In this proof-of-concept study, we explore the detection of pesticides in food using a combined power of sensitive UV-induced fingerprint spectroscopy with selective capture by molecularly imprinted polymers (MIPs) and portable cost-effective paper-based analytical devices (PADs). The specific pesticides used herein as model compounds (both pure substances and their application products for spraying), were: strobilurins (i.e. trifloxystrobin), urea pesticides (rimsulfuron), pyrethroids (cypermethrine) and aryloxyphenoxyproponic acid herbicides (Haloxyfop-methyl). Commercially available spraying formulations containing the selected pesticides were positively identified by MIP-PADs swabs of sprayed apple and tomato. The key properties of MIP layer - imprinting factor (IF) and selectivity factor (α) were characterized using trifloxystrobin (IF-3.5, α-4.4) was demonstrated as a potential option for in-field application. The presented method may provide effective help with in-field testing of food and reveal problems such as false product labelling.

• Klíčová slova
• Fingerprint, Molecularly imprinted polymers, Pesticides, Photochemistry, UV-induced fluorescence fingerprinting,
• MeSH
• fluorescenční spektrometrie MeSH
• molekulárně imprintované polymery MeSH
• molekulový imprinting * MeSH
• pesticidy * analýza   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• molekulárně imprintované polymery MeSH
• pesticidy * MeSH

Nowadays about 28 million different simple or complex chemical entities are known. Today we cannot imagine the life without different kinds of drugs, cosmetics, pesticides, food additives or stimulants. In the recent period in the field of analytical chemistry or pharmacy more modern techniques or methodologies are required which will allow selective determination of different kinds of analytes, especially in complex biological matrices. The imprinted polymers are very often used for the preparation of samples before analysis and this procedure can reduce the possibilities of interferences. This paper deals with the characterization, preparation, properties and application of imprinted polymers in the field of drugs, cosmetics, food and biological materials.

• MeSH
• molekulový imprinting * MeSH
• polymery * chemie   MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• polymery * MeSH

We report a facile method for detection of metallothionein (MT), a promising clinically relevant biomarker, in spiked plasma samples. This method, for the first time, integrates molecularly imprinted polymers as purification/pretreatment step with matrix assisted laser desorption/ionization time-of-flight mass spectrometric detection and with laser ablation inductively coupled plasma mass spectrometry for analysis of MTs. The prepared MT-imprinted polydopamine layer showed high binding capacity and specific recognition properties toward the template. Optimal monomer (dopamine) concentration was found to be 16 mM of dopamine. This experimental setup allows to measure µM concentrations of MT that are present in blood as this can be used for clinical studies recognizing MT as marker of various diseases including tumour one. Presented approach not only provides fast sample throughput but also avoids the limitations of methods based on use of antibodies (e.g. high price, cross-reactivity, limited availability in some cases, etc.).

• Klíčová slova
• LA-ICP-MS, MALDI-TOF-MS, Metallothionein, Molecularly imprinted polymers, Polydopamine,
• MeSH
• hmotnostní spektrometrie MeSH
• indoly chemie   MeSH
• lidé MeSH
• metalothionein krev   MeSH
• molekulový imprinting * MeSH
• polymery chemie   MeSH
• zdraví dobrovolníci pro lékařské studie MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• indoly MeSH
• metalothionein MeSH
• polydopamine MeSH Prohlížeč
• polymery MeSH

Molecularly imprinted polymers (MIPs) are currently widely used and further developed for biological applications. The MIP synthesis procedure is a key process, and a wide variety of protocols exist. The templates that are used for imprinting vary from the smallest glycosylated glycan structures or even amino acids to whole proteins or bacteria. The low cost, quick preparation, stability and reproducibility have been highlighted as advantages of MIPs. The biological applications utilizing MIPs discussed here include enzyme-linked assays, sensors, in vivo applications, drug delivery, cancer diagnostics and more. Indeed, there are numerous examples of how MIPs can be used as recognition elements similar to natural antibodies.

