PIWI-interacting RNAs (piRNAs) support the germline by suppressing retrotransposons. Studies of the pathway in mice have strongly shaped the view that mammalian piRNAs are essential for male but not for female fertility. Here, we report that the role of the piRNA pathway substantially differs in golden hamsters (Mesocricetus auratus), the piRNA pathway setup of which more closely resembles that of other mammals, including humans. The loss of the Mov10l1 RNA helicase-an essential piRNA biogenesis factor-leads to striking phenotypes in both sexes. In contrast to mice, female Mov10l1-/- hamsters are sterile because their oocytes do not sustain zygotic development. Furthermore, Mov10l1-/- male hamsters have impaired establishment of spermatogonia accompanied by transcriptome dysregulation and an expression surge of a young retrotransposon subfamily. Our results show that the mammalian piRNA pathway has essential roles in both sexes and its adaptive nature allows it to manage emerging genomic threats and acquire new critical roles in the germline.
- MeSH
- křečci praví MeSH
- křeček rodu Mesocricetus metabolismus MeSH
- malá interferující RNA genetika MeSH
- oocyty metabolismus patologie MeSH
- retroelementy fyziologie MeSH
- RNA-helikasy genetika MeSH
- spermatogeneze genetika fyziologie MeSH
- spermatogonie metabolismus patologie MeSH
- testis metabolismus MeSH
- umlčování genů fyziologie MeSH
- zvířata MeSH
- Check Tag
- křečci praví MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- malá interferující RNA MeSH
- retroelementy MeSH
- RNA-helikasy MeSH
The brown adipose tissue of cold adapted hamsters has an approximately 35% higher phospholipid content than that of the controls. The basal turnover rate of phosphatidylinositol is higher in cold-adapted animals than in the controls, whereas 32P incorporation into phosphatidylcholine, phosphatidylethanolamine and diphosphatidylglycerol is not changed. Administration of norepinephrine increases the incorporation of 32P into both control and cold-adapted animals by 200% and 220% respectively. The 32P incorporation into other phospholipid fractions is not modified.
- MeSH
- fosfatidylcholiny metabolismus MeSH
- fosfatidylethanolaminy metabolismus MeSH
- fosfatidylinositoly metabolismus MeSH
- fosfolipidy metabolismus MeSH
- fyziologická adaptace MeSH
- hnědá tuková tkáň metabolismus MeSH
- kardiolipiny metabolismus MeSH
- křečci praví MeSH
- křeček rodu Mesocricetus metabolismus MeSH
- nízká teplota * MeSH
- noradrenalin farmakologie MeSH
- zvířata MeSH
- Check Tag
- křečci praví MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- fosfatidylcholiny MeSH
- fosfatidylethanolaminy MeSH
- fosfatidylinositoly MeSH
- fosfolipidy MeSH
- kardiolipiny MeSH
- noradrenalin MeSH
Biliary excretion of arsenic and its distribution in the organism were studied in golden hamsters after administration of Na3(74)AsO3 and Na2H74AsO4 solutions. Significant differences between the two valency forms of arsenic were found in the biliary excretion rate as well as in the cumulative biliary excretion of 74As. The arsenic biliary excretion after treatment with 74AS [III] [4.98 /+- 7.1, or 0.6 [0.2 - 1.1] percents of the administered dose] than that after 74As [V] application. On the other hand, arsenic excretion in the urine and stool were higher after the administration of pentavalent arsenic. The contents of 74As in the liver, kidneys, blood plasma and GIT wall were higher after the application of 74As [III]. It has been again confirmed tha compared to rats the hamster [similarly as the other laboratory animals] exhibits a significantly lower concentration of 74As in red blood cells [differences ranged by 2 orders of magnitude].
- MeSH
- arsen metabolismus MeSH
- časové faktory MeSH
- druhová specificita MeSH
- křečci praví metabolismus MeSH
- křeček rodu Mesocricetus metabolismus MeSH
- radionuklidy * MeSH
- tkáňová distribuce MeSH
- žluč metabolismus MeSH
- zvířata MeSH
- Check Tag
- křečci praví metabolismus MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- arsen MeSH
- radionuklidy * MeSH
1. Mitochondrial membrane of brown adipose tissue compared to that of liver possesses a very high activity of oxidative enzymes but a low activity of ATPase. 2. The polypeptide composition of the mitochondrial membranes proves that the above differences in enzyme activities are due to increased content of oxidative enzymes and decreased content of ATPase in brown adipose tissue. 3. The inhibition of ATPase of brown adipose tissue mitochondria by aurovertin, oligomycin and DCCD indicates modified proportions between the components of the ATPase complex. 4. The organization of brown adipose tissue mitochondrial membrane in relation to its thermogenic function is discussed.
