The proportion of fucosylated glycoconjugate-containing rabbit tracheal goblet cells after intratracheal application of trimecaine was studied to evaluate its possible unfavourable effects. This lapine model is comparable with diagnostic findings in humans because airway epithelia in humans and rabbits are similar; tracheal epithelium is also practically identical to bronchial epithelium in both species. Local trimecaine anaesthesia caused a proportional decrease in percentage of the tracheal goblet cells containing both alpha(1-2)- and alpha(1-6)-, alpha(1-3)- and alpha(1-4)-fucosylated glycoconjugates as revealed 10 min postexposure using lectin histochemistry. In previous studies, only mild ultrastructural damage to the airway's epithelium was revealed, but a conspicuous decrease in sialylated glycoconjugate-containing tracheal goblet cells and the dominance of acidic sulphated glycoconjugates were observed as after-effects of the same treatment. Glycoconjugate changes can influence the inner environment of airways (e.g. viscoelastic properties of the airways' mucus and mucosal barrier functions) and thus the patient's defence barriers in airways may be weakened. Concurrently, the histochemical properties of goblet cells can be altered in bronchoscopic specimens. Since trimecaine is widely used as local anaesthesia in airways in bronchoscopy, it is necessary to heed these aforementioned effects.

• MeSH
• anestetika lokální aplikace a dávkování   škodlivé účinky   MeSH
• aplikace lokální MeSH
• fukosa metabolismus   MeSH
• glykokonjugáty analýza   metabolismus   MeSH
• histocytochemie metody   MeSH
• intratracheální anestezie škodlivé účinky   MeSH
• králíci MeSH
• lektiny metabolismus   MeSH
• pohárkové buňky chemie   účinky léků   metabolismus   MeSH
• rozvrh dávkování léků MeSH
• trachea MeSH
• trimekain aplikace a dávkování   škodlivé účinky   MeSH
• zvířata MeSH
• Check Tag
• králíci MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• anestetika lokální MeSH
• fukosa MeSH
• glykokonjugáty MeSH
• lektiny MeSH
• trimekain MeSH