Polysaccharide hydrolase activity was assayed in a group of 28 selected Rhizopus strains. The production of lichenases, mannanases, cellulases, xylanases, amylases and pullulanases was demonstrated using the gel-testing method during growth of the strains on suitably meshed polysaccharide gels.

• MeSH
• Fermentation MeSH
• Polysaccharides metabolism   MeSH
• Rhizopus enzymology   MeSH
• Xylosidases metabolism   MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Polysaccharides MeSH
• Xylosidases MeSH

The polysaccharide hydrolase activity of a group of selected strains of the genus Aureobasidium pullulans was investigated using a new gel testing assay. A total of 31 strains were tested for alpha-amylase, alpha-glucosidase and glucoamylase, beta-glucosidase, lichenase, cellulase, xylanase and xylosidase, mannanase and mannosidase production during growth of microorganisms on respective meshed polysaccharide gels. Attempts were made to increase the polysaccharide hydrolase activity through selection of some A. pullulans strains by passaging them on the respective modified xylanase- and cellulase-containing gels. The individual saccharide degradation cleavage products were investigated by chromatography.

• MeSH
• alpha-Amylases metabolism   MeSH
• Cellulase metabolism   MeSH
• Xylan Endo-1,3-beta-Xylosidase MeSH
• Chromatography, Gel MeSH
• Hydrolases metabolism   MeSH
• Mitosporic Fungi enzymology   MeSH
• Polysaccharides metabolism   MeSH
• Xylosidases metabolism   MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• alpha-Amylases MeSH
• Cellulase MeSH
• Xylan Endo-1,3-beta-Xylosidase MeSH
• Hydrolases MeSH
• Polysaccharides MeSH
• Xylosidases MeSH