This paper presents results of experiments on the effects of electromagnetic radiation in the millimeter range (frequency 34.0 +/- 0.1 GHz, power density 20 muW/cm2) emitted by a police radar device. Considering the physical properties of the radiation in millimeter range (skin effects), the experiments were carried out on hairless mice. The main physiological parameters tested were body mass, body temperature, peripheral blood, and mass and cellularity of several important organs. Critical organs, the skin, and cornea were examined by electron microscopy. Differentiation ability of hematopoietic cells, progenitors of granulocytes and macrophages, and DNA synthesis in the cornea were compared in irradiated and nonirradiated animals. None of the parameters tested was affected to an extent that would indicate the start of a pathological process or the risk of damage to genetic material.
- MeSH
- elektromagnetické jevy * MeSH
- femur cytologie účinky záření MeSH
- krev účinky záření MeSH
- kůže účinky záření MeSH
- myši bezsrsté MeSH
- myši MeSH
- policie * MeSH
- radar * MeSH
- rohovka cytologie účinky záření MeSH
- tělesná hmotnost účinky záření MeSH
- tělesná teplota účinky záření MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Ventricular arrhythmias are an important cause of morbidity and mortality and come in a variety of forms, from single premature ventricular complexes to sustained ventricular tachycardia and fibrillation. Rapid developments have taken place over the past decade in our understanding of these arrhythmias and in our ability to diagnose and treat them. The field of catheter ablation has progressed with the development of new methods and tools, and with the publication of large clinical trials. Therefore, global cardiac electrophysiology professional societies undertook to outline recommendations and best practices for these procedures in a document that will update and replace the 2009 EHRA/HRS Expert Consensus on Catheter Ablation of Ventricular Arrhythmias. An expert writing group, after reviewing and discussing the literature, including a systematic review and meta-analysis published in conjunction with this document, and drawing on their own experience, drafted and voted on recommendations and summarized current knowledge and practice in the field. Each recommendation is presented in knowledge byte format and is accompanied by supportive text and references. Further sections provide a practical synopsis of the various techniques and of the specific ventricular arrhythmia sites and substrates encountered in the electrophysiology lab. The purpose of this document is to help electrophysiologists around the world to appropriately select patients for catheter ablation, to perform procedures in a safe and efficacious manner, and to provide follow-up and adjunctive care in order to obtain the best possible outcomes for patients with ventricular arrhythmias.
- Klíčová slova
- Catheter ablation, Clinical document, Electrical storm, Electroanatomical mapping, Electrocardiogram, Expert consensus statement, Imaging, Premature ventricular complex, Radiofrequency ablation, Ventricular arrhythmia, Ventricular tachycardia,
- MeSH
- katetrizační ablace * MeSH
- komorová tachykardie * chirurgie MeSH
- komorové extrasystoly * MeSH
- konsensus MeSH
- lidé MeSH
- srdeční elektrofyziologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- metaanalýza MeSH
- systematický přehled MeSH
- MeSH
- aklimatizace imunologie MeSH
- ascitická tekutina imunologie MeSH
- fagocyty imunologie MeSH
- imunokompetence MeSH
- kůže imunologie MeSH
- lymfatické uzliny imunologie MeSH
- lymfocyty imunologie MeSH
- myši bezsrsté imunologie MeSH
- myši inbrední BALB C MeSH
- myši nahé imunologie MeSH
- myši MeSH
- nízká teplota * MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Ventricular arrhythmias are an important cause of morbidity and mortality and come in a variety of forms, from single premature ventricular complexes to sustained ventricular tachycardia and fibrillation. Rapid developments have taken place over the past decade in our understanding of these arrhythmias and in our ability to diagnose and treat them. The field of catheter ablation has progressed with the development of new methods and tools, and with the publication of large clinical trials. Therefore, global cardiac electrophysiology professional societies undertook to outline recommendations and best practices for these procedures in a document that will update and replace the 2009 EHRA/HRS Expert Consensus on Catheter Ablation of Ventricular Arrhythmias. An expert writing group, after reviewing and discussing the literature, including a systematic review and meta-analysis published in conjunction with this document, and drawing on their own experience, drafted and voted on recommendations and summarized current knowledge and practice in the field. Each recommendation is presented in knowledge byte format and is accompanied by supportive text and references. Further sections provide a practical synopsis of the various techniques and of the specific ventricular arrhythmia sites and substrates encountered in the electrophysiology lab. The purpose of this document is to help electrophysiologists around the world to appropriately select patients for catheter ablation, to perform procedures in a safe and efficacious manner, and to provide follow-up and adjunctive care in order to obtain the best possible outcomes for patients with ventricular arrhythmias.
