The bean bug (Riptortus pedestris) is a pest of soybeans and other legumes in Japan and other Asian countries. It enters a facultative adult diapause on exposure to short days. While photoperiodism and diapause are well understood in R. pedestris, knowledge of cold tolerance is very limited, as is information on the effect of diapause on cold tolerance. We examined the effect of photoperiod, cold acclimation, and feeding status on cold tolerance in R. pedestris. We found that cold acclimation significantly increased survival at -10°C in both long- and short-day adult R. pedestris. Since the difference in cold survival between long- and short-day cold-acclimated groups was only marginal, we conclude that entering diapause is not crucial for R. pedestris to successfully pass through cold acclimation and become cold tolerant. We observed similar effects in 5th instar nymphs, with both long- and short-day cold-acclimated groups surviving longer cold exposures compared with non-acclimated groups. Starvation, which was tested only in adult bugs, had only a negligible and negative impact on cold survival. Although cold tolerance significantly increased with cold acclimation in adult bugs, supercooling capacity unexpectedly decreased. Our results suggest that changes in supercooling capacity as well as in water content are unrelated to cold tolerance in R. pedestris. An analysis of metabolites revealed differences between the treatments, and while several metabolites markedly increased with cold acclimation, their concentrations were too low to have a significant effect on cold tolerance.

• Klíčová slova
• Riptortus pedestris, cold acclimation, cold tolerance, diapause,
• MeSH
• aklimatizace * fyziologie   MeSH
• diapauza hmyzu fyziologie   MeSH
• Heteroptera metabolismus   fyziologie   MeSH
• metabolomika MeSH
• nízká teplota škodlivé účinky   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

Phenotypic plastic responses to temperature can modulate the kinetic effects of temperature on biological rates and traits and thus play an important role for species adaptation to climate change. However, there is little information on how these plastic responses to temperature can influence trophic interactions. Here, we conducted an experiment using marbled crayfish and their water louse prey to investigate how short-term thermal acclimation at two temperatures (16 and 24°C) modulates the predator functional response. We found that both functional response parameters (search rate and handling time) differed between the two experimental temperatures. However, the sign and magnitudes of these differences strongly depended on acclimation time. Acclimation to 16°C increased handling time and search rate whereas acclimation to 24°C leads to the opposite effects with shorter handling time and lower search rate for acclimated predators. Moreover, the strength of these effects increased with acclimation time so that the differences in search rate and handing time between the two temperatures were reversed between the treatment without acclimation and after 24 h of acclimation. Overall, we found that the magnitude of the acclimation effects can be as strong as the direct kinetic effects of temperature. Our study highlights the importance of taking into account short-term thermal plasticity to improve our understanding of the potential consequences of global warming on species interactions.

• Klíčová slova
• acclimation, functional response, metabolic theory, temperature,
• Publikační typ
• časopisecké články MeSH

Cold tolerance is a key determinant of poleward colonization in insects. However, the physiological basis underlying interspecific differences in cold tolerance is not fully understood. Here, we analyzed cold tolerance and metabolomic profiles in warm- and cold-acclimated phenotypes of 43 Drosophila species representing a latitudinal gradient from the tropics to the boreal zone. We found a strong positive correlation between cold tolerance and climatic variables associated with habitat seasonality and temperature. Including the effects of cold acclimation, we found most species have similar 'safety margins', measured as the difference between the average environmental temperature and the lower lethal temperature. Searching for metabolomic signatures of cold tolerance, we found that the warm-acclimated flies of cold-hardy species had moderately but significantly higher constitutive signals of putative cryoprotectants such as trehalose, glucose, glycerol and mannitol/sorbitol. Cold acclimation (and the transition to a winter dormant phenotype) resulted in a strong accumulation of myo-inositol, which occurred only in species of the virilis group. Other temperate and boreal species either showed only moderate, idiosyncratic accumulations of sugars/polyols and free amino acids, or did not accumulate any 'classical' cryoprotectant at all. Thus, our results suggest that the colonization of boreal regions by Drosophila does not necessarily depend on the seasonal accumulation of classical cryoprotectants. In contrast, virtually all cold-acclimated species showed a significant increase in products of phospholipid catabolism, suggesting that remodeling of biological membranes is a clear and ubiquitous signature of cold acclimation in Drosophila.

