After growth on sucrose or glucose, Endomyces magnusii possess a monosaccharide uptake which resembles that of Saccharomyces cerevisiae (a high KT of uptake, preference for alpha-anomers of D-xylose and D-glucose, enhanced uptake during anaerobiosis, attainment of a diffusion equilibrium). The uptake is inhibited by other monosaccharides and especially strongly by D-galactose. In the absence of high concentrations of metabolizable sugars. E. magnusii develops a capacity to accumulate 3-O-methyl-D-glucose and D-xylose against a concentration gradient the new system displaying a high affinity for glucose (KT less than 0.1 mM), repression by glucose, mannose or galactose. Cycloheximide (0.2%) blocks the formation of the active system.

• MeSH
• Ascomycota metabolism   MeSH
• Galactose pharmacology   MeSH
• Glucose metabolism   MeSH
• Mannose pharmacology   MeSH
• Monosaccharides metabolism   MeSH
• Ribose pharmacology   MeSH
• Saccharomycetales metabolism   MeSH
• Sorbose pharmacology   MeSH
• Xylose metabolism   pharmacology   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Galactose MeSH
• Glucose MeSH
• Mannose MeSH
• Monosaccharides MeSH
• Ribose MeSH
• Sorbose MeSH
• Xylose MeSH

Tritium-labelled 4-deoxy-D-glucose (4-dglc) and 6-deoxy-D-glucose (6-dgcl) were prepared by catalytic hydrogenolysis of the corresponding deoxyiodo derivatives with gaseous tritium. The two sugars are transported into Saccharomyces cerevisiae by both the constitutive glucose and the inducible galactose carrier. Uranyl ions are powerful inhibitors. The pH optimum in uninduced cells lies at 5.5 for both sugars, the apparent activation energies (between 15 and 35 degrees C) are 25.1 kJ/mol and 16.5 kJ/mol, respectively. The steady-state intracellular concentration of both sugars is less than the extracellular one (no uphill transport). Neither of them is a substrate of yeast hexokinase. 4-Deoxy-D-glucose undergoes a dinitrophenol-sensitive conversion to an unknown metabolite which is not phosphorylated and may represent one of its oxidation products.

• MeSH
• Deoxy Sugars metabolism   MeSH
• Deoxyglucose chemical synthesis   metabolism   MeSH
• Dinitrophenols pharmacology   MeSH
• Fructose metabolism   MeSH
• Glucose metabolism   MeSH
• Hexokinase metabolism   MeSH
• Iodoacetamide pharmacology   MeSH
• Hydrogen-Ion Concentration MeSH
• Saccharomyces cerevisiae enzymology   metabolism   MeSH
• Temperature MeSH
• Electron Transport MeSH
• Uranium pharmacology   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Deoxy Sugars MeSH
• Deoxyglucose MeSH
• Dinitrophenols MeSH
• Fructose MeSH
• Glucose MeSH
• Hexokinase MeSH
• Iodoacetamide MeSH
• Uranium MeSH

• MeSH
• Acetates metabolism   MeSH
• Biological Transport, Active MeSH
• Arabinose metabolism   MeSH
• Cell Membrane metabolism   MeSH
• Ethanol metabolism   MeSH
• Phosphates metabolism   MeSH
• Glucose metabolism   MeSH
• Phosphorus Isotopes MeSH
• Carbon Isotopes MeSH
• Hydrogen-Ion Concentration MeSH
• Culture Media MeSH
• Lactates metabolism   MeSH
• Lactose metabolism   MeSH
• Maltose metabolism   MeSH
• Carbohydrate Metabolism * MeSH
• Protoplasts metabolism   MeSH
• Ribose metabolism   MeSH
• Saccharomyces cerevisiae metabolism   MeSH
• Sorbose metabolism   MeSH
• Oxygen Consumption MeSH
• Xylose metabolism   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Acetates MeSH
• Arabinose MeSH
• Ethanol MeSH
• Phosphates MeSH
• Glucose MeSH
• Phosphorus Isotopes MeSH
• Carbon Isotopes MeSH
• Culture Media MeSH
• Lactates MeSH
• Lactose MeSH
• Maltose MeSH
• Ribose MeSH
• Sorbose MeSH
• Xylose MeSH

