Using quantitative data available from the literature on murine hematopoiesis, the functional reserve of multipotential stem cells was calculated by comparing daily blood cell production with the potential of clonogenic spleen colony-forming cells (CFU-S) to generate blood cells. The potential of the day-8 CFU-S (CFU-S-8) population is estimated to be from 4000 to 37,000 times greater than needed in steady-state hematopoiesis. The CFU-S population may thus serve to provide a functional reserve to supply large numbers of peripheral blood cells via committed lineage-specific cells within days, whenever needed.

• MeSH
• buněčné dělení MeSH
• hematopoetické kmenové buňky cytologie   MeSH
• hematopoéza MeSH
• lidé MeSH
• myši MeSH
• slezina cytologie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH

We examined the effects of 5 soluble derivatives of yeast glucan on the formation of exogenous (CFU-S) and endogenous (E-CFU) colony-forming units in the spleens of sublethally irradiated (60Co, 6.5-7.0 Gy) mice of two inbred strains. For the estimation of CFU-S, glucans were administered intravenously either to donors or recipients of spleen cells 24 h prior to irradiation or removal of the spleen. The number of CFU-S was increased when both the donors and recipients were treated with glucan; the highest increase was obtained with glucans S, P and K. All glucan preparations increased significantly also the number of E-CFU even when administered 90 min after irradiation. There exist differences in the response to the stimulatory effect of glucans among individual mouse strains. Thus, for example, the stimulatory effect of glucan KM on the E-CFU number was significantly more pronounced in strain A/Ph than in strain C57Bl/6.

• MeSH
• analýza kolonii tvořících jednotek MeSH
• glukany farmakologie   MeSH
• hematopoetické kmenové buňky účinky léků   MeSH
• myši inbrední A MeSH
• myši inbrední C57BL MeSH
• myši MeSH
• Saccharomyces cerevisiae * MeSH
• slezina cytologie   účinky léků   účinky záření   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• glukany MeSH

A linear model for the errors of the 'spleen colony' assay for haemopoietic stem cells has been derived. The components emerging from the model are interpreted and practical recommendations given for interpreting measurements made with this assay. The model permits correction for the effect of overlapping colonies and gives average errors for single measurements of the number of CFU-s. More reliable and more precise information can be obtained using this model. The spleen colony technique detects a population of immature precursor cells designated as CFU-s (Till & McCulloch, 1961). The relative error of measurement is often large when compared with the changes in the phenomena studied. Consequently a better knowledge of the errors of this technique is highly desirable. This paper should be regarded as an extension of the previous analysis of Till (1972). The theory for the errors of the spleen colony technique was applied to 905 determinations of the CFU-s numbers performed on random-bred mice. Data from random-bred mice rather than those from inbred mice have been used because the error components can be expected to be larger and, consequently, more easily detectable. The model of errors has also been validated using data published by Till (1972) and has subsequently been applied to data from several inbred mice strains (Znojil & Necas, 1988).

• MeSH
• analýza kolonii tvořících jednotek normy   MeSH
• hematopoetické kmenové buňky cytologie   MeSH
• myši inbrední ICR MeSH
• myši MeSH
• statistika jako téma MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

A refinement of the method for determining the fraction of CFU-s in the S phase is presented. The new procedures were tested by means of a model experiment in which the fraction of CFU-s 'killed' was simulated by diluting the cell suspension. Another test was to determine the fraction of the CFU-s in the S phase using duplicate samples.

• MeSH
• analýza kolonii tvořících jednotek metody   MeSH
• DNA biosyntéza   MeSH
• hematopoetické kmenové buňky cytologie   MeSH
• inbrední kmeny myší MeSH
• interfáze MeSH
• myši MeSH
• slezina cytologie   MeSH
• thymidin toxicita   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• DNA MeSH
• thymidin MeSH

To determine the toxic effect of aflatoxin B1 (AFB1) on granulopoiesis in vitro, cultures of myeloid progenitor cells for granulocytes and macrophages CFU-GM in semisolid agar medium were used to evaluate colony formation and tritiated thymidine suicide technique for analysis of cycling status of CFU-GM. Male Fischer rats, 5 to 6 weeks old, were given a single i.p. injection of 1 mg/kg or 0.1 mg/kg of AFB1 which were approximately 1/5 or 1/50, respectively, of LD50 dose of AFB1. The administration of either dose of AFB1 caused a significant reduction of the number of CFU-GM derived colonies on the first and the second day after injection. This reduction was followed by a strong enhancement in CFU-GM colony formation on the third day, and by restoration to the normal level on the fourth day. The increase in percentage of CFU-GM in S-phase preceded one day the peak in CFU-GM colony formation. The two different doses of AFB1 exerted similar effects on the growth and cycling status of CFU-GM. Our data outline an early suppressive effect exerted by AFB1 to the rat granulopoiesis in vivo.

• MeSH
• aflatoxin B1 farmakologie   MeSH
• buněčné dělení účinky léků   MeSH
• buněčné klony MeSH
• buněčný cyklus účinky léků   MeSH
• buňky kostní dřeně MeSH
• dimethylsulfoxid farmakologie   MeSH
• granulocyty cytologie   účinky léků   MeSH
• hematopoetické kmenové buňky cytologie   účinky léků   MeSH
• hematopoéza účinky léků   MeSH
• injekce intraperitoneální MeSH
• kostní dřeň účinky léků   MeSH
• krysa rodu Rattus MeSH
• kultivované buňky MeSH
• monocyty cytologie   účinky léků   MeSH
• potkani inbrední F344 MeSH
• thymidin metabolismus   MeSH
• tritium MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• aflatoxin B1 MeSH
• dimethylsulfoxid MeSH
• thymidin MeSH
• tritium MeSH

A mathematical model of errors of the spleen colony technique is applied to data obtained from four mouse strains and F1 hybrids. The variance of the colony counts was close to the Poisson distribution in inbred mice and F1 hybrids. However, it should be checked regularly. The magnitude of the error in CFU-s determination and of the estimations of the S phase fraction was derived, and is presented relative to the mean colony counts for all mouse strains studied. The optimum spleen colony counts are generally higher than those which are commonly used. However, the utilization of the optimum spleen colony counts requires a correction for the effect of colony overlap.

