The process of acidification of the external medium after addition of glucose and subsequently of KCl to a suspension of yeast cells varies substantially from species to species. After glucose it is most pronounced in Saccharomyces cerevisiae and Schizosaccharomyces pombe but is very much lower in Lodderomyces elongisporus, Dipodascus magnusii and Rhodotorula gracilis. Both the buffering capacity and the varied effects of vanadate, suloctidil and erythrosin B indicate that the acidification is by about one-half due to the activity of plasma membrane H(+)-ATPase and by about one-half to the extrusion of acidic metabolites from cells. This is supported by the finding that a respiratory quotient greater than one (in various strains of S. cerevisiae and in S. pombe) is indicative of a greater buffering capacity and overall acidification of the medium. Taking into account the virtually negligible buffering capacity of the medium in the pH range where the effect of K+ is observed, the effect of K+ is generally of a similar magnitude as that of adding glucose. It is clearly dependent on (anaerobic) production of metabolic energy, quite distinct from the dependence of the H(+)-ATPase-caused acidification.

• MeSH
• biologický transport MeSH
• draslík farmakologie   MeSH
• erythrosin farmakologie   MeSH
• glukosa farmakologie   MeSH
• kationty jednomocné farmakologie   MeSH
• kyseliny metabolismus   MeSH
• protonové ATPasy metabolismus   MeSH
• Saccharomycetales účinky léků   metabolismus   MeSH
• spotřeba kyslíku MeSH
• suloktidil farmakologie   MeSH
• vanadáty farmakologie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• draslík MeSH
• erythrosin MeSH
• glukosa MeSH
• kationty jednomocné MeSH
• kyseliny MeSH
• protonové ATPasy MeSH
• suloktidil MeSH
• vanadáty MeSH

Gas chromatographic separation of metabolites extruded during the medium acidification by resting baker's yeast supplied with glucose is described. Silylation with bis(trimethylsilyl)trifluoroacetamide and trimethylchlorosilane and a direct chromatography on Chromosorb G-AW-DMCS with 2.5% SE-52 (lactic, pyruvic, succinic, glyceric, fumaric, malic), polyols (glycerol, arabinitol) and sugars (glucose). The yeast was found to extrude glycerol, lactic, malic, and succinic acid.

• MeSH
• chromatografie plynová metody   MeSH
• glukosa metabolismus   MeSH
• glycerol metabolismus   MeSH
• laktáty metabolismus   MeSH
• maláty metabolismus   MeSH
• Saccharomyces cerevisiae metabolismus   MeSH
• sukcináty metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• glukosa MeSH
• glycerol MeSH
• laktáty MeSH
• maláty MeSH
• sukcináty MeSH