OBJECTIVE: To analyze relations among acute phase reactants in a group of 40 women operated for uterine myom by laparoscopy and open surgery. DESIGN: Prospective study. METHODS: Plasma concentrations of C-reactive protein (CRP), serum amyloid A (SAA) and interleukin 6 (IL-6) were measured together with leukocytes in blood before operation, 24 and 72 hours post operation, respectively. RESULTS: Leukocytes and IL-6 displayed minimal response and decreased quickly after operation to preoperative levels. Concentrations of CRP and SAA remained increased after operation. There were no relationships between leukocytes and acute phase reactants. Normal leukocytes 72 hours post operation were found in 1/3 of women with increased at least one acute phase reactants and in 1/4 of women with increased at least two markers. Typ of surgery, surgical stress and length of surgery were related to the concentration of CRP, IL-6 and SAA. CONCLUSION: Changes in SAA 24 hours after operation are similar to CRP and IL-6. Surgical stress, length of operation and possible risk 72 hours after operation are best predicted by CRP and SAA (at that time IL-6 and leukocytes are practically normal). Maximal increase was found for SAA concentrations. Thus SAA seems to be suitable marker of early postoperative complications.

• MeSH
• biologické markery krev   MeSH
• C-reaktivní protein analýza   MeSH
• dospělí MeSH
• fyziologický stres krev   diagnóza   etiologie   MeSH
• gynekologické chirurgické výkony škodlivé účinky   MeSH
• interleukin-6 krev   MeSH
• leiomyom chirurgie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• nádory dělohy chirurgie   MeSH
• počet leukocytů MeSH
• pooperační komplikace krev   diagnóza   MeSH
• pooperační období MeSH
• reakce akutní fáze krev   diagnóza   etiologie   MeSH
• sérový amyloid A analýza   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• biologické markery MeSH
• C-reaktivní protein MeSH
• interleukin-6 MeSH
• sérový amyloid A MeSH

The acute inflammatory response associated with thermal injury was examined in rats. The appearance of mediators of inflammation in the systemic circulation, including cytokines interleukin-1 (IL-1), tumor necrosis factor (TNF) and interleukin-6 (IL-6) and acute phase proteins were assessed during initial 72 h following thermal injury. Increased levels of activity were noted for all three cytokines, but with a different time-course. While serum IL-1 activity was elevated throughout the 3-day period of observation, the levels of serum TNF activity were enhanced after 12 h and on days 1 and 3 following scalding injury. The values of IL-6 were already increased one hour after thermal injury and increased progressively up to day 1 following scalding. Alpha2-macroglobulin and haptoglobin levels were increased 12 h after thermal injury, rising further on days 1 and 3. Positive correlation was found between the time-course of increased serum IL-6 activity and alpha2-macroglobulin, as well as between TNF and haptoglobin in the serum.

• MeSH
• alfa-makroglobuliny imunologie   metabolismus   MeSH
• fyziologický stres imunologie   MeSH
• haptoglobiny metabolismus   MeSH
• hematokrit MeSH
• inbrední kmeny potkanů MeSH
• interleukin-1 krev   imunologie   MeSH
• interleukin-6 krev   imunologie   MeSH
• krysa rodu Rattus MeSH
• popálení imunologie   MeSH
• reakce akutní fáze imunologie   MeSH
• regresní analýza MeSH
• sérový albumin MeSH
• tělesná teplota MeSH
• TNF-alfa imunologie   metabolismus   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• alfa-makroglobuliny MeSH
• haptoglobiny MeSH
• interleukin-1 MeSH
• interleukin-6 MeSH
• sérový albumin MeSH
• TNF-alfa MeSH