• Klíčová slova
• Acclimation, Climatic zones, Cold tolerance, Cryoprotectants, Insects, Metabolomics,
• MeSH
• aklimatizace * fyziologie   MeSH
• Drosophila * fyziologie   metabolismus   MeSH
• druhová specificita MeSH
• kryoprotektivní látky metabolismus   MeSH
• metabolom MeSH
• metabolomika MeSH
• nízká teplota * MeSH
• roční období * MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• kryoprotektivní látky MeSH

Cold acclimation and vernalization represent the major evolutionary adaptive responses to ensure winter survival of temperate plants. Due to climate change, mild winters can paradoxically worsen plant winter survival due to cold deacclimation induced by warm periods during winter. It seems that the ability of cold reacclimation in overwintering Triticeae cereals is limited, especially in vernalized plants. In the present review, the major factors determining cold acclimation (CA), deacclimation (DA) and reacclimation (RA) processes in winter-type Triticeae, namely wheat and barley, are discussed. Recent knowledge on cold sensing and signaling is briefly summarized. The impacts of chilling temperatures, photoperiod and light spectrum quality as the major environmental factors, and the roles of soluble proteins and sugars (carbohydrates) as well as cold stress memory molecular mechanisms as the major plant-based factors determining CA, DA, and RA processes are discussed. The roles of plant stress memory mechanisms and development processes, namely vernalization, in winter Triticeae reacclimation are elucidated. Recent findings about the role of O-glucose N-acetylation of target proteins during vernalization and their impacts on the expression of VRN1 gene and other target proteins resulting in cold-responsive modules reprogramming are presented.

• Klíčová slova
• Cold acclimation, Deacclimation, Light quality, Photoperiod, Reacclimation, Triticeae, Vernalization,
• MeSH
• aklimatizace * fyziologie   MeSH
• ječmen (rod) * fyziologie   metabolismus   MeSH
• nízká teplota * MeSH
• pšenice * fyziologie   metabolismus   MeSH
• roční období * MeSH
• rostlinné proteiny metabolismus   genetika   MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• rostlinné proteiny MeSH

Cold exposure depolarizes cells in insects due to a reduced electrogenic ion transport and a gradual increase in extracellular K+ concentration ([K+]). Cold-induced depolarization is linked to cold injury in chill-susceptible insects, and the locust, Locusta migratoria, has been shown to improve cold tolerance following cold acclimation through depolarization resistance. Here we investigate how cold acclimation influences depolarization resistance and how this resistance relates to improved cold tolerance. To address this question, we investigated if cold acclimation affects the electrogenic transport capacity and/or the relative K+ permeability during cold exposure by measuring membrane potentials of warm- and cold-acclimated locusts in the presence and absence of ouabain (Na+-K+ pump blocker) or 4-aminopyridine (4-AP; voltage-gated K+ channel blocker). In addition, we compared the membrane lipid composition of muscle tissue from warm- and cold-acclimated locust and the abundance of a range transcripts related to ion transport and cell injury accumulation. We found that cold-acclimated locusts are depolarization resistant due to an elevated K+ permeability, facilitated by opening of 4-AP-sensitive K+ channels. In accordance, cold acclimation was associated with an increased abundance of Shaker transcripts (gene encoding 4-AP-sensitive voltage-gated K+ channels). Furthermore, we found that cold acclimation improved muscle cell viability following exposure to cold and hyperkalemia even when muscles were depolarized substantially. Thus cold acclimation confers resistance to depolarization by altering the relative ion permeability, but cold-acclimated locusts are also more tolerant to depolarization.

• Klíčová slova
• acclimation, cold exposure, depolarization, insect,
• MeSH
• 4-aminopyridin farmakologie   MeSH
• aklimatizace účinky léků   fyziologie   MeSH
• kosterní svalová vlákna účinky léků   fyziologie   MeSH
• Locusta migratoria fyziologie   MeSH
• membránové potenciály účinky léků   fyziologie   MeSH
• nízká teplota * MeSH
• ouabain farmakologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• 4-aminopyridin MeSH
• ouabain MeSH

