Oligosaccharides produced by submerged cultures of C. africana and C. sorghi were isolated by semipreparative HPLC. Structure of 6-O-beta-D-fructofuranosyl-D-glucopyranose (blastose), 1,6-bis-O-(beta-D-fructofuranosyl)-alpha-D-glucopyranoside (neokestose) and two sugar alcohols, 1-O-beta-D-fructofuranosyl-D-mannitol (fructosylmannitol) and 1,6-bis-O-(beta-D-fructofuranosyl)-D-mannitol (bisfructosylmannitol) was determined by NMR spectrometry. MALDI TOF MS analysis revealed molecular ions [M+Na]+ that indicate the presence of other tetra- and pentasaccharides (m/z = 689.4 and 851.5, respectively) and corresponding sugar alcohol (m/z = 691.4). Rapid conversion of sucrose into series of oligosaccharides and corresponding sugar alcohols was observed in all tested strains.

• MeSH
• Claviceps growth & development   isolation & purification   metabolism   pathogenicity   MeSH
• Sugar Alcohols chemistry   metabolism   MeSH
• Fermentation MeSH
• Molecular Structure MeSH
• Plant Diseases microbiology   MeSH
• Nuclear Magnetic Resonance, Biomolecular MeSH
• Oligosaccharides biosynthesis   chemistry   isolation & purification   MeSH
• Sorghum microbiology   MeSH
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization MeSH
• Chromatography, High Pressure Liquid MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Sugar Alcohols MeSH
• Oligosaccharides MeSH

A total of 307 new compounds, natural, semisynthetic or synthetic, were isolated at the Institute of microbiology during the last twelve years. Due to the development of separation (chromatographic) methods and of analytical methods used to determine the chemical structure of these compounds, i.e. NMR, MS and X-ray diffraction, many new metabolites could be described.

• MeSH
• Biological Factors chemistry   isolation & purification   MeSH
• Peptides, Cyclic chemistry   isolation & purification   MeSH
• Enzymes chemistry   isolation & purification   MeSH
• Molecular Sequence Data MeSH
• Ergot Alkaloids chemistry   isolation & purification   MeSH
• Carbohydrate Sequence MeSH
• Carbohydrates chemistry   isolation & purification   MeSH
• Amino Acid Sequence MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Review MeSH
• Names of Substances
• Biological Factors MeSH
• Peptides, Cyclic MeSH
• Enzymes MeSH
• Ergot Alkaloids MeSH
• Carbohydrates MeSH

Natural sources, i.e. fungal strains and species producing ergot alkaloids (EA), are surveyed together with the chemical structures of EA and a list of new natural EA discovered in the last three decades. Other topics include new efficient chromatographic methods (HPLC) for the separation and isolation of new natural EA and also immunological methods of EA detection.

• MeSH
• Fungi chemistry   MeSH
• Immunoassay methods   MeSH
• Humans MeSH
• Molecular Structure MeSH
• Ergot Alkaloids chemistry   isolation & purification   pharmacology   MeSH
• Chromatography, High Pressure Liquid methods   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Review MeSH
• Names of Substances
• Ergot Alkaloids MeSH