UNLABELLED: Leptin is a hormone and acute phase reactant with multiple effects during both rest and inflammatory period. Effects of leptin in acute phase response cover a modulation of hypothalamo-pituitoadrenal (HPA) axis, too. In our study we evaluate the dynamics of leptin and glucocorticoid acute changes in model situations of infectious and non-infectious inflammatory reaction. PATIENTS AND METHODS: This study was realized on a group of patients 1--after operation of carcinoma coli (16 persons), 2--after laparoscopic non-adjustable gastric banding (13 persons), and patients 3--with development of postoperative intraabdominal sepsis (22 persons). Plasma levels of leptin, ACTH, cortisol, TNF-alpha, IL-6, C reactive protein (CRP), and alpha1-antitrypsin (AAT) were measured from -7 to +3 day related to surgery or 2nd day after a day when signs of sepsis were occurred. RESULTS: All tested parameters responded to surgical trauma with elevation 12 h after operation except leptin that elevated +24 h after surgery. Maximal levels of measured parameters were found 12 h after surgery (for cortisol), 24 h after surgery (for leptin, TNF-alpha, IL-6), or 36 h after operation (for CRP and AAT). Maximal levels of cortisol after a resection of coli or maximal levels of leptin after laparoscopic gastric banding fall to septic range. Other parameters in uncomplicated postoperative period significantly differed from septic levels on p < 0.01. There wasn't found statistically significant correlation between leptin and cortisol or between leptin and ACTH in all groups. DISCUSSION AND CONCLUSION: The elevation of leptin in postoperative period and during sepsis reflects a different regulation of this protein in rest period and during systemic inflammatory response. The inhibitory effect of leptin to HPA axis during stress response was documented in vitro, however, its physiological importance is not clear, yet. Our study didn't prove a statistically significant relation between plasma cortisol and leptin. The different dynamics of leptin and cortisol after surgical trauma shows that both factors have own specific regulation.

• MeSH
• adrenokortikotropní hormon krev   MeSH
• alfa-1-antitrypsin analýza   MeSH
• C-reaktivní protein analýza   MeSH
• chirurgie operační škodlivé účinky   MeSH
• dospělí MeSH
• fyziologický stres krev   etiologie   MeSH
• gastroplastika MeSH
• hydrokortison krev   MeSH
• kolorektální nádory chirurgie   MeSH
• leptin krev   MeSH
• lidé středního věku MeSH
• lidé MeSH
• reakce akutní fáze krev   etiologie   MeSH
• senioři MeSH
• sepse krev   MeSH
• TNF-alfa analýza   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• adrenokortikotropní hormon MeSH
• alfa-1-antitrypsin MeSH
• C-reaktivní protein MeSH
• hydrokortison MeSH
• leptin MeSH
• TNF-alfa MeSH

The oxidative mechanisms of injury-induced damage of neurons within the spinal cord are not very well understood. We used a model of T8-T9 spinal cord injury (SCI) in the rat to induce neuronal degeneration. In this spinal cord injury model, unilateral avulsion of the spinal cord causes oxidative stress of neurons. We tested the hypothesis that apurinic/apyrimidinic endonuclease (or redox effector factor-1, APE/Ref-1) regulates this neuronal oxidation mechanism in the spinal cord region caudal to the lesion, and that DNA damage is an early upstream signal. The embryonic neural stem cell therapy significantly decreased DNA-damage levels in both study groups - acutely (followed up to 7 days after SCI), and chronically (followed up to 28 days after SCI) injured animals. Meanwhile, mRNA levels of APE/Ref-1 significantly increased after embryonic neural stem cell therapy in acutely and chronically injured animals when compared to acute and chronic sham groups. Our data has demonstrated that an increase of APE/Ref-1 mRNA levels in the caudal region of spinal cord strongly correlated with DNA damage after traumatic spinal cord injury. We suggest that DNA damage can be observed both in lesional and caudal regions of the acutely and chronically injured groups, but DNA damage is reduced with embryonic neural stem cell therapy.

• MeSH
• akutní nemoc MeSH
• cauda equina enzymologie   patologie   chirurgie   MeSH
• chronická nemoc MeSH
• degenerace nervu enzymologie   genetika   patologie   chirurgie   MeSH
• DNA-lyasa (apurinová nebo apyrimidinová) genetika   metabolismus   MeSH
• embryonální kmenové buňky enzymologie   patologie   transplantace   MeSH
• kometový test MeSH
• krysa rodu Rattus MeSH
• kultivované buňky MeSH
• lokomoce MeSH
• messenger RNA metabolismus   MeSH
• modely nemocí na zvířatech MeSH
• neurony enzymologie   patologie   transplantace   MeSH
• oxidační stres MeSH
• polymerázová řetězová reakce s reverzní transkripcí MeSH
• poranění míchy enzymologie   genetika   patologie   chirurgie   MeSH
• poškození DNA * MeSH
• potkani Sprague-Dawley MeSH
• upregulace MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• Apex1 protein, rat MeSH Prohlížeč
• DNA-lyasa (apurinová nebo apyrimidinová) MeSH
• messenger RNA MeSH