Many temperate insects encounter temperatures low enough to freeze their body fluids. Remarkably, some insects are freeze-tolerant, surviving this internal ice formation. However, the mechanisms underlying freeze tolerance are not well-understood, in part due to a lack of tractable model organisms. We describe a novel laboratory model to study insect freeze tolerance, the spring field cricket Gryllus veletis (Orthopera: Gryllidae). Following acclimation to six weeks of decreasing temperature and photoperiod, G. veletis become freeze-tolerant, similar to those exposed to natural autumn conditions in London, Ontario, Canada. Acclimated crickets suppress their metabolic rate by c. 33%, and survive freezing for up to one week at -8 °C, and to temperatures as low as -12 °C. Freeze-tolerant G. veletis protect fat body cells from freeze injury in vivo, and fat body tissue from freeze-tolerant cricket survives brief freeze treatments when frozen ex vivo. Freeze-tolerant crickets freeze at c. -6 °C, which may be initiated by accumulation of ice-nucleating agents in hemolymph or gut tissue. Although we hypothesize that control of ice formation facilitates freeze tolerance, initiating ice formation at high subzero temperatures does not confer freeze tolerance on freeze-intolerant nymphs. Acclimation increases hemolymph osmolality from c. 400 to c. 650 mOsm, which may facilitate freeze tolerance by reducing ice content. Hemolymph ion concentrations do not change with acclimation, and we therefore predict that freeze-tolerant G. veletis elevate hemolymph osmolality by accumulating other molecules. Gryllus veletis is easily reared and manipulated in a controlled laboratory environment, and is therefore a suitable candidate for further investigating the mechanisms underlying freeze tolerance.

• Klíčová slova
• Acclimation, Cold tolerance, Freeze tolerance, Ice nucleation, Insect, Metabolic rate,
• MeSH
• aklimatizace * MeSH
• Gryllidae růst a vývoj   fyziologie   MeSH
• homeostáza MeSH
• nízká teplota * MeSH
• nymfa růst a vývoj   fyziologie   MeSH
• roční období MeSH
• zmrazování * MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Ontario MeSH

Heat stress is a frequent environmental constraint. Phytohormones can significantly affect plant thermotolerance. This study compares the effects of exogenous cytokinin meta-topolin-9-(tetrahydropyran-2-yl)purine (mT9THP) on rice (Oryza sativa) under control conditions, after acclimation by moderate temperature (A; 37 °C, 2h), heat stress (HS; 45 °C, 6h) and their combination (AHS). mT9THP is a stable cytokinin derivative that releases active meta-topolin gradually, preventing the rapid deactivation reported after exogenous cytokinin application. Under control conditions, mT9THP negatively affected jasmonic acid in leaves and abscisic and salicylic acids in crowns (meristematic tissue crucial for tillering). Exogenous cytokinin stimulated the emission of volatile organic compounds (VOC), especially 2,3-butanediol. Acclimation upregulated trans-zeatin, expression of stress- and hormone-related genes, and VOC emission. The combination of acclimation and mT9THP promoted the expression of stress markers and antioxidant enzymes and moderately increased VOC emission, including 2-ethylhexyl salicylate or furanones. AHS and HS responses shared some common features, namely, increase of ethylene precursor aminocyclopropane-1-carboxylic acid (ACC), cis-zeatin and cytokinin methylthio derivatives, as well as the expression of heat shock proteins, alternative oxidases, and superoxide dismutases. AHS specifically induced jasmonic acid and auxin indole-3-acetic acid levels, diacylglycerolipids with fewer double bonds, and VOC emissions [e.g., acetamide, lipoxygenase (LOX)-derived volatiles]. Under direct HS, exogenous cytokinin mimicked some positive acclimation effects. The combination of mT9THP and AHS had the strongest thermo-protective effect, including a strong stimulation of VOC emissions (including LOX-derived ones). These results demonstrate for the first time the crucial contribution of volatiles to the beneficial effects of cytokinin and AHS on rice thermotolerance.

• Klíčová slova
• Acclimation, Cytokinin, Heat stress, Phytohormone, Priming, Thermotolerance, Volatile organic compound,
• MeSH
• aklimatizace MeSH
• cytokininy metabolismus   MeSH
• exprese genu MeSH
• rýže (rod) * metabolismus   MeSH
• těkavé organické sloučeniny * MeSH
• termotolerance * MeSH
• zeatin metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• cytokininy MeSH
• jasmonic acid MeSH Prohlížeč
• těkavé organické sloučeniny * MeSH
• zeatin MeSH

It has previously been shown that the long-term treatment of Arabidopsis thaliana with the chloroplast inhibitor lincomycin leads to photosynthetic membranes enriched in antennas, strongly reduced in photosystem II reaction centers (PSII) and with enhanced nonphotochemical quenching (NPQ) (Belgio et al. Biophys J 102:2761-2771, 2012). Here, a similar physiological response was found in the microalga Chromera velia grown under high light (HL). In comparison to cells acclimated to low light, HL cells displayed a severe re-organization of the photosynthetic membrane characterized by (1) a reduction of PSII but similar antenna content; (2) partial uncoupling of antennas from PSII; (3) enhanced NPQ. The decrease in the number of PSII represents a rather unusual acclimation response compared to other phototrophs, where a smaller PSII antenna size is more commonly found under high light. Despite the diminished PSII content, no net damage could be detected on the basis of the Photosynthesis versus irradiance curve and electron transport rates pointing at the excess capacity of PSII. We therefore concluded that the photoinhibition is minimized under high light by a lower PSII content and that cells are protected by NPQ in the antennas.

