Systems of antigen delivery into antigen-presenting cells represent an important novel strategy in chicken vaccine development. In this study, we verified the ability of Rous sarcoma virus (RSV) antigens fused with streptavidin to be targeted by specific biotinylated monoclonal antibody (anti-CD205) into dendritic cells and induce virus-specific protective immunity. The method was tested in four congenic lines of chickens that are either resistant or susceptible to the progressive growth of RSV-induced tumors. Our analyses confirmed that the biot-anti-CD205-SA-FITC complex was internalized by chicken splenocytes. In the cytokine expression profile, several significant differences were evident between RSV-challenged progressor and regressor chicken lines. A significant up-regulation of IL-2, IL-12, IL-15, and IL-18 expression was detected in immunized chickens of both regressor and progressor groups. Of these cytokines, IL-2 and IL-12 were most up-regulated 14 days post-challenge (dpc), while IL-15 and IL-18 were most up-regulated at 28 dpc. On the contrary, IL-10 expression was significantly down-regulated in all immunized groups of progressor chickens at 14 dpc. We detected significant up-regulation of IL-17 in the group of immunized progressors. LITAF down-regulation with iNOS up-regulation was especially observed in the progressor group of immunized chickens that developed large tumors. Based on the increased expression of cytokines specific for activated dendritic cells, we conclude that our system is able to induce partial stimulation of specific cell types involved in cell-mediated immunity.

• MeSH
• antigeny virové imunologie   MeSH
• buněčná imunita imunologie   MeSH
• CD antigeny imunologie   MeSH
• cytokiny fyziologie   MeSH
• dendritické buňky imunologie   virologie   MeSH
• kur domácí imunologie   virologie   MeSH
• lektiny typu C imunologie   MeSH
• protilátky bispecifické imunologie   MeSH
• ptačí sarkom imunologie   prevence a kontrola   MeSH
• receptory buněčného povrchu imunologie   MeSH
• vedlejší histokompatibilní antigeny imunologie   MeSH
• virové vakcíny imunologie   MeSH
• virus Rousova sarkomu imunologie   MeSH
• zvířata kongenní imunologie   virologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny virové MeSH
• CD antigeny MeSH
• cytokiny MeSH
• DEC-205 receptor MeSH Prohlížeč
• lektiny typu C MeSH
• protilátky bispecifické MeSH
• receptory buněčného povrchu MeSH
• vedlejší histokompatibilní antigeny MeSH
• virové vakcíny MeSH

• MeSH
• crossing over (genetika) * MeSH
• genotyp * MeSH
• haplotypy MeSH
• hlavní histokompatibilní komplex genetika   MeSH
• kur domácí MeSH
• polymorfismus délky restrikčních fragmentů MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

We report that the cloned DNA harboring the long terminal repeat (LTR), v-src, LTR proviral structure is tumorigenic in chickens of the Prague congenic lines. The growth rate of these tumors is by far the highest in the recombinant CC.R1 line, the B haplotype of which is composed of the B-F/L4 and B-G12 subregions originating from different naturally occurring haplotypes. Some of the tumors induced by the LTR, v-src, LTR DNA are repeatedly transplantable in syngeneic chickens, maintain unaltered provirus, and express v-src mRNA. Differences in the response to challenge with Rous sarcoma virus (RSV) and LTR, v-src, LTR DNA on a given experimental model are compared and possible involvement of an interaction between B-F/L and B-G region genes is considered. Regression of the LTR, v-src, LTR DNA-induced tumors did not prevent the formation and growth of tumors induced subsequently by RSV.

• MeSH
• buněčné dělení genetika   MeSH
• DNA nádorová fyziologie   MeSH
• geny src fyziologie   MeSH
• klonování DNA MeSH
• kur domácí MeSH
• nádory genetika   mikrobiologie   MeSH
• northern blotting MeSH
• polymorfismus délky restrikčních fragmentů MeSH
• proviry MeSH
• repetitivní sekvence nukleových kyselin fyziologie   MeSH
• Southernův blotting MeSH
• testy genetické komplementace MeSH
• viry ptačího sarkomu MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• DNA nádorová MeSH