-O-beta-D-fructofuranosyl-D-glucopyranose (blastose), 1,6-bis-O-(beta-D-fructofuranosyl)-alpha-D-glucopyranoside (neokestose) and two sugar alcohols, 1-O-beta-D-fructofuranosyl-D-mannitol (fructosylmannitol) and 1,6-bis-O-(beta-D-fructofuranosyl)-D-mannitol (bisfructosylmannitol) was determined by NMR spectrometry. MALDI TOF MS analysis revealed molecular ions [M+Na]+ that indicate the presence of other tetra- and pentasaccharides (m/z = 689.4 and 851.5, respectively) and corresponding sugar alcohol (m/z = 691.4). Rapid conversion of sucrose into series of oligosaccharides and corresponding sugar alcohols was observed in all tested strains.

• MeSH
• Claviceps růst a vývoj   izolace a purifikace   metabolismus   patogenita   MeSH
• cukerné alkoholy chemie   metabolismus   MeSH
• fermentace MeSH
• molekulární struktura MeSH
• nemoci rostlin mikrobiologie   MeSH
• nukleární magnetická rezonance biomolekulární MeSH
• oligosacharidy biosyntéza   chemie   izolace a purifikace   MeSH
• Sorghum mikrobiologie   MeSH
• spektrometrie hmotnostní - ionizace laserem za účasti matrice MeSH
• vysokoúčinná kapalinová chromatografie MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• cukerné alkoholy MeSH
• oligosacharidy MeSH

The response of the cenosis composition of soil saprotrophic microfungi able to utilize the fulvic fraction of soil organic matter to increased concentration of atmospheric carbon dioxide, plant species cover quality and different levels of nitrogen fertilization was determined under field conditions in a free-air carbon dioxide enrichment experiment. Twenty-nine species of microfungi were isolated from the tested soil. The effects of CO2 enrichment and plant species cover were not significant. Nitrogen fertilization was identified as the only significant factor inducing changes in the abundance of soil microorganisms. This was reflected in a relatively low value of quantitative Sørensen similarity index on comparing fertilized and unfertilized treatments and in 2-way ANOVA of total CFU counts. Some differences were observed in species diversity between the two variants of all treatments. No association between microfungi and the factors under study was found by using the Monte Carlo Permutation test in redundancy analysis.

• MeSH
• benzopyrany metabolismus   MeSH
• biodegradace MeSH
• DNA fungální genetika   MeSH
• druhová specificita MeSH
• dusík metabolismus   MeSH
• ekosystém MeSH
• houby klasifikace   genetika   izolace a purifikace   metabolismus   MeSH
• oxid uhličitý metabolismus   MeSH
• počet mikrobiálních kolonií MeSH
• půda analýza   MeSH
• půdní mikrobiologie * MeSH
• rostliny metabolismus   mikrobiologie   MeSH
• sekvence nukleotidů MeSH
• životní prostředí MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Švýcarsko MeSH
• Názvy látek
• benzopyrany MeSH
• DNA fungální MeSH
• dusík MeSH
• fulvic acid MeSH Prohlížeč
• oxid uhličitý MeSH
• půda MeSH