Strigolactones are a class of phytohormones with various functions in plant development, stress responses, and in the interaction with (micro)organisms in the rhizosphere. While their effects on vegetative development are well studied, little is known about their role in reproduction. We investigated the effects of genetic and chemical modification of strigolactone levels on the timing and intensity of flowering in tomato (Solanum lycopersicum L.) and the molecular mechanisms underlying such effects. Results showed that strigolactone levels in the shoot, whether endogenous or exogenous, correlate inversely with the time of anthesis and directly with the number of flowers and the transcript levels of the florigen-encoding gene SINGLE FLOWER TRUSS (SFT) in the leaves. Transcript quantifications coupled with metabolite analyses demonstrated that strigolactones promote flowering in tomato by inducing the activation of the microRNA319-LANCEOLATE module in leaves. This, in turn, decreases gibberellin content and increases the transcription of SFT. Several other floral markers and morpho-anatomical features of developmental progression are induced in the apical meristems upon treatment with strigolactones, affecting floral transition and, more markedly, flower development. Thus, strigolactones promote meristem maturation and flower development via the induction of SFT both before and after floral transition, and their effects are blocked in plants expressing a miR319-resistant version of LANCEOLATE. Our study positions strigolactones in the context of the flowering regulation network in a model crop species.

• Klíčová slova
• LANCEOLATE, flowering, miR319, strigolactones, tomato,
• MeSH
• gibereliny metabolismus   farmakologie   MeSH
• květy * účinky léků   růst a vývoj   metabolismus   genetika   MeSH
• laktony * metabolismus   farmakologie   MeSH
• listy rostlin metabolismus   účinky léků   MeSH
• mikro RNA * genetika   metabolismus   MeSH
• regulace genové exprese u rostlin * účinky léků   MeSH
• regulátory růstu rostlin metabolismus   farmakologie   MeSH
• rostlinné proteiny metabolismus   genetika   MeSH
• Solanum lycopersicum * genetika   růst a vývoj   metabolismus   účinky léků   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• gibereliny MeSH
• laktony * MeSH
• mikro RNA * MeSH
• regulátory růstu rostlin MeSH
• rostlinné proteiny MeSH

BACKGROUND: Drought is a major environmental stress that affects crop productivity worldwide. Although previous research demonstrated links between strigolactones (SLs) and drought, here we used barley (Hordeum vulgare) SL-insensitive mutant hvd14 (dwarf14) to scrutinize the SL-dependent mechanisms associated with water deficit response. RESULTS: We have employed a combination of transcriptomics, proteomics, phytohormonomics analyses, and physiological data to unravel differences between wild-type and hvd14 plants under drought. Our research revealed that drought sensitivity of hvd14 is related to weaker induction of abscisic acid-responsive genes/proteins, lower jasmonic acid content, higher reactive oxygen species content, and lower wax biosynthetic and deposition mechanisms than wild-type plants. In addition, we identified a set of transcription factors (TFs) that are exclusively drought-induced in the wild-type barley. CONCLUSIONS: Critically, we resolved a comprehensive series of interactions between the drought-induced barley transcriptome and proteome responses, allowing us to understand the profound effects of SLs in alleviating water-limiting conditions. Several new avenues have opened for developing barley more resilient to drought through the information provided. Moreover, our study contributes to a better understanding of the complex interplay between genes, proteins, and hormones in response to drought, and underscores the importance of a multidisciplinary approach to studying plant stress response mechanisms.

• Klíčová slova
• Abscisic acid, Barley (Hordeum vulgare), Drought, Phytohormone, Proteome, Strigolactone, Transcriptome,
• MeSH
• ječmen (rod) * genetika   MeSH
• multiomika MeSH
• období sucha MeSH
• percepce MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• GR24 strigolactone MeSH Prohlížeč