Cytoplasmic male sterility (CMS), encoded by the interacting mitochondrial and nuclear genes, causes pollen abortion or non-viability. CMS is widely used in agriculture and extensively studied in crops. Much less is known about CMS in wild species. We performed a comparative transcriptomic analysis of male sterile and fertile individuals of Silene vulgaris, a model plant for the study of gynodioecy, to reveal the genes responsible for pollen abortion in this species. We used RNA-seq datasets previously employed for the analysis of mitochondrial and plastid transcriptomes of female and hermaphrodite flower buds, making it possible to compare the transcriptomes derived from three genomes in the same RNA specimen. We assembled de novo transcriptomes for two haplotypes of S. vulgaris and identified differentially expressed genes between the females and hermaphrodites, associated with stress response or pollen development. The gene for alternative oxidase was downregulated in females. The genetic pathways controlling CMS in S. vulgaris are similar to those in crops. The high number of the differentially expressed nuclear genes contrasts with the uniformity of organellar transcriptomes across genders, which suggests these pathways are evolutionarily conserved and that selective mechanisms may shield organellar transcription against changes in the cytoplasmic transcriptome.

• Klíčová slova
• Silene vulgaris, cytoplasmic male sterility, differential gene expression,
• MeSH
• anotace sekvence MeSH
• buněčné jádro genetika   MeSH
• down regulace genetika   MeSH
• genová ontologie MeSH
• haplotypy genetika   MeSH
• messenger RNA genetika   metabolismus   MeSH
• mitochondriální proteiny genetika   metabolismus   MeSH
• neplodnost rostlin genetika   MeSH
• oxidační stres genetika   MeSH
• oxidoreduktasy genetika   metabolismus   MeSH
• pyl genetika   MeSH
• regulace genové exprese u rostlin * MeSH
• rostlinné proteiny genetika   metabolismus   MeSH
• Silene genetika   fyziologie   MeSH
• stanovení celkové genové exprese * MeSH
• transkriptom genetika   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• alternative oxidase MeSH Prohlížeč
• messenger RNA MeSH
• mitochondriální proteiny MeSH
• oxidoreduktasy MeSH
• rostlinné proteiny MeSH