• MeSH
• Acetamides pharmacology   MeSH
• Acetates pharmacology   MeSH
• Amides pharmacology   MeSH
• Amino Acids metabolism   MeSH
• Anaerobiosis MeSH
• Azides pharmacology   MeSH
• Cycloheximide pharmacology   MeSH
• Dinitrophenols pharmacology   MeSH
• Fluorides pharmacology   MeSH
• Glycine antagonists & inhibitors   metabolism   MeSH
• Iodoacetates pharmacology   MeSH
• Caproates pharmacology   MeSH
• Hydrogen-Ion Concentration MeSH
• Aspartic Acid antagonists & inhibitors   metabolism   MeSH
• Lysine antagonists & inhibitors   metabolism   MeSH
• Saccharomyces cerevisiae drug effects   MeSH
• Saccharomyces drug effects   metabolism   MeSH
• Uranium pharmacology   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Acetamides MeSH
• Acetates MeSH
• Amides MeSH
• Amino Acids MeSH
• Azides MeSH
• Cycloheximide MeSH
• Dinitrophenols MeSH
• Fluorides MeSH
• Glycine MeSH
• Iodoacetates MeSH
• Caproates MeSH
• Aspartic Acid MeSH
• Lysine MeSH
• Uranium MeSH

• MeSH
• Aerobiosis MeSH
• Nitrates pharmacology   MeSH
• Hexoses metabolism   MeSH
• Carbon Isotopes MeSH
• Culture Media MeSH
• Pentoses metabolism   MeSH
• Cell Membrane Permeability drug effects   MeSH
• Saccharomyces drug effects   growth & development   metabolism   MeSH
• Thorium pharmacology   MeSH
• Electron Transport drug effects   MeSH
• Uranium pharmacology   MeSH
• Xylose metabolism   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Nitrates MeSH
• Hexoses MeSH
• Carbon Isotopes MeSH
• Culture Media MeSH
• Pentoses MeSH
• Thorium MeSH
• Uranium MeSH
• Xylose MeSH

• MeSH
• Arabinose pharmacology   MeSH
• Biological Transport drug effects   MeSH
• Glucose metabolism   pharmacology   MeSH
• Carbon Isotopes MeSH
• Yeasts metabolism   MeSH
• Ribose metabolism   pharmacology   MeSH
• Xylose pharmacology   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Arabinose MeSH
• Glucose MeSH
• Carbon Isotopes MeSH
• Ribose MeSH
• Xylose MeSH

• MeSH
• Arabinose metabolism   MeSH
• Densitometry MeSH
• Dinitrophenols pharmacology   MeSH
• Fructose metabolism   MeSH
• Glucose metabolism   MeSH
• Iodoacetates pharmacology   MeSH
• Copper pharmacology   MeSH
• Monosaccharides metabolism   MeSH
• Protoplasts metabolism   MeSH
• Rhamnose metabolism   MeSH
• Ribose metabolism   MeSH
• Saccharomyces drug effects   metabolism   MeSH
• Uranium pharmacology   MeSH
• Xylose metabolism   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Arabinose MeSH
• Dinitrophenols MeSH
• Fructose MeSH
• Glucose MeSH
• Iodoacetates MeSH
• Copper MeSH
• Monosaccharides MeSH
• Rhamnose MeSH
• Ribose MeSH
• Uranium MeSH
• Xylose MeSH

• MeSH
• Biological Transport, Active * MeSH
• Amides pharmacology   MeSH
• Arabinose metabolism   MeSH
• Candida drug effects   metabolism   MeSH
• Dinitrophenols pharmacology   MeSH
• Fluorides pharmacology   MeSH
• Glucose metabolism   MeSH
• Kinetics MeSH
• Hydrogen-Ion Concentration MeSH
• Monosaccharides metabolism   MeSH
• Rhamnose metabolism   MeSH
• Ribose metabolism   MeSH
• Temperature MeSH
• Uranium pharmacology   MeSH
• Xylose metabolism   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Amides MeSH
• Arabinose MeSH
• Dinitrophenols MeSH
• Fluorides MeSH
• Glucose MeSH
• Monosaccharides MeSH
• Rhamnose MeSH
• Ribose MeSH
• Uranium MeSH
• Xylose MeSH