Spatial light modulators have become an essential tool for advanced microscopy, enabling breakthroughs in 3D, phase, and super-resolution imaging. However, continuous spatial-light modulation that is capable of capturing sub-millisecond microscopic motion without diffraction artifacts and polarization dependence is challenging. Here we present a photothermal spatial light modulator (PT-SLM) enabling fast phase imaging for nanoscopic 3D reconstruction. The PT-SLM can generate a step-like wavefront change, free of diffraction artifacts, with a high transmittance and a modulation efficiency independent of light polarization. We achieve a phase-shift > π and a response time as short as 70 µs with a theoretical limit in the sub microsecond range. We used the PT-SLM to perform quantitative phase imaging of sub-diffractional species to decipher the 3D nanoscopic displacement of microtubules and study the trajectory of a diffusive microtubule-associated protein, providing insights into the mechanism of protein navigation through a complex microtubule network.

• MeSH
• Time Factors MeSH
• Microscopy, Interference methods   statistics & numerical data   MeSH
• Metal Nanoparticles ultrastructure   MeSH
• Humans MeSH
• Microscopy, Atomic Force MeSH
• Microscopy, Phase-Contrast methods   statistics & numerical data   MeSH
• Microtubules metabolism   ultrastructure   MeSH
• Nanotechnology MeSH
• Nanotubes ultrastructure   MeSH
• Optical Phenomena MeSH
• Computer Simulation MeSH
• Microtubule-Associated Proteins metabolism   MeSH
• Cell Cycle Proteins metabolism   MeSH
• Schizosaccharomyces pombe Proteins metabolism   MeSH
• Light MeSH
• Tubulin metabolism   MeSH
• Gold MeSH
• Imaging, Three-Dimensional methods   statistics & numerical data   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Ase1 protein, S pombe MeSH Browser
• PRC1 protein, human MeSH Browser
• Microtubule-Associated Proteins MeSH
• Cell Cycle Proteins MeSH
• Schizosaccharomyces pombe Proteins MeSH
• Tubulin MeSH
• Gold MeSH

Transmitted-light coherence-controlled holographic microscope (CCHM) based on an off-axis achromatic interferometer allows us to use light sources of arbitrary degree of temporal and spatial coherence. Besides the conventional DHM modes such as quantitative phase contrast imaging and numerical 3D holographic reconstruction it provides high quality (speckle-free) imaging, improved lateral resolution and optical sectioning by coherence gating. Optical setup parameters and their limits for a technical realization are derived and described in detail. To demonstrate the optical sectioning property of the microscope a model sample uncovered and then covered with a diffuser was observed using a low-coherence light source.

• MeSH
• Algorithms MeSH
• Equipment Design instrumentation   MeSH
• Holography methods   MeSH
• Microscopy, Interference methods   MeSH
• Interferometry methods   MeSH
• Image Interpretation, Computer-Assisted instrumentation   methods   MeSH
• Microscopy, Confocal methods   MeSH
• Lasers MeSH
• Humans MeSH
• Microscopy, Phase-Contrast methods   MeSH
• Nephelometry and Turbidimetry MeSH
• Optics and Photonics * MeSH
• Image Processing, Computer-Assisted MeSH
• Imaging, Three-Dimensional methods   MeSH
• Animals MeSH
• Check Tag
• Humans MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, U.S. Gov't, Non-P.H.S. MeSH

A new myxosporean species, Trilosporoides platessae gen. et sp. n. (Multivalvulida), is described from the gallbladder of the plaice Pleuronectes platessa L. (Pleuronectidae) from Denmark. The myxospore of T. platessae is conical in side view, with a 24 microm long, pointed posterior projection. In apical view, the myxospore (diameter 9.4 microm) is round, trilobed and with three spherical polar capsules arranged peripherally, equidistant and opening peripherally through protruding tips. The polar capsules are of different sizes, one often larger than the others (diameter 3.3 microm vs. 2.5 microm). Apart from the long posterior projection, the myxospore of T. platessae differs from those of the three known species of Trilospora Noble, 1959 and from all genera within the order Multivalvulida Shulman, 1959 in the arrangement of the polar capsules. Trilosporoides platessae may temporarily be placed in the vicinity of the Trilosporidae.

• MeSH
• Eukaryota classification   cytology   isolation & purification   parasitology   MeSH
• Microscopy, Interference methods   MeSH
• Fish Diseases parasitology   MeSH
• Flounder parasitology   MeSH
• Protozoan Infections, Animal parasitology   MeSH
• Body Size MeSH
• Gallbladder parasitology   MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Journal Article MeSH
• Comparative Study MeSH
• Geographicals
• Denmark MeSH