Gas chromatographic separation of metabolites extruded during the medium acidification by resting baker's yeast supplied with glucose is described. Silylation with bis(trimethylsilyl)trifluoroacetamide and trimethylchlorosilane and a direct chromatography on Chromosorb G-AW-DMCS with 2.5% SE-52 (lactic, pyruvic, succinic, glyceric, fumaric, malic), polyols (glycerol, arabinitol) and sugars (glucose). The yeast was found to extrude glycerol, lactic, malic, and succinic acid.

• MeSH
• Chromatography, Gas methods   MeSH
• Glucose metabolism   MeSH
• Glycerol metabolism   MeSH
• Lactates metabolism   MeSH
• Malates metabolism   MeSH
• Saccharomyces cerevisiae metabolism   MeSH
• Succinates metabolism   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Glucose MeSH
• Glycerol MeSH
• Lactates MeSH
• Malates MeSH
• Succinates MeSH