In this report, we describe 12 subpopulations of porcine gammadelta thymocytes based on their expression of CD1, CD2, CD4, CD8- isoforms and CD45RC. Our data suggest that gammadelta thymocytes can be divided into two major families: (a) one large family of CD4-gammadelta thymocytes that could be further subdivided according to the CD2/CD8alphaalpha phenotype and (b) a small family of CD4+ gammadelta thymocytes bearing CD8alphabeta and possessing certain unusual features in comparison with other gammadelta thymocytes. Maturation of gammadelta thymocytes within the CD4- family begins with proliferation of the CD2+ CD8- CD1+ CD45RC- gammadelta common precursor. This developmental stage is followed by diversification into the CD2+ CD8alphaalpha+, CD2+ CD8- and CD2- CD8- subsets. Their further maturation is accompanied by a loss of expression of CD1 and by increased expression of CD45RC. Therefore, individual subsets develop from CD1+ CD45RC- through CD1- CD45RC- into CD1- CD45RC+ cells. On the other hand, gammadelta thymocytes within the CD4+ family bear exclusively CD8alphabeta, always express CD1, but may coexpress CD45RC. These cells have no counterpart in the periphery. Our observations suggest that all peripheral CD8+ gammadelta T cells express CD8alphaalpha and that two subsets of these cells differing in major histocompatibility complex II expression, occur. We propose that one subset acquires CD8alphaalpha in the thymus while the second acquires CD8alphaalpha as a result of stimulation in the periphery.

• MeSH
• antigeny CD1 imunologie   MeSH
• antigeny CD2 imunologie   MeSH
• antigeny CD45 imunologie   MeSH
• antigeny CD8 imunologie   MeSH
• buněčná diferenciace imunologie   MeSH
• buněčné dělení imunologie   MeSH
• CD antigeny imunologie   MeSH
• CD4-pozitivní T-lymfocyty imunologie   MeSH
• CD8-pozitivní T-lymfocyty imunologie   MeSH
• imunofenotypizace metody   MeSH
• kultivované buňky MeSH
• MHC antigeny II. třídy imunologie   MeSH
• modely u zvířat MeSH
• prasata MeSH
• receptory antigenů T-buněk gama-delta imunologie   MeSH
• slezina cytologie   imunologie   MeSH
• T-lymfocyty - podskupiny imunologie   MeSH
• thymus cytologie   embryologie   imunologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny CD1 MeSH
• antigeny CD2 MeSH
• antigeny CD45 MeSH
• antigeny CD8 MeSH
• CD antigeny MeSH
• MHC antigeny II. třídy MeSH
• receptory antigenů T-buněk gama-delta MeSH