• MeSH
• analýza kolonii tvořících jednotek metody   MeSH
• hematopoetické kmenové buňky cytologie   MeSH
• inbrední kmeny myší MeSH
• interfáze MeSH
• myši MeSH
• slezina cytologie   MeSH
• teoretické modely MeSH
• thymidin toxicita   MeSH
• viabilita buněk účinky léků   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• thymidin MeSH

The effect of sheep red blood cells (SRBC) and human red blood cells (HRBC) on the number of CFU-S in the bone marrow and spleen of (CBA X C57BL/6)F1 mice tolerant to SRBC was examined. The number of bone marrow and spleen CFU-S in SRBC tolerant mice was increased after injection of HRBC. After challenge with SRBC, CFU-S number was elevated in the spleen but not in the bone marrow. Thus, the mechanism of CFU-S activation in response to antigen exposure is different in the bone marrow and spleen.

• MeSH
• analýza kolonii tvořících jednotek MeSH
• antigeny imunologie   MeSH
• buňky kostní dřeně MeSH
• erytrocyty imunologie   MeSH
• hematopoetické kmenové buňky imunologie   MeSH
• imunologická tolerance * MeSH
• kostní dřeň imunologie   MeSH
• myši inbrední C57BL MeSH
• myši inbrední CBA MeSH
• myši MeSH
• ovce imunologie   MeSH
• slezina cytologie   imunologie   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antigeny MeSH

The recovery ability of colony-forming units from mice continuously irradiated for 30 and 100 days at an exposure rate of 25 and 50R/day was investigated. The rate of recovery depended not only on the daily exposure but also on the total dose accumulated. The course of the recovery process was assumed as follows: B(t) = Bs + (Bo--Bs). (formula:see text) was characterized by calculating the estimate(s) of the recovery coefficient tau.

• MeSH
• hematopoetické kmenové buňky účinky záření   MeSH
• hematopoéza účinky záření   MeSH
• kostní dřeň účinky záření   MeSH
• myši MeSH
• vztah dávky záření a odpovědi MeSH
• záření gama MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

A suspension of bone marrow cells from femurs of female (CBA X C57Bl)F1 mice was exposed to 2450 MHz CW microwaves in a specially designed waveguide exposure system. The temperature of the suspension rose, during exposure to microwaves, from 20 degrees C to 45 degrees C, and at an interval within 20 degrees C to 45 degrees C the number of haemopoietic stem cells (CFUs) was determined by the spleen exocolony method. The time of exposure of bone marrow cells to each temperature studied was 20 s. Control suspensions of bone marrow cells were exposed to a water bath temperature. There were no significant effects of the CFUs with the water bath temperature, while after exposure to microwaves the number of spleen colonies was elevated with a nadir at the temperature of 37 degrees C. With a microwave-induced increase of the temperature above 41 degrees C the number of CFUs in the bone marrow suspension decreased. The increase in the number of colonies was related to the rise in the seeding rate of the CFUs as well as to a rise in their proliferative activity, while the drop in the number of colonies was influenced also by heat-killing of the CFUs by microwave exposure.

• MeSH
• analýza kolonii tvořících jednotek MeSH
• buňky kostní dřeně MeSH
• dávka záření MeSH
• hematopoetické kmenové buňky fyziologie   účinky záření   MeSH
• idoxuridin MeSH
• mikrovlny * MeSH
• myši inbrední C57BL MeSH
• myši inbrední CBA MeSH
• myši MeSH
• slezina cytologie   MeSH
• teplota MeSH
• transplantace kostní dřeně MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• idoxuridin MeSH

The effect of rabbit anti-mouse brain serum and monospecific antibodies to an antigen of erythroblasts, Ag-Eb, on cells forming haemopoietic colonies on glass coverslips was studied. These colonies developed on the fibroblast-macrophage layer formed on glass coverslips which had been inserted into the peritoneal cavity of mice. when bone marrow cell suspensions were treated with RAMBS and the complement, or with anti-Ag-Eb antibodies plus complement, the CFU-GC colony-forming ability remained unaffected. In the examined cell suspensions, RAMBS inactivated 44% of CFU-S. When bone marrow cell suspensions were treated with anti-Ag-Eb antibodies, the number of immature erythroid cells was reduced to 75%. The CFU-GC are presumed to resemble the committed (unipotent) haemopoietic progenitor cells.

• MeSH
• analýza kolonii tvořících jednotek * MeSH
• antigeny analýza   MeSH
• antisérum imunologie   MeSH
• erytroblasty imunologie   MeSH
• hematopoetické kmenové buňky imunologie   fyziologie   MeSH
• inbrední kmeny myší MeSH
• komplement imunologie   MeSH
• kostní dřeň imunologie   MeSH
• králíci MeSH
• lidé MeSH
• myši MeSH
• peritoneální dutina * MeSH
• sklo MeSH
• zvířata MeSH
• Check Tag
• králíci MeSH
• lidé MeSH
• mužské pohlaví MeSH
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antigeny MeSH
• antisérum MeSH
• komplement MeSH