• MeSH
• biologické markery analýza   MeSH
• lékové transportní systémy MeSH
• molekulárně imprintované polymery chemická syntéza   chemie   MeSH
• nádory patologie   MeSH
• polymerizace MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• biologické markery MeSH
• molekulárně imprintované polymery MeSH

In this work, a novel method was developed, for isolation of S. aureus from complex (food) samples using molecular imprinting. Dopamine was used as a functional monomer and fluorescence microscopy was used for detection. Conditions for preparation of molecularly imprinted polymers (MIPs), adsorption performance, adsorption kinetic, and selectivity of the polymeric layers were investigated. The various procedures were combined in a single extraction process, with the imprinted layer on the surface of the magnetic particles (magnetic MIPs). Subsequently, MIPs were used for extraction of S. aureus from milk and rice. Moreover, raw milk from cows with mastitis was tested successfully. Using this novel MIP-based method, it was possible to detect bacteria in milk at 1 × 103CFU·ml-1, which corresponds to the limit set in European Union legislation for microbial control of food.

• Klíčová slova
• Bacteria, Dopamine, Fluorescence microscopy, Magnetic particles, Molecularly imprinted polymers,
• MeSH
• adsorpce MeSH
• extrakce na pevné fázi MeSH
• magnetické jevy MeSH
• magnetismus MeSH
• mléko mikrobiologie   MeSH
• molekulový imprinting metody   MeSH
• polymery chemie   MeSH
• skot MeSH
• Staphylococcus aureus izolace a purifikace   MeSH
• zvířata MeSH
• Check Tag
• skot MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• polymery MeSH

Medical diagnostics aims at specific localization of molecular targets as well as detection of abnormalities associated with numerous diseases. Molecularly imprinted polymers (MIPs) represent an approach of creating a synthetic material exhibiting selective recognition properties toward the desired template. The fabricated target-specific MIPs are usually well reproducible, economically efficient, and stable under critical conditions as compared to routinely used biorecognition elements such as fluorescent proteins, antibodies, enzymes, or aptamers and can even be created to those targets for which no antibodies are available. In this review, we summarize the methods of polymer fabrication. Further, we provide key for selection of the core material with imaging function depending on the imaging modality used. Finally, MIP-based imaging applications are highlighted and presented in a comprehensive form from different aspects. STATEMENT OF SIGNIFICANCE: In this review, we summarize the methods of polymer fabrication. Key applications of Molecularly imprinted polymers (MIPs) in imaging are highlighted and discussed with regard to the selection of the core material for imaging as well as commonly used imaging targets. MIPs represent an approach of creating a synthetic material exhibiting selective recognition properties toward the desired template. The fabricated target-specific MIPs are usually well reproducible, economically efficient, and stable under critical conditions as compared to routinely used biorecognition elements, e.g., antibodies, fluorescent proteins, enzymes, or aptamers, and can even be created to those targets for which no antibodies are available.

• Klíčová slova
• Affinity, Luminescence, Microscopy, Polymerization,
• MeSH
• fototermální terapie MeSH
• molekulárně imprintované polymery chemie   MeSH
• receptory umělé chemie   MeSH
• zobrazování trojrozměrné * MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• Názvy látek
• molekulárně imprintované polymery MeSH
• receptory umělé MeSH

A new analytical method based on capillary zone electrophoresis-tandem mass spectrometry is proposed and validated for the identification and simultaneous quantification of nine aminoglycosides in honey samples. Detection using an ion trap mass analyzer operating in the multiple reaction monitoring mode was used. Different parameters were optimized in order to obtain an adequate separation combined with the highest sensitivity. In order to achieve high selectivity in the sample treatment, a commercially-available molecularly imprinted polymer has been used for the solid phase extraction of the analytes. Under optimum conditions, recoveries for fortified samples ranged from 88.2 to 99.8%, with relative standard deviations lower than 8%. The limits of detection ranged from 0.4 to 28.5 μg kg(-1). Furthermore, the decision limit and the detection capability were evaluated, ranging from 3.5 to 60.5 μg kg(-1) and from 6.0 to 103.1 μg kg(-1), respectively, demonstrating the sensitivity and applicability of this fast and simple method.

