Plant reactions to stress vary with development stage and fitness. This study assessed the relationship between light and chilling stress in Arabidopsis acclimation. By analysing the transcriptome and proteome responses of expanding leaves subjected to varying light intensity and cold, 2251 and 2064 early response genes and proteins were identified, respectively. Many of these represent as a yet unknown part of the early response to cold, illustrating a development-dependent response to stress and duality in plant adaptations. While standard light promoted photosynthetic upregulation, plastid maintenance, and increased resilience, low light triggered a unique metabolic shift, prioritizing ribosome biogenesis and lipid metabolism and attenuating the expression of genes associated with plant immunity. The comparison of early response in young leaves with that in expanded ones showed striking differences, suggesting a sacrifice of expanded leaves to support young ones. Validations of selected DEGs in mutant background confirmed a role of HSP90-1, transcription factor FLZ13, and Phospholipase A1 (PLIP) in response to cold, and the PLIP family emerged as crucial in promoting acclimation and freezing stress tolerance. The findings highlight the dynamic mechanisms that enable plants to adapt to challenging environments and pave the way for the development of genetically modified crops with enhanced freezing tolerance.

• Klíčová slova
• acclimation, freezing tolerance, jasmonic acid, leaf development, lipidome, proteome, transcriptome,
• MeSH
• aklimatizace * fyziologie   účinky záření   MeSH
• Arabidopsis * fyziologie   účinky záření   genetika   MeSH
• fotosyntéza MeSH
• listy rostlin * fyziologie   účinky záření   genetika   MeSH
• nízká teplota * MeSH
• proteiny huseníčku metabolismus   genetika   MeSH
• proteom MeSH
• regulace genové exprese u rostlin MeSH
• světlo * MeSH
• transkriptom MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• proteiny huseníčku MeSH
• proteom MeSH

The reproductive stage of plant development has the most critical impact on yield. Flowering is highly sensitive to abiotic stress, and increasing temperatures and drought harm crop yields. Salicylic acid is a phytohormone that regulates flowering and promotes stress resilience in plants. However, the exact molecular mechanisms and the level of protection are far from understood and seem to be species-specific. Here, the effect of salicylic acid was tested in a field experiment with Pisum sativum exposed to heat stress. Salicylic acid was administered at two different stages of flowering, and its effect on the yield and composition of the harvested seeds was followed. Plants treated with salicylic acid produced larger seed pods, and a significant increase in dry weight was found for the plants with a delayed application of salicylic acid. The analyses of the seed proteome, lipidome, and metabolome did not show any negative impact of salicylic treatment on seed composition. Identified processes that could be responsible for the observed improvement in seed yields included an increase in polyamine biosynthesis, accumulation of storage lipids and lysophosphatidylcholines, a higher abundance of components of chromatin regulation, calmodulin-like protein, and threonine synthase, and indicated a decrease in sensitivity to abscisic acid signaling.

• Klíčová slova
• field, heat stress, lipidome, metabolome, phytohormone, proteome, seed development, stress attenuation, yield,
• MeSH
• fyziologický stres MeSH
• hrách setý * metabolismus   MeSH
• kyselina salicylová * farmakologie   metabolismus   MeSH
• rostliny metabolismus   MeSH
• semena rostlinná metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• kyselina salicylová * MeSH