• Klíčová slova
• Chromera velia alga, High light acclimation, Nonphotochemical quenching, Photoinhibition, Uncoupling of antennas from Photosystem II.,
• MeSH
• aklimatizace účinky záření   MeSH
• Alveolata cytologie   fyziologie   účinky záření   MeSH
• chlorofyl a MeSH
• chlorofyl metabolismus   MeSH
• fluorescence MeSH
• fotochemické procesy účinky záření   MeSH
• fotosyntéza účinky záření   MeSH
• fotosystém II (proteinový komplex) metabolismus   MeSH
• rozpustnost MeSH
• světlo * MeSH
• světlosběrné proteinové komplexy metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• chlorofyl a MeSH
• chlorofyl MeSH
• fotosystém II (proteinový komplex) MeSH
• světlosběrné proteinové komplexy MeSH

Seasonal acclimation and thermoregulation represent major components of complex thermal strategies by which ectotherms cope with the heterogeneity of their thermal environment. Some ectotherms possess the acclimatory capacity to shift seasonally their thermoregulatory behavior, but the frequent use of constant acclimation temperatures during experiments and the lack of information about thermal heterogeneity in the field obscures the ecological relevance of this plastic response. We examined the experimentally induced seasonal acclimation of preferred body temperatures (T(p)) in alpine newts Ichthyosaura (formerly Triturus) alpestris subjected to a gradual increase in acclimation temperature from 5°C during the winter to a constant 15°C or diel fluctuations between 10° and 20°C during the spring/summer. Both the mean and range of T(p) followed the increase in mean acclimation temperature without the influence of diel temperature fluctuations. The direction and magnitude of this acclimatory capacity has the potential to increase the time window available for thermoregulation. Although thermoregulation and thermal acclimation are often considered as separate but coadapted adjustments to thermal heterogeneity, their combined response is employed by newts to tackle seasonal variation in a thermoregulatory-challenging aquatic environment.

• MeSH
• aklimatizace * MeSH
• chování zvířat fyziologie   MeSH
• roční období * MeSH
• Salamandridae fyziologie   MeSH
• teplota MeSH
• termoregulace fyziologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Plant survival in temperate zones requires efficient cold acclimation, which is strongly affected by light and temperature signal crosstalk, which converge in modulation of hormonal responses. Cold under low light conditions affected Arabidopsis responses predominantly in apices, possibly because energy supplies were too limited for requirements of these meristematic tissues, despite a relatively high steady-state quantum yield. Comparing cold responses at optimal light intensity and low light, we found activation of similar defence mechanisms-apart from CBF1-3 and CRF3-4 pathways, also transient stimulation of cytokinin type-A response regulators, accompanied by fast transient increase of trans-zeatin in roots. Upregulated expression of components of strigolactone (and karrikin) signalling pathway indicated involvement of these phytohormones in cold responses. Impaired response of phyA, phyB, cry1 and cry2 mutants reflected participation of these photoreceptors in acquiring freezing tolerance (especially cryptochrome CRY1 at optimal light intensity and phytochrome PHYA at low light). Efficient cold acclimation at optimal light was associated with upregulation of trans-zeatin in leaves and roots, while at low light, cytokinin (except cis-zeatin) content remained diminished. Cold stresses induced elevation of jasmonic acid and salicylic acid (in roots). Low light at optimal conditions resulted in strong suppression of cytokinins, jasmonic and salicylic acid.

• Klíčová slova
• auxin, combined stress, cryptochrome, cytokinin, gene expression, gibberellin, phytochrome, plant hormones,
• MeSH
• aklimatizace * MeSH
• Arabidopsis * genetika   metabolismus   MeSH
• proteiny huseníčku * biosyntéza   genetika   MeSH
• regulace genové exprese u rostlin * MeSH
• světlo * MeSH
• zmrazování * MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• proteiny huseníčku * MeSH