- Klíčová slova
- Catheter ablation, Clinical document, Electrical storm, Electroanatomical mapping, Electrocardiogram, Expert consensus statement, Imaging, Premature ventricular complex, Radiofrequency ablation, Ventricular arrhythmia, Ventricular tachycardia,
- MeSH
- elektrofyziologické techniky kardiologické metody MeSH
- katetrizační ablace * přístrojové vybavení metody normy MeSH
- komorová tachykardie * diagnóza etiologie patofyziologie chirurgie MeSH
- komorové extrasystoly * diagnóza etiologie patofyziologie chirurgie MeSH
- konsensus MeSH
- lidé MeSH
- mezinárodní spolupráce MeSH
- nemoci srdce klasifikace komplikace MeSH
- převodní systém srdeční patologie patofyziologie chirurgie MeSH
- společnosti lékařské MeSH
- srdeční elektrofyziologie * organizace a řízení normy trendy MeSH
- výsledek terapie MeSH
- zlepšení kvality organizace a řízení MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Mild cold acclimation (22 degrees C, 3 weeks) of hairless mice was shown to increase 5-fold the brown adipose tissue uncoupling protein content in immunodeficient BALB/c nu/nu mice, but by only 2.3-fold in immunocompetent BFU mice. The difference in activation of brown adipose tissue thermogenic capacity was due to a 2-fold increase in the content of brown adipose tissue in nu/nu mice only, which was paralleled by an increase in brown adipose tissue protein but not DNA content. Likewise, only in nu/nu mice the cold acclimation increased the reaction of natural killer cells in blood and peritoneal exudate with a shift from spleen to lymph nodes and increased the phagocytic index. The results indicate that the immune system may influence the defence against cold at the level of brown adipose tissue thermogenesis.
- MeSH
- aklimatizace MeSH
- buňky NK fyziologie MeSH
- fagocytóza MeSH
- hnědá tuková tkáň metabolismus fyziologie MeSH
- imunokompetence * MeSH
- myši bezsrsté fyziologie MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- nemoci imunitního systému patofyziologie MeSH
- nízká teplota * MeSH
- termoregulace fyziologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
UVA photons are less energetic than UVB photons but they are more abundant in solar radiation. Modern tools have shown that UVA light has serious adverse effects on the skin. We investigated the effect of consuming Lonicera caerulea berries on UVA-induced damage in SKH-1 mice. The mice were fed a diet containing L. caerulea berries (10%, w/w) for 14 days before a single UVA (30 J/cm(2)) treatment. Effects on haematological and antioxidant parameters were evaluated 4 and 24h after irradiation. The bioavailability of L. caerulea phenolics was also assessed. Consuming the L. caerulea berry-enriched diet caused reduced malondialdehyde production and increased catalase activity and glutathione levels were found in skin and erythrocytes. UVA-induced NADPH:quinone oxidoreductase-1 and gamma-L-glutamate-L-cysteine ligase protein in skin were reduced in mice fed L. caerulea berries. Enhanced heme oxygenase-1 level in skin, interleukin-17 in plasma and reduced interleukin-12 levels in plasma were found in the mice on the experimental diet. Histological (pyknotic) changes in the nuclei of basal cells induced by UVA exposure were reduced in L. caerulea berry consuming animals. HLPC-MS analysis showed high concentrations of hippuric acid, one of the main metabolites of aromatic amino acids and phenolic compounds, in skin, liver, urine and faeces of mice consuming the berries. Taken together, consumption of L. caerulea berries affords protection from the adverse effects of a single UVA exposure mainly via modulation of antioxidant parameters.
- Klíčová slova
- Blue honeysuckle, Erythrocyte, Hairless mice, Liver, Skin, UVA radiation,
- MeSH
- antioxidancia metabolismus MeSH
- dieta * MeSH
- enzymy metabolismus MeSH
- erytrocyty metabolismus účinky záření MeSH
- glutathion metabolismus MeSH
- hippuráty analýza moč MeSH
- interleukin-12 krev MeSH
- interleukin-17 krev MeSH
- játra chemie MeSH
- kůže metabolismus patologie účinky záření MeSH
- Lonicera chemie metabolismus MeSH
- malondialdehyd metabolismus MeSH
- myši bezsrsté MeSH
- myši MeSH
- ovoce chemie metabolismus MeSH
- ultrafialové záření * MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antioxidancia MeSH
- enzymy MeSH
- glutathion MeSH
- hippuráty MeSH
- hippuric acid MeSH Prohlížeč
- interleukin-12 MeSH
- interleukin-17 MeSH
- malondialdehyd MeSH
BACKGROUND: Solar light generates inflammatory responses in exposed skin. These effects are generally attributed to UVB light. However, skin is expose d to a huge quantum of UVA photons as UVA is a predominant part of sunlight and the radiation used in tanning beds. We examined the effects of a single exposure to UVA and UVB wavebands on cytokine levels in skin and plasma, myeloperoxidase (MPO) activity, expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) in skin. METHODS: Hairless mice were irradiated with either UVA (10 or 20 J/cm²) or UVB (200 or 800 mJ/cm²). The effects were assessed after 4/24 h. Plasma cytokine levels were evaluated using a Bio-Plex cytokine assay. Cytokine, iNOS and COX-2 levels in skin were determined by Western blot. Skin MPO activity was monitored spectrophotometrically. RESULTS: UVB induced up-regulation of interleukin-1β (IL-1β) and interleukin-6 (IL-6) and decrease in interleukin-10 (IL-10) mainly after 4 h. In contrast, UVA caused increase in levels of tumor necrosis factor-alpha (TNF-α) and IL-6 after 4 h and up-regulated IL-10 and interleukin-12 (IL-12) after 24 h. The increase in MPO activity from infiltrated leucocytes was observed only in UVB irradiated animals. iNOS was up-regulated 4 h after UVA and UVB treatment. No significant effect on COX-2 expression was detected. CONCLUSIONS: UVA and UVB light affected several inflammatory markers. For individual waveband, changes in plasma parameters did not correlate with those in skin. Thus evaluation of plasma samples cannot simply be replaced by determination in skin specimens and vice versa.
