The clinical consequences of toxoplasmosis are greatly dependent on the Toxoplasma gondii strain causing the infection. To better understand its epidemiology and design appropriate control strategies, it is important to determine the strain present in infected animals. Serotyping methods are based on the detection of antibodies that react against segments of antigenic proteins presenting strain-specific polymorphic variations, offering a cost-effective, sensitive, and non-invasive alternative to genotyping techniques. Herein, we evaluated the applicability of a panel of peptides previously characterized in mice and humans to serotype sheep and pigs. To this end, we used 51 serum samples from experimentally infected ewes (32 type II and 19 type III), 20 sheep samples from naturally infected sheep where the causative strain was genotyped (18 type II and 2 type III), and 40 serum samples from experimentally infected pigs (22 type II and 18 type III). Our ELISA test results showed that a combination of GRA peptide homologous pairs can discriminate infections caused by type II and III strains of T. gondii in sheep and pigs. Namely, the GRA3-I/III-43 vs. GRA3-II-43, GRA6-I/III-213 vs. GRA6-II-214 and GRA6-III-44 vs. GRA6-II-44 ratios showed a statistically significant predominance of the respective strain-type peptide in sheep, while in pigs, in addition to these three peptide pairs, GRA7-II-224 vs. GRA7-III-224 also showed promising results. Notably, the GRA6-44 pair, which was previously deemed inefficient in mice and humans, showed a high prediction capacity, especially in sheep. By contrast, GRA5-38 peptides failed to correctly predict the strain type in most sheep and pig samples, underpinning the notion that individual standardization is needed for each animal species. Finally, we recommend analyzing for each animal at least 2 samples taken at different time points to confirm the obtained results.

• Klíčová slova
• GRA, Toxoplasma gondii, peptide, pig, serotyping, sheep,
• MeSH
• antigeny protozoální * genetika   imunologie   MeSH
• ELISA metody   MeSH
• genotyp MeSH
• nemoci ovcí * parazitologie   diagnóza   MeSH
• nemoci prasat * parazitologie   diagnóza   MeSH
• ovce MeSH
• peptidy imunologie   MeSH
• prasata MeSH
• protilátky protozoální krev   MeSH
• sérotypizace * metody   MeSH
• toxoplazmóza zvířat * diagnóza   parazitologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny protozoální * MeSH
• GRA6 protein, Toxoplasma gondii MeSH Prohlížeč
• GRA7 protein, Toxoplasma gondii MeSH Prohlížeč
• peptidy MeSH
• protilátky protozoální MeSH