• Klíčová slova
• CATALASE *, CEREBROSPINAL FLUID *,
• MeSH
• katalasa * MeSH
• lidé MeSH
• mozkomíšní mok * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• katalasa * MeSH

• Klíčová slova
• CATALASE/cerebrospinal fluid *,
• MeSH
• katalasa mozkomíšní mok   MeSH
• lidé MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• katalasa MeSH

• MeSH
• katalasa metabolismus   MeSH
• lidé MeSH
• manometrie MeSH
• mozkomíšní mok enzymologie   MeSH
• techniky in vitro MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• katalasa MeSH

• Klíčová slova
• CATALASE *, CEREBROSPINAL FLUID *, CEREBROSPINAL FLUID PROTEINS *, CEREBROVASCULAR DISORDERS *, DIAGNOSIS, DIFFERENTIAL *, ERYTHROCYTES *,
• MeSH
• cerebrovaskulární poruchy * MeSH
• diferenciální diagnóza * MeSH
• erytrocyty * MeSH
• katalasa * MeSH
• lidé MeSH
• mozkomíšní mok * MeSH
• proteiny v mozkomíšním moku * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• katalasa * MeSH
• proteiny v mozkomíšním moku * MeSH

The species of Comamonas testosteroni is the most common human pathogen of the genus, which can be associated with acute appendicitis, infections of the bloodstream, the peritoneal cavity, cerebrospinal fluid, inflammatory bowel disease, and in general, bacteremia. According to the literature, Comamonas testosteroni has destructive activity to a wide range of toxic chemical compounds, including chlorobenzenes. The specified strains were isolated from the soil of the organochlorine waste landfill, where hexachlorobenzene (HCB) was predominant. These strains were expected to be capable of degrading HCB. Microbiological (bacterial enrichment and cultivating, bacterial biomass obtaining), molecular biology, biochemical (enzymatic activities, malondialdehyde measuring, peroxidation lipid products measuring), and statistical methods were carried out in this research. The reaction of both strains (UCM B-400 and UCM B-401) to the hexachlorobenzene presence differed in the content of diene and triene conjugates and malondialdehyde, as well as different catalase and peroxidase activity levels. In terms of primary peroxidation products, diene conjugates were lower, except conditions with 20 mg/L HCB, where these were higher up to two times, than the pure control. Malondialdehyde in strain B-400 cells decreased up to five times, in B-401, but increased up to two times, compared to the pure control. Schiff bases in strain B-400 cells were 2-3 times lower than the pure control. However, in B-401 cells Schiff bases under higher HCB dose were in the same level with the pure control. Catalase activity was 1.5 times higher in all experimental variants, compared to the pure control (in the strain B-401 cells), but in the B-400 strain, cells were 2 times lower, compared to the pure control. The response of the two strains to hexachlorobenzene was similar only in peroxidase activity terms, which was slightly higher compared to the pure control. The physiological response of Comamonas testosteroni strains to hexachlorobenzene has a typical strain reaction. The physiological response level of these strains to hexachlorobenzene confirms its tolerance, and indirectly, the ability to destroy the specified toxic compound.

• Klíčová slova
• Schiff bases, catalase, diene and triene conjugates, hexachlorobenzene, malondialdehyde, peroxidase,
• MeSH
• antioxidancia MeSH
• chlorbenzeny MeSH
• Comamonas testosteroni * MeSH
• hexachlorbenzen * MeSH
• katalasa MeSH
• lidé MeSH
• lipidy MeSH
• malondialdehyd MeSH
• peroxidace lipidů MeSH
• půda MeSH
• Schiffovy báze MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antioxidancia MeSH
• chlorbenzeny MeSH
• hexachlorbenzen * MeSH
• katalasa MeSH
• lipidy MeSH
• malondialdehyd MeSH
• půda MeSH
• Schiffovy báze MeSH

Starting from 1970, the notification of N. meningitidis cases in Poland was compulsory and separated from other cases of meningitis purulenta. Based on the experience of European Monitoring Group on Meningococci, the active surveillance of meningococcal meningitis in Poland was initiated in April 1995. It was the first time that such study was conducted to recognise the actual situation of meningococcal meningitis infections in our country. Ninety seven N. meningitidis strains were isolated (31 in 1995 and 66 in 1996) from cerebrospinal fluid (CSF) of meningitis patients hospitalized in 54 hospitals located in 33 out of 49 provinces of Poland. Most patients were below 2 years of age and 43% belonged to infant group. Meningococcal strains were phenotypically characterized as follow: identification of N. meningitidis was performed by Gram staining, oxidase and catalase tests as well as latex or diagnostic sera agglutination assays. Meningococcal serotypes and subtypes were determined by whole-cell ELISA with monoclonal antibodies. The predominant meningococcal serogroup during 1995 and 1996 was B (80% of all isolates tested), the serogroup C (12.6%) and W-135 (3.5%). Only two non-groupable and two serogroup A strains were isolated in Poland. Active surveillance allowed to determine B:22:P1.14 to be the most prevalent N. meningitidis phenotype in Poland. Two isolates of N. meningitidis phenotype C:2a:P1.2,5, which caused emergency situation in Czech Republic since 1993, were isolated from CSF of patients in October 1996 in southern Poland. All strains were susceptible to cefotaxime, chloramphenicol, ciprofloxacin, rifampin and tetracycline; some strains were resistant to sulphonamides (60.6% - MIC = 32 mg/l and 14.8% - MIC = 128 mg/l). Only one of the tested strains in two years surveillance study in Poland was resistant to penicillin (MIC = 2 mg/l).

• MeSH
• dítě MeSH
• dospělí MeSH
• ELISA MeSH
• fenotyp MeSH
• kojenec MeSH
• latex fixační testy MeSH
• lidé středního věku MeSH
• lidé MeSH
• meningokoková meningitida mozkomíšní mok   epidemiologie   MeSH
• mikrobiální testy citlivosti MeSH
• mladiství MeSH
• Neisseria meningitidis účinky léků   izolace a purifikace   MeSH
• novorozenec MeSH
• předškolní dítě MeSH
• prevalence MeSH
• senioři MeSH
• surveillance populace MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• kojenec MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• novorozenec MeSH
• předškolní dítě MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Polsko epidemiologie   MeSH