Systems of antigen delivery into antigen-presenting cells represent an important novel strategy in chicken vaccine development. In this study, we verified the ability of Rous sarcoma virus (RSV) antigens fused with streptavidin to be targeted by specific biotinylated monoclonal antibody (anti-CD205) into dendritic cells and induce virus-specific protective immunity. The method was tested in four congenic lines of chickens that are either resistant or susceptible to the progressive growth of RSV-induced tumors. Our analyses confirmed that the biot-anti-CD205-SA-FITC complex was internalized by chicken splenocytes. In the cytokine expression profile, several significant differences were evident between RSV-challenged progressor and regressor chicken lines. A significant up-regulation of IL-2, IL-12, IL-15, and IL-18 expression was detected in immunized chickens of both regressor and progressor groups. Of these cytokines, IL-2 and IL-12 were most up-regulated 14 days post-challenge (dpc), while IL-15 and IL-18 were most up-regulated at 28 dpc. On the contrary, IL-10 expression was significantly down-regulated in all immunized groups of progressor chickens at 14 dpc. We detected significant up-regulation of IL-17 in the group of immunized progressors. LITAF down-regulation with iNOS up-regulation was especially observed in the progressor group of immunized chickens that developed large tumors. Based on the increased expression of cytokines specific for activated dendritic cells, we conclude that our system is able to induce partial stimulation of specific cell types involved in cell-mediated immunity.

• MeSH
• Antigens, Viral immunology   MeSH
• Immunity, Cellular immunology   MeSH
• Antigens, CD immunology   MeSH
• Cytokines physiology   MeSH
• Dendritic Cells immunology   virology   MeSH
• Chickens immunology   virology   MeSH
• Lectins, C-Type immunology   MeSH
• Antibodies, Bispecific immunology   MeSH
• Sarcoma, Avian immunology   prevention & control   MeSH
• Receptors, Cell Surface immunology   MeSH
• Minor Histocompatibility Antigens immunology   MeSH
• Viral Vaccines immunology   MeSH
• Rous sarcoma virus immunology   MeSH
• Animals, Congenic immunology   virology   MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Antigens, Viral MeSH
• Antigens, CD MeSH
• Cytokines MeSH
• DEC-205 receptor MeSH Browser
• Lectins, C-Type MeSH
• Antibodies, Bispecific MeSH
• Receptors, Cell Surface MeSH
• Minor Histocompatibility Antigens MeSH
• Viral Vaccines MeSH

• MeSH
• Crossing Over, Genetic * MeSH
• Genotype * MeSH
• Haplotypes MeSH
• Major Histocompatibility Complex genetics   MeSH
• Chickens MeSH
• Polymorphism, Restriction Fragment Length MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Journal Article MeSH

We report that the cloned DNA harboring the long terminal repeat (LTR), v-src, LTR proviral structure is tumorigenic in chickens of the Prague congenic lines. The growth rate of these tumors is by far the highest in the recombinant CC.R1 line, the B haplotype of which is composed of the B-F/L4 and B-G12 subregions originating from different naturally occurring haplotypes. Some of the tumors induced by the LTR, v-src, LTR DNA are repeatedly transplantable in syngeneic chickens, maintain unaltered provirus, and express v-src mRNA. Differences in the response to challenge with Rous sarcoma virus (RSV) and LTR, v-src, LTR DNA on a given experimental model are compared and possible involvement of an interaction between B-F/L and B-G region genes is considered. Regression of the LTR, v-src, LTR DNA-induced tumors did not prevent the formation and growth of tumors induced subsequently by RSV.

• MeSH
• Cell Division genetics   MeSH
• DNA, Neoplasm physiology   MeSH
• Genes, src physiology   MeSH
• Cloning, Molecular MeSH
• Chickens MeSH
• Neoplasms genetics   microbiology   MeSH
• Blotting, Northern MeSH
• Polymorphism, Restriction Fragment Length MeSH
• Proviruses MeSH
• Repetitive Sequences, Nucleic Acid physiology   MeSH
• Blotting, Southern MeSH
• Genetic Complementation Test MeSH
• Avian Sarcoma Viruses MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• DNA, Neoplasm MeSH