Pythium oligandrum, a soil-born oomycete, is an effective biological control agent exhibiting antagonistic and parasitic activity against pathogenic fungi. This study is the first attempt to characterize its surface properties and to apply models of physicochemical interactions (thermodynamic, DLVO and XDLVO) to quantify its adhesion properties to a model material, represented by magnetic beads (MB). The predictions of interaction models were based on experimental data (contact angles, zeta potentials, size). Adhesion intensities (AI) were determined experimentally taking advantage of MB with different surface properties. The role of weak physicochemical interactions was estimated by comparing experimental AI with model predictions. The results revealed that the surface properties of the three Pythium spp. studied were very similar and fell within the range for hydrophilic microorganisms (ΔGTOT > 0) with a predominantly negative surface charge. The most reliable description of AI was obtained using the DLVO model, including Lifshitz-van der Waals and electrostatic interactions. The highest AI between Pythium spp. and all three MB was observed at pH 3, which was supported by the DLVO prediction. The greater agreement between the sphere-sphere geometric version of the DLVO model and experiment suggests that the surface protrusions of the oospores increase the efficiency of adhesion. The surface properties of the pathogenic fungi, characterized in this work, fell within the range defined by MB and therefore it can be expected that their physicochemical interactions with Pythium spp. will also be favourable.

• Keywords
• (X)DLVO model, Adhesion, Model materials, Pythium species, Surface interaction, Thermodynamic model,
• Publication type
• Journal Article MeSH

INTRODUCTION: Biological control of root diseases of lucerne (Medicago sativa L.) has potential benefits for stand performance but this remains unsupported by evidence from practical field studies. METHODS: In field experiments at three sites our objectives were to determine the effect of Pythium oligandrum, as spring, autumn and intensive regime treatments on (i) lucerne plant density and root traits development, and (ii) forage yield and forage traits. Lucerne stands were managed under two or three treatments: non-treated control and P. oligandrum applied at two intensities of application under four-cut utilization. RESULTS AND DISCUSSION: Under relatively dry conditions (annual mean 10°C and <500 mm precipitation) lucerne dry matter yield was significantly reduced by 6%, which could be related to mechanisms of inappropriate stimulation and disturbance of the balance between auxins and ethylene. Under annual precipitation of >500 mm, positive impacts on stand height or fine root mass were observed for the autumn and intensive treatments where positive root response was visible only in alluvial soil. However, these changes did not result in higher yield and probably more applications per year will be needed for significant forage yield improvement. This study highlights the limits of field-scale biological control in which the potential of P. oligandrum for lucerne productivity improvement was realised only under a humid environment or deep alluvial soils, where higher root disease infestation may also be expected.

• Keywords
• Medicago sativa, Polyversum, alfalfa, dry conditions, plant growth promotion,
• Publication type
• Journal Article MeSH

Pythium oligandrum, strain M1, is a soil oomycete successfully used as a biological control agent (BCA), protecting plants against fungal, yeast, and oomycete pathogens through mycoparasitism and elicitor-dependent plant priming. The not yet described Pythium strains, X42 and 00X48, have shown potential as BCAs given the high activity of their secreted proteases, endoglycosidases, and tryptamine. Here, Solanum lycopersicum L. cv. Micro-Tom seeds were coated with Pythium strains, and seedlings were exposed to fungal pathogens, either Alternaria brassicicola or Verticillium albo-atrum. The effects of both infection and seed-coating on plant metabolism were assessed by determining the activity and isoforms of antioxidant enzymes and endoglycosidases and the content of tryptamine, amino acids, and heat shock proteins. Dual culture competition testing and microscopy analysis confirmed mycoparasitism in all three Pythium strains. In turn, seed treatment significantly increased the total free amino acid content, changing their abundance in both non-infected and infected plants. In response to pathogens, plant Hsp70 and Hsp90 isoform levels also varied among Pythium strains, most likely as a strategy for priming the plant against infection. Overall, our results show in vitro mycoparasitism between Pythium strains and fungal pathogens and in planta involvement of heat shock proteins in priming.

• Keywords
• antioxidants, capillary electrophoresis, fungal diseases, plant protection, seed-coating,
• Publication type
• Journal Article MeSH