Cannabidiol (CBD), a phytocannabinoid commonly isolated from chemotype III Cannabis sativa plants, is known for its therapeutic potential. However, comprehensive information on its bioavailability is still lacking. The key objective of this study was to investigate the impact of specific formulations on CBD delivery to the site of action and, in particular, the brain of experimental animals. As brain tissue is an extremely complex matrix, a highly sensitive method employing liquid chromatography-tandem mass spectrometry (LC-MS/MS) had to be implemented. To make it applicable for multiple analytes, the method was validated for 17 other phytocannabinoids and selected metabolites. Using this method, a pharmacokinetic study was conducted on 200 brain samples collected from rats that had been administered various CBD formulations (carriers) via oral gavage. The peak concentration in brain occurred within 1-2 h; notably, the highest was reached with carriers containing triacylglycerols with the shortest fatty acid chains (caprylic/capric). In addition to the parent compound, 7-hydroxy-cannabidiol and 7-carboxy-cannabidiol were detected, confirming rapid post-administration metabolism. Overall, this research enhances understanding of CBD distribution in the brain and underscores the impact of specific formulations on its bioavailability, offering insights into optimizing CBD-based therapies to be both effective and 'patient-friendly'.

• Klíčová slova
• Cannabidiol, LC-MS/MS, bioavailability, brain tissue, metabolites,
• MeSH
• aplikace orální MeSH
• biologická dostupnost MeSH
• chromatografie kapalinová MeSH
• kanabidiol * farmakokinetika   aplikace a dávkování   chemie   MeSH
• krysa rodu Rattus MeSH
• mozek * metabolismus   účinky léků   MeSH
• tandemová hmotnostní spektrometrie MeSH
• tkáňová distribuce MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• kanabidiol * MeSH

Besides many other uses, dried Cannabis may be used for "tea" preparation. This study focused on a comprehensive characterization of an aqueous infusion prepared according to a common practice from three fairly different Cannabis cultivars. The transfer of 42 phytocannabinoids and 12 major bioactive compounds (flavonoids) into the infusion was investigated using UHPLC-HRMS/MS. Phytocannabinoid acids were transferred generally in a higher extent compared to their counterparts; in the case of Δ9-THC, it was only in the range of 0.4-1.9% of content in the Cannabis used. A dramatic increase of phytocannabinoids, mainly of the neutral species, occurred when cream was added during steeping, and the transfer of Δ9-THC into "tea" achieved a range of 53-64%. Under such conditions, drinking a 250 mL cup of such tea by a 70 kg person might lead to multiple exceedance of the Acute Reference Dose (ARfD), 1 μg/kg b.w., even in the case when using hemp with a Δ9-THC content below 1% in dry weight for preparation.

• Klíčová slova
• THC isomers, UHPLC-HRMS/MS, bioactive compounds, cannabis tea, cannflavins, flavonoids, phytocannabinoids,
• MeSH
• čaj chemie   MeSH
• Cannabis * chemie   MeSH
• flavonoidy chemie   analýza   MeSH
• kanabinoidy analýza   chemie   MeSH
• lidé MeSH
• rostlinné extrakty chemie   MeSH
• tandemová hmotnostní spektrometrie MeSH
• tetrahydrokanabinol analýza   chemie   MeSH
• vysokoúčinná kapalinová chromatografie MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• čaj MeSH
• flavonoidy MeSH
• kanabinoidy MeSH
• rostlinné extrakty MeSH
• tetrahydrokanabinol MeSH