• Klíčová slova
• Aminoglycosides, Capillary zone electrophoresis, Honey, Molecularly imprinted polymers, Stacking, Tandem mass spectrometry,
• MeSH
• aminoglykosidy analýza   MeSH
• elektroforéza kapilární metody   MeSH
• extrakce na pevné fázi metody   MeSH
• limita detekce MeSH
• med analýza   MeSH
• molekulový imprinting * metody   MeSH
• polymery chemie   MeSH
• tandemová hmotnostní spektrometrie metody   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• validační studie MeSH
• Názvy látek
• aminoglykosidy MeSH
• polymery MeSH

The release of pharmaceuticals into the environment induces adverse effects on the metabolism of humans and other living species, calling for advanced remediation methods. Conventional removal methods are often non-selective and cause secondary contamination. These issues may be partly solved by the use of recently-developped adsorbents such as molecularly imprinted polymers. Here we review the synthesis and application of molecularly imprinted polymers for removing pharmaceuticals in water. Molecularly imprinted polymers are synthesized via several multiple-step polymerization methods. Molecularly imprinted polymers are potent adsorbents at the laboratory scale, yet their efficiency is limited by template leakage and polymer quality. Adsorption performance of multi-templated molecularly imprinted polymers depends on the design of wastewater treatment plants, pharmaceutical consumption patterns and the population serviced by these wastewater treatment plants.

• Klíčová slova
• Contaminated water, Limitations, Molecularly imprinted materials, Pharmaceuticals, Remediation,
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH

Advanced solid phase extraction (SPE) fibrous sorbents including polyethylene, polypropylene poly (hydroxybutyrate), and polyamide 6 nanofibers, polycaprolactone microfibers/nanofibers, polycaprolactone microfibers/polyvinylidene difluoride nanofibers, and poly (hydroxybutyrate) microfibers/polypropylene microfibers composites, as well as commercial molecularly imprinted polymers and restricted access media sorbent were compared in terms of bisphenols extraction from milk and their clean-up efficiency. Three on-line SPE-HPLC methods were completely validated for the extraction and detection of bisphenols A, AF, C, A diglycidyl ether, and F diglycidyl ether in bovine milk. Polycaprolactone composite nanofibers compared favorably to restricted access media, enabled excellent clean-up of bisphenols from the proteinaceous matrix, and yielded recoveries 98.0-124.5% and 93.0-115.0%, respectively, with RSD less than 10%. Total analysis time including on-line SPE step lasted only 12 min, which represents a significant reduction in time compared with previously reported as well as official European Union and AOAC methods defined for the determination of bisphenols in various matrices.

• Klíčová slova
• Bisphenol, Milk, Molecularly imprinted polymer, Nanofiber, On-line SPE-HPLC, Restricted access media,
• MeSH
• adsorpce MeSH
• ethery MeSH
• extrakce na pevné fázi metody   MeSH
• hydroxybutyráty MeSH
• mléko MeSH
• molekulárně imprintované polymery MeSH
• molekulový imprinting * metody   MeSH
• nanovlákna * chemie   MeSH
• polypropyleny MeSH
• vysokoúčinná kapalinová chromatografie metody   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• bisphenol A MeSH Prohlížeč
• ethery MeSH
• hydroxybutyráty MeSH
• molekulárně imprintované polymery MeSH
• polypropyleny MeSH

The paper reviews recent developments in the use of molecularly imprinted polymers in several analytical techniques, such as solid-phase extraction, liquid chromatography, capillary electrophoresis, capillary electrochromatography, and as selective sorbents in chemical sensors. Molecular imprinting is achieved by the interaction between complementary groups in a template molecule and functional monomer units. The benefits of imprinted polymers are low cost, storage stability, high mechanical strength, repeated operations without loss of activity, and potential application to a wide range of target molecules.

• MeSH
• chemické techniky analytické MeSH
• farmaceutická chemie * metody   MeSH
• molekulový imprinting * metody   MeSH
• polymery * MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• Názvy látek
• polymery * MeSH