- MeSH
- časové faktory MeSH
- cyklooxygenasa 2 metabolismus účinky záření MeSH
- cytokiny * krev účinky záření MeSH
- kůže * metabolismus účinky záření MeSH
- myši bezsrsté MeSH
- myši MeSH
- peroxidasa metabolismus účinky záření MeSH
- spektrofotometrie MeSH
- synthasa oxidu dusnatého, typ II metabolismus účinky záření MeSH
- ultrafialové záření škodlivé účinky MeSH
- western blotting MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- cyklooxygenasa 2 MeSH
- cytokiny * MeSH
- peroxidasa MeSH
- synthasa oxidu dusnatého, typ II MeSH
- MeSH
- aklimatizace imunologie MeSH
- CD4-pozitivní T-lymfocyty imunologie MeSH
- CD8-pozitivní T-lymfocyty imunologie MeSH
- imunoglobulin G biosyntéza MeSH
- imunoglobulin M biosyntéza MeSH
- imunokompetence * MeSH
- myši bezsrsté imunologie MeSH
- myši inbrední BALB C MeSH
- myši nahé imunologie MeSH
- myši MeSH
- nízká teplota * MeSH
- tvorba protilátek * MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- imunoglobulin G MeSH
- imunoglobulin M MeSH
Schistosome cercariae are able to penetrate into the skin of various vertebrate hosts. However, in contrast to the compatible host, the infection of incompatible hosts results in the death of parasites at various intervals post-infection. In order to compare the immune responses in both types of host infected with Trichobilharzia regenti, Trichobilharzia szidatior Schistosoma mansoni, antibody responses against various T. regenti, T. szidatiand S. mansonischistosome developmental stages were studied. Indirect immunofluorescence tests (IFAT) demonstrated no species-specific reactivity of human, mouse or duck immune sera with cercarial surfaces. Study of the cercarial glands also gave no significant results. However, differences were found in schistosomular and adult antigens: only the sera of compatible hosts recognised schistosomular and adult gut associated antigens in homologous as well as heterologous systems. Based on the presented data, our study supports the use of IFAT for the serological differentiation of schistosomiasis and cercarial dermatitis caused by bird schistosomes.
- MeSH
- antigeny helmintové imunologie MeSH
- fluorescenční protilátková technika * MeSH
- interakce hostitele a parazita MeSH
- kachny MeSH
- myši bezsrsté MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- ovum imunologie ultrastruktura MeSH
- protilátky helmintové imunologie MeSH
- Schistosoma mansoni růst a vývoj imunologie metabolismus MeSH
- Schistosomatidae růst a vývoj imunologie metabolismus MeSH
- stadia vývoje MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antigeny helmintové MeSH
- protilátky helmintové MeSH
Ventricular arrhythmias are an important cause of morbidity and mortality and come in a variety of forms, from single premature ventricular complexes to sustained ventricular tachycardia and fibrillation. Rapid developments have taken place over the past decade in our understanding of these arrhythmias and in our ability to diagnose and treat them. The field of catheter ablation has progressed with the development of new methods and tools, and with the publication of large clinical trials. Therefore, global cardiac electrophysiology professional societies undertook to outline recommendations and best practices for these procedures in a document that will update and replace the 2009 EHRA/HRS Expert Consensus on Catheter Ablation of Ventricular Arrhythmias. An expert writing group, after reviewing and discussing the literature, including a systematic review and meta-analysis published in conjunction with this document, and drawing on their own experience, drafted and voted on recommendations and summarized current knowledge and practice in the field. Each recommendation is presented in knowledge byte format and is accompanied by supportive text and references. Further sections provide a practical synopsis of the various techniques and of the specific ventricular arrhythmia sites and substrates encountered in the electrophysiology lab. The purpose of this document is to help electrophysiologists around the world to appropriately select patients for catheter ablation, to perform procedures in a safe and efficacious manner, and to provide follow-up and adjunctive care in order to obtain the best possible outcomes for patients with ventricular arrhythmias.
- Klíčová slova
- Catheter ablation, Clinical document, Electrical storm, Electroanatomical mapping, Electrocardiogram, Expert consensus statement, Imaging, Premature ventricular complex, Radiofrequency ablation, Ventricular arrhythmia, Ventricular tachycardia,
- MeSH
- kardiologie * MeSH
- katetrizační ablace normy MeSH
- komorová tachykardie chirurgie MeSH
- konsensus * MeSH
- lidé MeSH
- společnosti lékařské * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
- Research Support, N.I.H., Extramural MeSH
