The presented work brings new knowledge in the field of spinning electrodes for AC‑electrospinning technology, which is used for producing nanofibrous structures using a solution of polyvinyl butyral. It presents new types of spinning weir‑electrodes and describes research on the influence of electrode design parameters on the stability of the spinning process and the productivity of nanofiber production. The multistage spinning electrode is presented in the ratio of stages one to five. Research is also focused on the effect of the parameters of the electric signal used as a source for the spinning electrode on spinning stability and productivity. Observed parameters were voltage level, frequency and shape such as sine wave, rectangle wave and modified sine wave. An analysis of the influence of the spinning conditions on the resulting nanofibrous structure was also performed by analyzing results gained by SEM; the defects were investigated mainly. The results of the research presented in the thesis open up new possibilities for follow-up research in the field of AC-electrospinning.

• Klíčová slova
• AC electrospinning, Electric field, Electrospinning, Nanofibers, Spinning electrode,
• Publikační typ
• časopisecké články MeSH

The ever-increasing demands of modern medicine drive the development of novel drug delivery materials. In particular, nanofibers are promising for such materials due to their favorable properties. However, most development is still carried out through laboratory techniques that do not allow extensive and reproducible characterization of materials, which slows medical research. In this work, we focus on the large-scale fabrication and testing of specific antibacterial nanofibrous materials to prevent the postoperative complications associated with the occurrence of bacterial infection. Poly-ε-caprolactone with gentamicin sulfate (antibiotic) in different concentrations was electrospun via a needleless device. The amount of antibiotics was proven by elemental analysis, UV spectrophotometry, and HPLC. The cytocompatibility of the materials was verified in vitro according to ISO 10993-5. The cell adhesion and proliferation were assessed after 2, 7, 14, and 21 days using the CCK-8 metabolic assay, fluorescence, and scanning electron microscopy. The tested nanofiber materials supported cell growth. Antibacterial tests were performed to confirm the release of gentamicin sulfate, and its antibacterial properties were proven toward Staphylococcus gallinarum and Escherichia coli bacteria. The effect of ethylene oxide sterilization was also studied. The sterilized nanofibrous layers are cytocompatible while antibacterial and therefore suitable for medical applications.

• Publikační typ
• časopisecké články MeSH

Undesirable postoperative tissue adhesions remain among the most common complications after surgery. Apart from pharmacological antiadhesive agents, various physical barriers have been developed in order to prevent postoperative tissue adhesions. Nevertheless, many introduced materials suffer from shortcomings during in vivo application. Thus, there is an increasing need to develop a novel barrier material. However, various challenging criteria have to be met, so this issue pushes the research in materials to its current limits. Nanofibers play a major role in breaking the wall of this issue. Due to their properties, such as a large surface area for functionalization, tunable degradation rate, or the possibility of layering individual nanofibrous materials, it is feasible to create an antiadhesive surface while maintaining biocompatibility. There are many ways to produce nanofibrous material; electrospinning is the most used and versatile technique. This review reveals the different approaches and puts them into context.

• Publikační typ
• časopisecké články MeSH
• přehledy MeSH