The reports of analgesic effects of benzodiazepines are inconsistent. There is evidence of a hyperalgesic effect induced by activation of supraspinal GABAA receptors and an antinociceptive effect induced by activation of receptors located in the spinal cord (dorsal horns). The aim of the study was to discover whether the systemic administration of a benzodiazepine agent alprazolam increases the systemic analgesic efficacy of non-opioid analgesic ibuprofen. Experimental studies combining these agents have not yet been published. We used three experimental methods - writhing test (with acetic acid), tail-flick test and plantar test to assess analgesic action. The drugs were administered orally. Augmentation of the analgesic effect of ibuprofen by alprazolam was proved for the writhing test at a dose of 30 mg/kg of ibuprofen and alprazolam 1 mg/kg. The reaction time of the combination was significantly prolonged in comparison with ibuprofen alone. The results of the tail-flick test and plantar test were negative. The effect of ibuprofen was not enhanced by alprazolam in tests of acute thermal pain. Our results have demonstrated that the analgesic action of ibuprofen is only weakly enhanced by alprazolam.

• MeSH
• akutní nemoc MeSH
• alprazolam farmakologie   MeSH
• aplikace orální MeSH
• bolest chemicky indukované   farmakoterapie   MeSH
• GABA modulátory farmakologie   MeSH
• ibuprofen farmakologie   MeSH
• inbrední kmeny myší MeSH
• kyselina octová MeSH
• modely nemocí na zvířatech MeSH
• myši MeSH
• neopioidní analgetika farmakologie   MeSH
• nociceptory účinky léků   MeSH
• receptory GABA-A fyziologie   MeSH
• synergismus léků MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• alprazolam MeSH
• GABA modulátory MeSH
• ibuprofen MeSH
• kyselina octová MeSH
• neopioidní analgetika MeSH
• receptory GABA-A MeSH

OBJECTIVES: The aim of the study was to investigate the effects of subchronic exposure of zebrafish to ibuprofen, using selected oxidative stress parameters as a target. DESIGN: Toxicity tests were performed on Danio rerio according to OECD No. 203 and No. 215. In the growth test, fish were exposed to subletal concentrations of ibuprofen (0.0001, 0.05, 1, 8, and 25 mg.L-1) for 28 days. For the assessment of free radical defense in fish, the catalytic activities of glutathione reductase (GR), glutathione S-transferase (GST), glutathione peroxidase (GPx), and catalase (CAT), as well as the concentration of malondialdehyde (MDA) were measured. RESULTS: Ibuprofen did not affect the activity of glutathione reductase and catalase. A significant (p<0.01) increase in the activity of glutathione peroxidase was found, which was proved dose-dependent (10.58 nmol NADPH per min per mg protein in the control and 20.53, 26.36, 26.89, and 45.87 nmol NADPH per min per mg protein in the ibuprofen concentrations of 0.5, 1, 8, and 25 mg.L-1. An increased (p<0.05) activity of glutathione S-transferase in the highest concentration was found compared to control. Malondialdehyde levels were found significantly (p<0.01) decreased from control in the concentrations of 0.0001 and 8 mg.L-1, but no dose-dependence was found. CONCLUSION: The results suggest that ibuprofen causes the increase in the activity of some antioxidative and biotransformation enzymes in zebrafish (GPx and GST). We also found a significant decrease in lipid peroxidation in the concentrations of 0.0001 and 8 mg.L-1 compared to control.

• MeSH
• antiflogistika nesteroidní toxicita   MeSH
• biologické markery metabolismus   MeSH
• dánio pruhované * MeSH
• glutathionperoxidasa metabolismus   MeSH
• glutathionreduktasa metabolismus   MeSH
• glutathiontransferasa metabolismus   MeSH
• ibuprofen toxicita   MeSH
• katalasa metabolismus   MeSH
• oxidační stres účinky léků   MeSH
• peroxidace lipidů účinky léků   MeSH
• testy akutní toxicity MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antiflogistika nesteroidní MeSH
• biologické markery MeSH
• glutathionperoxidasa MeSH
• glutathionreduktasa MeSH
• glutathiontransferasa MeSH
• ibuprofen MeSH
• katalasa MeSH